Monitoring of the enzymatic activity of intracellular lipases of <em>Ustilago maydis</em> expressed during the growth under nitrogen limitation and its correlation in lipolytic reactions
Abstract:Under nitrogen starvation, Ustilago maydis forms lipid droplets (LDs). Although the dynamics of these organelles are known in the literature, the identity of the lipases implicated in their degradation is unknown. We determined lipase activity and identified the intracellular lipases expressed during growth under nitrogen starvation and YPD media by zymograms. The results showed that cytosolic extracts exhibited higher lipase activity when cells were grown in YPD. Under nitrogen starvation, lipase activity was… Show more
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