Monkey Embryonic Stem Cell Lines Expressing Green Fluorescent Protein

Takada, Tatsuyuki; Suzuki, Yutaka; Kondo, Yasushi; Kadota, Nae; Kobayashi, Kinji; Nito, Shinji; Kimura, Hiroshi; Torii, Ryuzo

doi:10.3727/000000002783985350

Cited by 31 publications

(20 citation statements)

References 15 publications

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“…Synchronously pulsing EBs contain myogenic cells positive for ␣-cardiac actin, desmin, myogenin, and smooth muscle actin (44,234). Consistent with these in vitro observations, in vivo delivery of human ESC lines to skeletal muscle or the testis gives rise to teratomas (397,459,477). Teratomas include endodermal, mesodermal, and ectodermal structures formed by mature cells (Fig.…”

Section: A Human Embryonic Stem Cellssupporting

confidence: 57%

Cardiac Stem Cells and Mechanisms of Myocardial Regeneration

Leri

Kajstura²,

Anversa³

2005

Physiological Reviews

397

349

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This review discusses current understanding of the role that endogenous and exogenous progenitor cells may have in the treatment of the diseased heart. In the last several years, a major effort has been made in an attempt to identify immature cells capable of differentiating into cell lineages different from the organ of origin to be employed for the regeneration of the damaged heart. Embryonic stem cells (ESCs) and bone marrow-derived cells (BMCs) have been extensively studied and characterized, and dramatic advances have been made in the clinical application of BMCs in heart failure of ischemic and nonischemic origin. However, a controversy exists concerning the ability of BMCs to acquire cardiac cell lineages and reconstitute the myocardium lost after infarction. The recognition that the adult heart possesses a stem cell compartment that can regenerate myocytes and coronary vessels has raised the unique possibility to rebuild dead myocardium after infarction, to repopulate the hypertrophic decompensated heart with new better functioning myocytes and vascular structures, and, perhaps, to reverse ventricular dilation and wall thinning. Cardiac stem cells may become the most important cell for cardiac repair.

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Section: A Human Embryonic Stem Cellssupporting

confidence: 57%

Cardiac Stem Cells and Mechanisms of Myocardial Regeneration

Leri

Kajstura²,

Anversa³

2005

Physiological Reviews

397

349

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“…Stable ESC lines have been established in many mammalian species, including mouse (Evans and Kaufman, 1981), rat (Buehr et al, 2008;Li et al, 2008), monkey (Takada et al, 2002;Thomson et al, 1995), dog (Vaags et al, 2009), cattle , and human (Thomson et al, 1998). Despite all of these achievements and efforts, only murine and rat ESCs have successfully transmitted to the germ line (Buehr et al, 2008;Evans and Kaufman, 1981;Li et al, 2008).…”

mentioning

confidence: 99%

Recombinant Rabbit Leukemia Inhibitory Factor and Rabbit Embryonic Fibroblasts Support the Derivation and Maintenance of Rabbit Embryonic Stem Cells

Chen

Zhang

et al. 2012

Cellular Reprogramming

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The rabbit is a classical experimental animal species. A major limitation in using rabbits for biomedical research is the lack of germ-line-competent rabbit embryonic stem cells (rbESCs). We hypothesized that the use of homologous feeder cells and recombinant rabbit leukemia inhibitory factor (rbLIF) might improve the chance in deriving germ-line-competent rbES cells. In the present study, we established rabbit embryonic fibroblast (REF) feeder layers and synthesized recombinant rbLIF. We derived a total of seven putative rbESC lines, of which two lines (M5 and M23) were from culture Condition I using mouse embryonic fibroblasts (MEFs) as feeders supplemented with human LIF (hLIF) (MEF + hLIF). Another five lines (R4, R9, R15, R21, and R31) were derived from Condition II using REFs as feeder cells supplemented with rbLIF (REF + rbLIF). Similar derivation efficiency was observed between these two conditions (8.7% vs. 10.2%). In a separate experiment with 2 · 3 factorial design, we examined the effects of feeder cells (MEF vs. REF) and LIFs (mLIF, hLIF vs. rbLIF) on rbESC culture. Both Conditions I and II supported satisfactory rbESC culture, with similar or better population doubling time and colony-forming efficiency than other combinations of feeder cells with LIFs. Rabbit ESCs derived and maintained on both conditions displayed typical ESC characteristics, including ESC pluripotency marker expression (AP, Oct4, Sox2, Nanog, and SSEA4) and gene expression (Oct4, Sox2, Nanog, c-Myc, Klf4, and Dppa5), and the capacity to differentiate into three primary germ layers in vitro. The present work is the first attempt to establish rbESC lines using homologous feeder cells and recombinant rbLIF, by which the rbESCs were derived and maintained normally. These cell lines are unique resources and may facilitate the derivation of germline-competent rbESCs.

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“…We used green fluorescent protein (GFP)-expressing ES cells (GFP-ES) established and cultured as previously described (Takada et al 2002). These GFP-ES cells (about 12-to 13-lm diameter) differentiated into neural cells and cardiac myocytes in vitro and formed teratomas composed of tissue specimens derived from the three embryonic germ layers when transplanted into severe combined immunodeficient disease (SCID) mice (Takada et al 2002).…”

Section: Establishment and Culture Of Donor Cellsmentioning

confidence: 99%

“…These GFP-ES cells (about 12-to 13-lm diameter) differentiated into neural cells and cardiac myocytes in vitro and formed teratomas composed of tissue specimens derived from the three embryonic germ layers when transplanted into severe combined immunodeficient disease (SCID) mice (Takada et al 2002). For use as donor cells in NT, GFP-ES cells were selected by small size.…”

Section: Establishment and Culture Of Donor Cellsmentioning

confidence: 99%

Cloned blastocysts produced by nuclear transfer from somatic cells in cynomolgus monkeys (Macaca fascicularis)

et al. 2007

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In nonhuman primates (NHPs), there have so far been few reports about nuclear transfer (NT), especially using adult somatic cells. The objective of this study was to determine the developmental competence of NT embryos derived from various somatic cells embryonic stem (ES), amniotic epithelial, cumulus, or fetal fibroblast cells] and the nuclear transfer method, such as electro fusion or piezo microinjection, activation with chemical reagent [ionomycine/6-dimethylaminopurine (DMAP), calcium ionophore A23187/DMAP, or cycloheximide (CHX)] and reprogramming time (1, 2, or 4 h; in this study, the duration from injection or fusion to activation was defined as the reprogramming time). Our results showed that a 1-h reprogramming and activation with ionomycin/DMAP are suitable for NT in monkeys. Developing cleaved embryos up to the six-cell stage was similar among all experiments. However, beyond the eight-cell stage, developmental rates were higher in NT embryos reconstructed with fetal fibroblast cells and amniotic epithelial cells, and we were able to produce NT blastocysts from these cells. Interestingly, electro fusion is sufficient for amniotic epithelial cells and piezo microinjection is better suited for fetal fibroblast cells to produce NT blastocysts, thus suggesting that the best method for somatic cell NT may be different between cell types.

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Monkey Embryonic Stem Cell Lines Expressing Green Fluorescent Protein

Cited by 31 publications

References 15 publications

Cardiac Stem Cells and Mechanisms of Myocardial Regeneration

Cardiac Stem Cells and Mechanisms of Myocardial Regeneration

Recombinant Rabbit Leukemia Inhibitory Factor and Rabbit Embryonic Fibroblasts Support the Derivation and Maintenance of Rabbit Embryonic Stem Cells

Cloned blastocysts produced by nuclear transfer from somatic cells in cynomolgus monkeys (Macaca fascicularis)

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