Monitoring Simulated Bioflocculation: Application of Micro-Flow Imaging Technology

Kollu, Kerim; Örmeci, Banu

doi:10.1007/s11270-015-2299-4

Cited by 2 publications

(1 citation statement)

References 23 publications

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“…Object intensity measures the light that reaches the detector after passing through the object, and the closer the value is to 255, the more porous the particle is (Kollu and Örmeci, 2015). Object intensities are described in three formats, minimum and maximum intensities correspond to the lowest and highest intensities, while the mean refers to the average of all pixel intensities.…”

Section: Object Intensitymentioning

confidence: 99%

Micro-flow imaging for in-situ and real-time enumeration and identification of microplastics in water

Choran

Örmeci

2023

Front. Water

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Microplastics (MPs) are emerging contaminants that have recently gained global attention. Current identification and quantification methods are known to be time-consuming, labor-intensive, and lack consensus on protocol standardization. This study explored the potential of micro-flow imaging (MFI) technology for rapid and in-situ identification and enumeration of MPs in water using two (2) MFI-based particle counters. Advantages, limitations, and recommendations for using MFI for MPs analysis were discussed. MPs with diverse physical (i.e., microbeads, fragments, fibers, and films) and surface (i.e., reflectivity, microporosity, color) characteristics were analyzed to understand the detection capabilities and limitations of MFI technology. Results demonstrated that MFI effectively automates most manually obtained particle features, such as size, color, object intensity and shape descriptors. It imparts consistency and reduces the subjective nature of results, thus enabling reliable comparison of the generated data. The particles can be further categorized based on their circularity and aspect ratio providing further insight into the shape and potential erosion of MPs in the environment. Transparent particles, often missed with other techniques such as microscopy, were detected by the MFI technology. The ability to assign particle IDs to MPs was an important advantage of the MFI technology that enabled the further investigation of selected MPs of interest. The limitations of the MFI technology were apparent in samples with high particle concentrations, with reflective MPs, and in the presence of bubbles. The color of the background against which the image was captured also influenced the detection accuracy. Procedural modifications during sample analysis and improvements in image analysis can assist in overcoming these challenges. MFI requires minimal sample preparation and gives real-time imaging data, making it a prime candidate for field monitoring in surface water systems in addition to laboratory analysis. With the potential application of machine learning and similar developments in the future, MFI-based particle counters are well-positioned to meet an important need in in-flow and real-time identification and enumeration of MPs.

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Section: Object Intensitymentioning

confidence: 99%

Micro-flow imaging for in-situ and real-time enumeration and identification of microplastics in water

Choran

Örmeci

2023

Front. Water

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Chlorination caused a shift in marine biofilm niches on microfiltration/ultrafiltration and reverse osmosis membranes and UV irradiation effectively inactivated a chlorine-resistant bacterium

Cho

Jeong

Lee

et al. 2018

Appl Microbiol Biotechnol

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The effect of chlorine disinfection on marine biofilm populations and communities formed on membrane surfaces was investigated under two feedwater conditions: raw seawater and deep bed filtration-treated seawater. As a result of chlorination, the structure of the biofilm community on the microfiltration/ultrafiltration and reverse osmosis membrane coupons shifted significantly at the genus level. However, the total bacterial population was not reduced under the two feedwater conditions. This failure to control the biofilm was attributed to the adaptation and survival of selected bacteria under chlorine stress. Phaeobacter caeruleus, isolated from the biofilm, was examined as a representative chlorine-resistant biofilm-forming bacterium. The number of viable P. caeruleus was significantly reduced (as much as 99.8%) after ultraviolet (UV) disinfection. The results indicated that additional disinfection by UV irradiation can inactivate chlorine-resistant bacteria. Therefore, tandem chlorination-UV disinfection may enhance the efficiency of biofouling control in seawater reverse osmosis processes. The synergistic effects of tandem chlorination-UV irradiation on the marine biofilm community should be investigated in future studies.

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Monitoring Simulated Bioflocculation: Application of Micro-Flow Imaging Technology

Cited by 2 publications

References 23 publications

Micro-flow imaging for in-situ and real-time enumeration and identification of microplastics in water

Micro-flow imaging for in-situ and real-time enumeration and identification of microplastics in water

Chlorination caused a shift in marine biofilm niches on microfiltration/ultrafiltration and reverse osmosis membranes and UV irradiation effectively inactivated a chlorine-resistant bacterium

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