Monkey Pox Virus (MPXV): Phylogenomics, Host-Pathogen Interactome, and Mutational Cascade

Abstract

While the world is still managing to recover from Covid-19 pandemic, Monkeypox awaits to bring in another global outbreak as a challenge to the entire mankind. However, Covid-19 pandemic have taught us lessons to move fast in viral genomic research to implement prevention and treatment strategies. One of the important aspects in Monkeypox virus should be immediately taken up is to gather insights of its evolutionary lineage based on the genomic studies. We have thus analysed the genome sequences of reported isolates of Monkeypox in the present study through phylogenomics. Host-pathogen interactions, mutation prevalence and evolutionary dynamics of this virus were investigated for all the documented isolates. Phylogenetic exploration revealed the clustering of strain Israel 2018 (MN 648051.1) from Clade I with the four isolates reported from the recent outbreak. An in-depth scrutiny of the host-pathogen interactome identified protein E3, serine protease inhibitor-2 (SPI-2), protein K7, and cytokine response-modifying protein B (CrmB) as the major regulatory hubs. Among these, the CrmB protein (dN/dS ≈ 1.61) was detected to be operating through positive selection. It possibly attests a selective advantage with the monkeypox virus in protecting the infected cells from antiviral responses elicited by the host. Studies also revealed that CrmB protein exhibited several mutations, the majority of which were destabilizing (ΔΔG >0). While this study identified a large number of mutations within the newly outbreak clade, it also reflected that we need to move fast with the genomic analysis of the newly detected strains from around the world to develop better prevention and treatment methods.

Editorial: Multi-omics profiling of unique niches to reveal the microbial and metabolite composition

“…These include (meta)genomics, transcriptomics, proteomics, meta-metabolomics, and culturomics in different aspects of basic and applied research. The use of OMICS has greatly enhanced our understanding of the structure, structure-related functions, and interactions among microbes with biotic and abiotic components of ecosystems and revealed their role in offering different ecosystem services (Kumar et al, 2022). Considering the extensive use and versatility of OMICS techniques in different disciplines of life science, we created this Research Topic "Multi-omics profiling of unique niches to reveal the microbial and metabolite composition" to understand the role of microbes in their respective niches in the presence and absence of certain variables.…”

Formulation & Evaluation of Anti Migrane Mouth Dissolving Tablet

“…Migraine sufferers would benefit from acute treatment that enables them to resume functional activities as early as possible because of the severe impairment in their functional skills that they experience as a result of migraines. The availability of certain effective alternatives to solid dosage forms has been made possible as a result of technological advancements [3,4]. Patients who have difficulty swallowing or digesting solid dose forms, who are immobile, who feel sick, or who do not comply with their treatment can now take advantage of these alternatives.…”

Phylogeny and Molecular evolution of the first local Monkeypox virus cluster in Guangdong Province, China

“…Nevertheless, additional studies are required to verify whether the premature termination of A49R affects viral replication and transcription. Compared to the B.1 lineage in the MPX outbreak of early 2022, these mutations have promoted the rapid evolution and diversification of the current MPXV lineage [15,16].…”