Monitoring ¹¹¹In-labelled polyisocyanopeptide (PIC) hydrogel wound dressings in full-thickness wounds

Abstract

Applying PIC hydrogel wound dressings functionalized and labelled with 111In-DTPA to skin wounds allows monitoring of biodistribution with SPECT/CT.

Cell-matrix reciprocity in 3D culture models with nonlinear elasticity

“…The gels are fully biocompatible, also in in vivo settings. Beyond their applications as in vitro cell culture matrices, PIC gels are considered as artificial cytoskeleton models [ 121 ], for 3D printing [ 122 ], in antimicrobial assays [ [123] , [124] , [125] ], and for wound healing [ [126] , [127] , [128] ], immunological [ 129 ] and periodontal applications [ 130 , 131 ].…”

“…The investigation on the long-term degradability of PIC is still in process, although several in vivo reports find that PIC gels do not degrade readily within several weeks to months [ 126 , 127 ]. This raises the question: can cells remodel the PIC matrix to initiate the typical responses of its natural collagen or fibrin counterparts?…”

Semiflexible polymer scaffolds: an overview of conjugation strategies

“…Wagener and colleagues demonstrated its use as a washable dressing for abdominal wounds. 252,253 Yang and coworkers developed an injectable (at 5 °C) and in situ gel forming composite with Lipoxin A 4containing poly(lactic-co-glycolic acid) (PLGA) microparticles and found sustained release in treatment against periodontitis in animal studies. 254,255 Xing and coworkers used the scaffolding capacity of the PICP backbone to induce conformational changes in water-soluble conducting polymers (polythiophenes and polyfluorenes).…”

“…262,263 The materials have been used in a number of in vitro 3D cell culture studies, where the readily controlled (mechanical) properties were used to tailor cell behavior, for instance towards stem cell differentiation 263,264 and secretome modification, 265 , cancer therapy, 266 morphogenesis, 267 organoid formation, 268,269 vascularization 270 and T-cell expansion. 271 Using the same SPAAC approach other functional groups, such as dyes 248 or PET probes 253 have been introduced. To tune the mechanical behavior and the thermal response of the wsPICPs, crosslinks were introduced via the same SPAAC approach.…”

Recent Advances in Bioorthogonal Click Chemistry for Efficient Synthesis of Radiotracers and Radiopharmaceuticals

“…Next, PIC-DTPA was reacted with 111 InCl 3 to give the 111 In-labeled PIC in high RCY. The radiolabeled PIC was applied in a SPECT imaging study for evaluating the efficacy of PIC gels in wound mouse models [74]. Figure 8 shows the 99m Tc labeling of human serum albumin (HSA) via a SPAAC reaction.…”