Monitoring Spore Dispersal and Early Infections of <i>Diplocarpon coronariae</i> Causing Apple Blotch Using Spore Traps and a New qPCR Method

Boutry, Clémence; Bohr, Anne; Buchleither, S.; Ludwig, Mathias; Oberhänsli, Thomas; Tamm, Lucius; Schärer, Hans-Jakob; Flury, Pascale

doi:10.1094/phyto-05-22-0183-r

Cited by 2 publications

(1 citation statement)

References 43 publications

(49 reference statements)

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“…All templates that did not amplify for one of the newly designed primer-probe sets were tested with ITS1-F/ITS4 (Colletotrichum, Neonectria [53,54], Dc_09_F/Dc_09_R (Marssonina [55]) or COX-F/COX-R primers (plants [30]) to confirm that the DNA had no PCR inhibitors.…”

Section: Introductionmentioning

confidence: 99%

New Species-Specific Real-Time PCR Assays for Colletotrichum Species Causing Bitter Rot of Apple

McHenry,

Aćimović

2024

Microorganisms

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Bitter rot of apple is an economically important worldwide disease caused by different Colletotrichum species, depending on many factors such as climate, geography, other hosts, and crop management practices. Culture, morphology, and single-locus sequencing-based methods for identifying the Colletotrichum species are severely limited in effectiveness, while the multilocus sequence typing methods available for delineating species are costly, time-intensive, and require high expertise. We developed species-specific hydrolysis probe real-time PCR assays for the following nine Colletotrichum species causing bitter rot in the Mid-Atlantic U.S.A.: C. fructicola, C. chrysophilum, C. noveboracense, C. gloeosporioides s.s., C. henanense, C. siamense and C. theobromicola from the C. gloeosporioides species complex, and C. fioriniae and C. nymphaeae from the C. acutatum species complex. After searching 14 gene regions, we designed primers and probes in 5 of them for the nine target species. Four primer–probe set pairs were able to be duplexed. Sensitivity tests showed as little as 0.5 pg DNA were detectable. These real-time PCR assays will provide rapid and reliable identification of these key Colletotrichum species and will be critically important for studies aiming to elucidate their biology, epidemiology, and management on apples as the number one produced and consumed tree fruit in the U.S.A.

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Section: Introductionmentioning

confidence: 99%

New Species-Specific Real-Time PCR Assays for Colletotrichum Species Causing Bitter Rot of Apple

McHenry,

Aćimović

2024

Microorganisms

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An Overview of Pest and Disease Occurrence in Organic Pome Fruit Orchards in Europe and on the Implementation of Practices for Their Control

et al. 2022

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There is limited data regarding the specific problems faced by organic fruit growers when dealing with plant protection, particularly at a European Union level, though some general knowledge about pest and disease incidence can be found. Such information is crucial to improve the efficacy of a targeted knowledge transfer to organic fruit growers and advisors aiming at an increased adoption of innovative practices. A survey was thus carried out in seventeen European countries (16 EU member states and Switzerland), within the framework of the EU-funded project BIOFRUITNET, aiming at filling this knowledge gap also in terms of research needs. A questionnaire including a section about general aspects of orchard management (functional biodiversity, fertilization management, varietal/rootstock selection) and a section specifically dedicated to pest and disease occurrence and management in organic orchards was utilized to interview about 250 professionals (farmers and advisors), 155 of which were involved in pome fruits (including apple and pear) production. The analysis of the answers related to plant protection pointed out a varied situation about pest and disease occurrence in apple and pear orchards across Europe, though related to the zonal location of the respondent. However, more than 50% of respondents generally considered just few among the most damaging ones, normally co-occurring in the orchards. Interestingly, regardless of the respondents’ nationality or zonal location, more pests than diseases were indicated as relevant agents threatening organic pome fruits production. Nevertheless, only few measures promoting functional biodiversity in the orchards resulted in being broadly implemented in all regions. The analysis of the data underlines the strong demand for the development of a toolbox of measures that can be integrated successfully into the general orchard management strategy including the successful enhancement of functional or general biodiversity.

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Monitoring Spore Dispersal and Early Infections of Diplocarpon coronariae Causing Apple Blotch Using Spore Traps and a New qPCR Method

Cited by 2 publications

References 43 publications

New Species-Specific Real-Time PCR Assays for Colletotrichum Species Causing Bitter Rot of Apple

New Species-Specific Real-Time PCR Assays for Colletotrichum Species Causing Bitter Rot of Apple

An Overview of Pest and Disease Occurrence in Organic Pome Fruit Orchards in Europe and on the Implementation of Practices for Their Control

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