Monitoring of Propofol Boli in Breathing Gas using Ion Molecule Reaction Mass Spectrometry

Varadarajan, B.; Großherr, M.; Meyer, Jan; Dibbelt, L.; Gehring, Hartmut; Hengstenberg, Andreas

doi:10.1007/978-3-642-03885-3_119

Cited by 1 publication

(1 citation statement)

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“…Based on the apolar and considerable volatile character of propofol, polydimethylsiloxane was the fiber material of choice for DI‐SPME . Method development included the design of a reproducible preconcentration step followed by thermal desorption in the GC injector system.…”

Section: Resultsmentioning

confidence: 99%

Development of a highly sensitive gas chromatography–mass spectrometry method preceded by solid‐phase microextraction for the analysis of propofol in low‐volume cerebral microdialysate samples

Guntner

Stöcklegger

Kneidinger

et al. 2019

J of Separation Science

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To date, the commonly used intravenous anesthetic propofol has been widely studied, and fundamental pharmacodynamic and pharmacokinetic characteristics of the drug are known. However, propofol has not yet been quantified in vivo in the target organ, the human brain. Here, cerebral microdialysis offers the unique opportunity to sample propofol in the living human organism. Therefore, a highly sensitive analytical method for propofol quantitation in small sample volumes of 30 μL, based on direct immersion solid‐phase microextraction was developed. Preconcentration was followed by gas chromatographic separation and mass spectrometric detection of the compound. This optimized method provided a linear range between the lower limit of detection (50 ng/L) and 200 μg/L. Matrix‐matched calibration was used to compensate recovery issues. A precision of 2.7% relative standard deviation between five consecutive measurements and an interday precision of 6.4% relative standard deviation could be achieved. Furthermore, the permeability of propofol through a cerebral microdialysate system was tested. In summary, the developed method to analyze cerebral microdialysate samples, allows the in vivo quantitation of propofol in the living human brain. Additionally the calculation of extracellular fluid levels is enabled since the recovery of the cerebral microdialysis regarding propofol was determined.

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Section: Resultsmentioning

confidence: 99%