Monitoring the VEGF level in aqueous humor of patients with ophthalmologically relevant diseases via ultrahigh sensitive paper-based ELISA

Hsu, Min‐Yen; Yang, Chung‐Yao; Hsu, Wei‐Li; Lin, Keng‐Hung; Wang, Chun-Yuan; Shen, Ying-Cheng; Chen, Yu‐Chen; Chau, Siu-Fung; Tsai, Hin-Yeung; Cheng, Chao‐Min

doi:10.1016/j.biomaterials.2014.01.030

Cited by 80 publications

(66 citation statements)

References 28 publications

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“…[20][21][22][23][24] Paper is low-cost, ubiquitous, and easily disposed of by incineration. It is also lightweight, highly compatible with current technologies, possesses natural capillary action, and has a high surface area to volume ratio, which contributes to the development of affordable and user-friendly PADs for rapid biochemical analysis.…”

Section: Introductionmentioning

confidence: 99%

Paper-based diagnostic devices for clinical paraquat poisoning diagnosis

et al. 2016

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This article unveils the development of a paper-based analytical device designed to rapidly detect and clinically diagnose paraquat (PQ) poisoning. Using wax printing technology, we fabricated a PQ detection device by pattering hydrophobic boundaries on paper. This PQ detection device employs a colorimetric sodium dithionite assay or an ascorbic acid assay to indicate the PQ level in a buffer system or in a human serum system in 10 min. In this test, colorimetric changes, blue in color, were observable with the naked eye. By curve fitting models of sodium dithionite and ascorbic acid assays in normal human serum, we evaluated serum PQ levels for five PQ-poisoned patients before hemoperfusion (HP) treatment and one PQpoisoned patient after HP treatment. As evidenced by similar detection outcomes, the analytical performance of our device can compete with that of the highest clinical standard, i.e., spectrophotometry, with less complicated sample preparation and with more rapid results. Accordingly, we believe that our rapid PQ detection can benefit physicians determining timely treatment strategies for PQ-poisoned patients once they are taken to hospitals, and that this approach will increase survival rates.Published by AIP Publishing. [http://dx

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Section: Introductionmentioning

confidence: 99%

Paper-based diagnostic devices for clinical paraquat poisoning diagnosis

et al. 2016

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“…Moreover, lPADs can be fabricated with either hydrophobic or hydrophilic channels using a simple wax printer. [5][6][7][8][9][10][11] However, in performing biological assays using lPADs, it is essential that both the reaction duration and the intensity of the resulting colorimetric detection signal are carefully controlled so as to improve the sensitivity and specificity of the results. 12,13 Consequently, the problem of realizing timedelay valves on lPADs has attracted increasing attention in recent years.…”

Section: Introductionmentioning

confidence: 99%

Colored wax-printed timers for two-dimensional and three-dimensional assays on paper-based devices

et al. 2014

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Microfluidic paper-based analytical devices (lPADs) are widely used for performing diagnostic assays. However, in many assays, time-delay valves are required to improve the sensitivity and specificity of the results. Accordingly, this study presents a simple, low-cost method for realizing time-delay valves using a color wax printing process. In the proposed approach, the time-delay effect is controlled through a careful selection of both the color and the saturation of the wax content. The validity of the proposed method is demonstrated by performing nitrite and oxalate assays using both a simple two-dimensional lPAD and a three-dimensional lPAD incorporating a colored wax-printed timer. The experimental results confirm that the flow time can be controlled through an appropriate selection of the color and the wax content. In addition, it is shown that nitrite and oxalate assays can be performed simultaneously on a single device. In general, the results presented in this study show that the proposed lPADs provide a feasible low-cost alternative to conventional methods for performing diagnostic assays. V C 2014 AIP Publishing LLC.

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“…or an enzyme-linked immunosorbent assay (ELISA) [i.e., using antibodies to indicate specific target antigens, such as vascular endothelial growth factor (VEGF), hCG, NC16A, etc.] [2,5,[11][12][13]. Both PAD approaches offer promising strategies to ameliorate global health issues.…”

Section: Introductionmentioning

confidence: 99%