Monitoring the reaction of hemoglobin with hydrogen peroxide by capillary electrophoresis-chemiluminescence detection

Zhou, Shilai; Wang, Junhua; Huang, Wei‐Hua; Lu, Xin; Cheng, Jie

doi:10.1016/j.jchromb.2006.12.005

Cited by 13 publications

(8 citation statements)

References 32 publications

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“…The peroxidase mimetic activity of Hb in luminol/H 2 O 2 CL system is well documented presenting the applicability of the sensing concept for ample analytical detection approaches [17,[34][35][36][37][38]. Therefore, the incorporation of a catalyst onto magnetic support via surface chemistry should eliminate any opto-chemical response that can be translated to intensified background luminescence.…”

Section: Characterization Of Modified Mnpsmentioning

confidence: 99%

Ultrasensitive haptoglobin biomarker detection based on amplified chemiluminescence of magnetite nanoparticles

et al. 2020

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Background: Haptoglobin is an acute-phase protein used as predicting diagnostic biomarker both in humans (i.e., diabetes, ovarian cancer, some neurological and cardiovascular disorders) and in animals (e.g., bovine mastitis). The latter is a frequent disease of dairy industry with staggering economical losses upon decreased milk production and increased health care costs. Early stage diagnosis of the associated diseases or inflammation onset is almost impossible by conventional analytical manners. Results:The present study demonstrates a simple, rapid, and cost-effective label-free chemiluminescence bioassay based on magnetite nanoparticles (MNPs) for sensitive detection of haptoglobin by employing the specific interaction of hemoglobin-modified MNPs. The resulting haptoglobin-hemoglobin complex inhibits the peroxidase-like activity of luminol/H 2 O 2 -hemoglobin-MNPs sensing scheme and reduces the chemiluminescence intensities correspondingly to the innate haptoglobin concentrations. Quantitative detection of bovine haptoglobin was obtained within the range of 1 pg mL −1 to 1 µg mL −1 , while presenting 0.89 pg mL −1 limit of detection. Moreover, the influence of causative pathogenic bacteria (i.e., Streptococcus dysgalactiae and Escherichia coli) and somatic cell counts (depicting healthy, sub-clinical and clinical mastitis) on the emitted chemiluminescence radiation were established. The presented bioassay quantitative performances correspond with a standardized assay kit in differentiating dissimilar milk qualities. Conclusions:Overall, the main advantage of the presented sensing concept is the ability to detect haptoglobin, at clinically relevant concentrations within real milk samples for early bio-diagnostic detection of mastitis and hence adjusting the precise treatment, potentially initiating a positive influence on animals' individual health and hence on dairy farms economy.

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Section: Characterization Of Modified Mnpsmentioning

confidence: 99%

Ultrasensitive haptoglobin biomarker detection based on amplified chemiluminescence of magnetite nanoparticles

et al. 2020

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“…The action of H 2 O 2 on the structure and conformation of hemoglobin is well documented. 58,59 Hemoglobin is particularly sensitive to oxidation, which irreversibly changes its structure. A solution of 30% H 2 O 2 was therefore employed to treat the samples for 5 min, aer which the samples were washed in 70% ethanol and 100% industrial methylated spirits (see Material and methods for full description of the protocol).…”

Section: Dealing With the Pattern 2: Reversion To Patternmentioning

confidence: 99%

Processing ThinPrep cervical cytological samples for Raman spectroscopic analysis

et al. 2014

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“…The separation and analysis of proteins are important in the area of bioscience. CE–CL system has been applied for hemoprotein, enzyme, and tumor marker protein analysis .…”

Section: Applicationsmentioning

confidence: 99%

“…Cheng's group applied CE–CL detection system to detect hemoglobin and released free iron ion (Fe 3+ ) and then monitored the course of hemoglobin reacting with H 2 O 2 , based on the fact that the reaction of hemoglobin with H 2 O 2 led to fluorescent product and heme degradation. They discovered an intermediate product of heme degradation during the reaction course, which intensely enhanced the luminol–H 2 O 2 CL reaction.…”

Section: Applicationsmentioning

confidence: 99%

Recent advances in chemiluminescence detection coupled with capillary electrophoresis and microchip capillary electrophoresis

2015

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CE is an ideal analytical method for extremely volume-limited biological microenvironments. However, the small injection volume makes it a challenge to achieve highly sensitive detection. Chemiluminescence (CL) detection is characterized by providing low background with excellent sensitivity because of requiring no light source. The coupling of CL with CE and MCE has become a powerful analytical method. So far, this method has been widely applied to chemical analysis, bioassay, drug analysis, and environment analysis. In this review, we first introduce some developments for CE-CL and MCE-CL systems, and then put the emphasis on the applications in the last 10 years. Finally, we discuss the future prospects.

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Monitoring the reaction of hemoglobin with hydrogen peroxide by capillary electrophoresis-chemiluminescence detection

Cited by 13 publications

References 32 publications

Ultrasensitive haptoglobin biomarker detection based on amplified chemiluminescence of magnetite nanoparticles

Ultrasensitive haptoglobin biomarker detection based on amplified chemiluminescence of magnetite nanoparticles

Processing ThinPrep cervical cytological samples for Raman spectroscopic analysis

Recent advances in chemiluminescence detection coupled with capillary electrophoresis and microchip capillary electrophoresis

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