Monitoring of the Viability of Cells Immobilized by Sol-Gel Process

Bioluminescence of Pseudomonas fluorescens HK44 in the course of encapsulation into silica gel. Effect of methanol

“…For immobilization procedure and composition of the biofilms see Kuncová et al (2004) and Trögl et al (2005). Si(OMe) 4 (Fluka no.…”

“…The negative effect of the evolving alcohols was described (Ingram and Buttke 1984;Coiffier et al 2001;Desimone et al 2002;Ferrer et al 2003;Livage and Coradin 2006) and non-alcoholic routes that eliminate alcohol before the admixture of cells were developed (Fennouh et al 2000;Nassif et al 2003;Ferrer et al 2006). Nevertheless, high survival rates as well as long-term viability of immobilized cells were achieved when cells were encapsulated by alcoholic routes (Kuncová et al 2002(Kuncová et al , 2004Trögl et al 2005;Mitchel and Gu 2006;Gavlasová et al 2008;Kuppard et al 2009). The advantages of alcoholic routes include the ease of preparation, better toughness, mechanical durability and transparency (Adamová 2009;Kuncová and Trögl 2010).…”

“…Changes in light emission or the production of fluorescent compounds by microorganisms might also be used as signals of unspecified stress (Kuncová et al 2004;Tessema et al 2006). However, other factors affecting BLM such as growth-phase (Heitzer et al 1992) or membrane perturbations (Heitzer et al 1998;Trögl et al 2007Trögl et al , 2010) have yet to be taken into account.…”

Silica hybrid nanocomposites

“…One of the fastest growing research directions in the field of sol-gel science and technology is the immobilization of various biomolecules in sol-gel matrices retaining their bioactivity. A number of enzymes, antibodies and cells have been successfully immobilized in silica and organic-inorganic materials [17][18][19][20][21][22][23][24][25][26][27]. The porous structure of the hybrids is a major parameter for the efficiency of the immobilization process as the pores of the hybrid materials having nano dimensions helping the diffusion of the substrates and products of the enzyme reaction.…”

“…The main function of core materials is to entrap biomolecules and living cells rapidly whilst maintaining their biological activity [24][25][26][27][28].…”