Monitoring the Succinate Dehydrogenase Activity Isolated from Mitochondria by Surface Enhanced Raman Scattering

Hollywood, Katherine A.; Shadi, I. T.; Goodacre, Royston

doi:10.1021/jp908950x

Cited by 30 publications

(21 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Both infrared and Raman spectroscopies have been extensively applied in various research areas (Thygesen et al 2003), such as for detection of food toxicants and chemical adulteration (Cheung et al 2010;Lin et al 2008;), bioprocessing and fermentation monitoring (Goodacre and Jarvis 2005;Brewster et al 2009;Clarke et al 2005;Shaw et al 1999), enzyme activity (Hollywood et al 2010), microorganism identification and segregation (Burgula et al 2007;Mariey et al 2001;Preisner et al 2007;Maquelin et al 2002;Naumann 2001;Harz et al 2009;Lu and Rasco 2010a;Huang et al 2010;Davis et al 2010), microbial cell injury identification (Lin et al 2004;Al-Qadiri et al 2008a), virus identification (Fan et al 2010), and prion structure elucidation (Beekes et al 2007). Fourier transforminfrared (FT-IR) spectroscopy has also been used to monitor microbial spoilage in food systems, such as chicken breast (Ellis et al 2002), beef (Ellis et al 2004), and milk (Nicolaou and Goodacre 2008).…”

mentioning

confidence: 99%

Application of Mid-infrared and Raman Spectroscopy to the Study of Bacteria

Al-Qadiri

Lin

et al. 2011

Food Bioprocess Technol

204

120

View full text Add to dashboard Cite

Infrared spectroscopy and Raman spectroscopy provide complementary technologies for rapid and precise detection of microorganisms and are emerging methods in food analysis. It is possible to use either of these techniques to differentiate and quantify microorganisms in relatively simple matrices such as liquid media and simple solutions with determinations taking less than an hour. Vibrational spectroscopy, unlike other techniques used in microbiology, is a relatively simple method for studying structural changes occurring within a microbial cell following environmental stress and applications of food processing treatments. Vibrational spectroscopy provides a wide range of biochemical properties about bacteria in a single spectrum, most importantly characteristics of the cell membrane. These techniques are especially useful for studying properties of bacterial biofilms on contact surfaces, the presence and viability of bacterial vegetative cells and spores, the type and degree of bacterial injury, and assessment of antibiotic susceptibility. Future trends in food analysis will involve combining vibrational spectroscopy with microscopy, mass spectroscopy, or DNA-based methods to comprehensively study bacterial stress. Further advances in selectivity, sensitivity, and improved chemometric methods, along with reduction in the cost of instrumentation, may lead to the development of fieldready and real-time analytical systems.

show abstract

mentioning

confidence: 99%

Application of Mid-infrared and Raman Spectroscopy to the Study of Bacteria

Al-Qadiri

Lin

et al. 2011

Food Bioprocess Technol

204

120

View full text Add to dashboard Cite

show abstract

“…Infrared and Raman spectroscopies have been extensively applied in various research areas: for detection of food toxicants and chemical adulteration [11][12][13], bioprocessing and fermentation monitoring [14][15][16][17], enzyme activity [18], microorganism identification and segregation [19][20][21][22][23][24][25][26], microbial cell injury identification [27,28], virus identification [29], and prion structure elucidation [30].…”

Section: Discussionmentioning

confidence: 99%

Differentiation of bacterial strains by means of surface enhanced FT-Raman spectroscopy

Kairyte¹,

Lukšienė²,

Pučetaitė³

et al. 2012

Lith. J. Phys.

View full text Add to dashboard Cite

The silver nanoparticle colloid was used to obtain surface enhanced Raman spectra of Listeria monocytogenes, Salmonela enterica, and Esherichia coli bacteria. The SERS spectra were captured using for excitation the near-infrared (1064 nm) laser radiation with reduced intensity, which ensured the prevention of the fluorescence background as well as photo-and thermal decomposition of the samples. It was found that the optimal size of silver nanoparticles for the enhancement of the Raman signal in the near-infrared spectral region is ca. 50 nm. The spectral data obtained in this study indicate that relative intensities of SERS spectral bands of bacteria can be used for spectral differentiation of bacteria. In case of Listeria, Salmonela, and Esherichia cells, the intensity ratio of spectral bands of adenine and cysteine can be used as a spectral marker for differentiation of the bacteria.

show abstract

“…The purity and integrity of the mitochondrial preparations were assesd by performing the lactate dehydrogenase (LDH), and succinate dehydrogenase (SDH) assays as reported earlier .…”

Section: Methodsmentioning

confidence: 99%

“…The SDH enzyme activity was measured to determine the structural integrity and functional status of the mitochondrial fractions as described by Hollywood et al with minor modifications. The reaction mixture for SDH assay consisted of 100 mM HEPES, 0.5 mM 2,6‐dichlorophenolindophenol (DCPIP), 0.4 M succinic acid and 25 μg of the mitochondrial pellet.…”

Section: Methodsmentioning

confidence: 99%

Detection of mitochondrial dysfunction in vitro by laser‐induced autofluorescence

Raghushaker

Chandra

Chakrabarty

et al. 2019

Journal of Biophotonics

View full text Add to dashboard Cite

Mitochondrion plays a significant role in a variety of biological functions. Because of their diverse character and location in the cellular systems, mitochondria commonly get exposed to various extrinsic and intrinsic cellular stresses. The present study reports a novel approach to detection of mitochondrial dysfunction based on tryptophan autofluorescence of its proteins in mouse liver, using laser-induced fluorescence (LIF) as a tool. Mitochondria, isolated from the mouse liver, were initially tested for purity and integrity using lactate dehydrogenase and succinate dehydrogenase (SDH) assays.Mitochondrial stress was induced by treating the isolated mitochondria with heavy metals at 10 and 0.01 mM for sodium arsenite and mercuric chloride, respectively. Upon treatment with the heavy metal, tryptophan autofluorescence quenching was recorded at 281 nm excitation. The functional integrity of the mitochondria treated with heavy metals was evaluated by measuring SDH and cytochrome c oxidase activities at various concentrations of mitochondria, which showed impaired activity as compared to control upto a concentration of 6.25 μg. A significant shift was also observed in the autofluorescence of proteins upto the level below 1 μg, suggesting their conformational change and hence altered structural integrity of mitochondria. Circular dichroism spectroscopy data of the mitochondrial proteins treated with heavy metals further validates their conformational change as compared to untreated control. The present study clearly shows that the LIF can be a novel detection tool to detect altered structural integrity of cellular mitochondria upon stress, and it also possesses the potentiality to combine with other interdisciplinary modalities. K E Y W O R D SANS fluorescence, heavy metals, LDH and SDH assay, tryptophan autofluorescence

show abstract

Monitoring the Succinate Dehydrogenase Activity Isolated from Mitochondria by Surface Enhanced Raman Scattering

Cited by 30 publications

References 25 publications

Application of Mid-infrared and Raman Spectroscopy to the Study of Bacteria

Application of Mid-infrared and Raman Spectroscopy to the Study of Bacteria

Differentiation of bacterial strains by means of surface enhanced FT-Raman spectroscopy

Detection of mitochondrial dysfunction in vitro by laser‐induced autofluorescence

Contact Info

Product

Resources

About