Monitoring the battleground: exploring antimicrobial resistance and virulence factors in wound bacterial isolates

Awuor, Silas Onyango; Omwenga, Eric O.; Mariita, Richard M.; Musila, Jonathan M.; Musyoki, Stanslaus

doi:10.1099/acmi.0.000613.v6

Cited by 2 publications

(1 citation statement)

References 31 publications

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“…During the last decade, the family Enterobacteriaceae was considered the main cause of nosocomial infections due to their ubiquity and resistance profile ( 2 ). Some members of this family are included in the ESKAPE group ( Enterococcus , S. aureus , K. pneumoniae , A. baumannii , P. aeruginosa , and E. coli ) and can cause economic impact ( 3 , 4 ), and increase healthcare institutions’ burden ( 5 , 6 ). Monitoring the microbiota of ICU patients using active surveillance cultures (ASCs) could prevent the occurrence of HAIs by early identification of colonized or infected patients.…”

Section: Introductionmentioning

confidence: 99%

Association between multidrug-resistant bacteria and outcomes in intensive care unit patients: a non-interventional study

Martins,

da Mata,

dos Santos

et al. 2024

Front. Public Health

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BackgroundIn intensive care units (ICUs), infections by multidrug-resistant (MDR) microorganisms should be monitored to prevent healthcare-associated infections (HAIs).MethodsFrom 2018 to 2020, we investigated all medical records of patients admitted to the ICU of a public university hospital. All patients colonized/infected by MDR microorganisms and submitted to active surveillance cultures (ASCs) were included.Results and discussionMale patients prevailed, and 9.5% were positive for MDR bacteria. In-hospital deaths were statistically significant (p < 0.05) for older patients, patients with orotracheal tube use during previous and current hospitalization, and patients with high blood pressure, cardiac and pulmonary diseases, and chronic kidney disease. Carbapenem resistant Enterobacteriaceae was the most frequently resistance profile, followed by extended-spectrum beta-lactamase. The diagnosis or evolution of HAIs was statistically significant (p < 0.0001) for patients treated with meropenem and vancomycin, and in-hospital deaths occurred in 29.5% of patients using polypeptides while the use of macrolides reduced the odds for mortality. The BRADEN Scale demonstrated that 50% of the patients were at high risk of dying.ConclusionPatients hospitalized in the ICU, colonized or infected by MDR bacteria, using invasive medical devices, and with underlying medical conditions presented increased mortality rates. The prescription of meropenem and vancomycin should be carefully monitored once patients using these antimicrobials already have or develop an HAI.

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Section: Introductionmentioning

confidence: 99%

Association between multidrug-resistant bacteria and outcomes in intensive care unit patients: a non-interventional study

Martins,

da Mata,

dos Santos

et al. 2024

Front. Public Health

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Inter-Species Competition of Mono- or Dual Species Biofilms- of MDR-Staphylococcus aureus and Pseudomonas aeruginosa Promotes the Killing Efficacy of Phage or Phage Cocktail

RojaRani,

Reddy,

Vijayalakshmi

et al. 2024

Applied Microbiology

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Pseudomonas aeruginosa and Staphylococcus aureus are opportunistic bacteria frequently linked to burn wound infections. These bacteria can grow as biofilms, which increases their level of drug resistance to current antibiotics. The purpose of the present study is to analyze the effect of biofilm formation, phage and phage cocktail action on single species and dual species biofilms I, e the coexistence of Gram positive (S. aureus) and Gram negative (P. aeruginosa). To this scenario, we employed multi-drug resistant bacteria (P. aeruginosa and S. aureus at 109 CFU/µL) biofilm as single and in combination of both Gram-positive and Gram-negative bacterial biofilms of 24 h grown with respective phage (109 PFU/µL) and phage cocktail (109 PFU/µL) at 4 h of incubation under static conditions. The bacteriolytic activity of phages vB_SAnS_SADP1 and vB_PAnP_PADP4 on 24-h-old biofilms of P. aeruginosa (0.761 ± 0.031) and S. aureus (0.856 ± 0.055), both alone and in combination (0.67 ± 0.02), was the focus of this investigation. The structural organization of biofilms in single- or dual-species combinations under in vitro conditions was validated by scanning and confocal laser scanning microscopy investigations. After 24 h of incubation, single-species biofilms are denser and more resilient whereas dual species biofilms are more loosely associated. Loose association of dual-species biofilm under scanning electron microscopic images at the same conditions, indicated the interspecies -competition of the Gram-positive and Gram-negative bacteria and dual-species biofilms (0.67 ± 0.02) have weak associations and are readily impacted by phage and a phage cocktail (0.16 ± 0.02). Dual-species biofilms were more readily impacted in in vitro settings.

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Monitoring the battleground: exploring antimicrobial resistance and virulence factors in wound bacterial isolates

Cited by 2 publications

References 31 publications

Association between multidrug-resistant bacteria and outcomes in intensive care unit patients: a non-interventional study

Association between multidrug-resistant bacteria and outcomes in intensive care unit patients: a non-interventional study

Inter-Species Competition of Mono- or Dual Species Biofilms- of MDR-Staphylococcus aureus and Pseudomonas aeruginosa Promotes the Killing Efficacy of Phage or Phage Cocktail

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