Monitoring of patients with nonseminomatous testicular cancer by nuclear magnetic resonance spectroscopy of plasma

Höfeler, H.; Scheulen, M. E.

doi:10.1016/0277-5379(89)90406-9

Cited by 7 publications

(2 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similar results which show that ROC analysis is of little diagnostic value have also been obtained by others (Otvos et al, 1991). Two further reports (Peeling et al, 1988;Hofeler & Scheulen, 1989) credit in vitro NMR spectroscopy with a greater capacity to detect cancer but they still found a considerable overlap between the various patient groups (Peeling et al, 1988), and inconsistent results when the experiment was repeated (Hofeler & Scheulen, 1989). They did not determine the serum triglyceride levels (Peeling et al, 1988;Hofeler & Scheulen, 1989).…”

Section: Data Collectionmentioning

confidence: 99%

Failure to detect early breast cancer using in vitro nuclear magnetic resonance spectroscopy of plasma

Holmberg

Jakobsson²,

Berglund³

et al. 1993

Br J Cancer

View full text Add to dashboard Cite

Summary Water suppressed proton nuclear magnetic resonance ('H NMR) spectroscopy of human plasma has been described as successful in detection of malignancy. We designed a prospective study to test the hypothesis that in vitro NMR spectroscopy has a high sensitivity for detecting early breast cancer. One hundred and thirty-five women were referred for breast biopsy due to abnormal mammograms. One hundred of these were recruited through a population-based mammography screening project. Sixty-nine of 135 women were found to have breast cancer and their average line width of the methyl and methylene resonance in the plasma were compared to those women who had a benign or normal histopathology in the biopsy and to the line width for 100 healthy subjects from the same population. Thus the biopsy group was subdivided into one group with malignant and another with benign or normal histological findings. The sample size was predetermined to 120 by power calculations. Our aim was to detect differences in line width of the same magnitude as that described by Fossel, with 80% power and a 5% significance level. One hundred of the women were referred directly from a mammography screening center. The screening was a population-based public health care program for mammography alone.To obtain a reference group that was not subject to preoperative psychological stress, 100 healthy subjects from the same population were enrolled from an ongoing populationbased screening study for primary hyperparathyroidism among women 50 years of age and older. All these women gave their informed consent. Data collectionOn the day before surgery the women in the biopsy group were asked whether they had had a malignant tumour earlier in life, whether they smoked, whether they were on a special diet, when they had had their most recent meal and what it consisted of. Blood samples for determinations of the line width, serum triglycerides, serum cholesterol, serum FSH and estradiol were then drawn. Data about source of referral, histopathological diagnosis and tumour stage were obtained from the medical records.The healthy women were asked the same set of questions as those in the biopsy group. At the same time as the blood samples were drawn for the primary hyperparathyroidism screening, a blood sample for line width, serum triglycerides and serum cholesterol was drawn.A second blood sample was taken from 20 of the 69 patients with breast cancer 3-4 months after completion of therapy. These women had stage I or II cancer and were considered to be free of disease at the time.Laboratory methods EDTA-prepared tubes were used for blood sampling. The samples were centrifuged to obtain the plasma fraction within 60 min.

show abstract

Section: Data Collectionmentioning

confidence: 99%

Failure to detect early breast cancer using in vitro nuclear magnetic resonance spectroscopy of plasma

Holmberg

Jakobsson²,

Berglund³

et al. 1993

Br J Cancer

View full text Add to dashboard Cite

show abstract

“…A significant correlation between the average linewidth of the methylene and methyl composite resonances with the presence or absence of malignancy was demonstrated (p< 0.0001). Since the publication of that report, we and others have further investigated this NMR application in various clinical settings [2][3][4][5][6][7][8][9][10][11][12][13][14].…”

Section: Introductionmentioning

confidence: 99%

C-13 NMR spectroscopy of plasma reduces interference of hypertriglyceridemia in the H-1 NMR detection of malignancy. Application in patients with breast lesions

Fossel

Hall

McDonagh³

1991

Breast Cancer Res Tr

View full text Add to dashboard Cite

We have previously described the application of water-suppressed proton nuclear magnetic resonance (H-1 NMR) spectroscopy of plasma for detection of malignancy. Subsequently, hypertriglyceridemia has been identified as a source of false positive results. We now describe a confirmatory, adjunctive technique--analysis of the carbon-13 (C-13) NMR spectrum of plasma--which also identifies the presence of malignancy but is not sensitive to the plasma triglyceride level. Blinded plasma samples from 480 normal donors and 208 patients scheduled for breast biopsy were analyzed by water-suppressed H-1 and C-13 NMR spectroscopy. Triglyceride levels were also measured. Among the normal donors, there were 38 individuals with hypertriglyceridemia of whom 18 had results consistent with malignancy by H-1 NMR spectroscopy. However, the C-13 technique reduced the apparent H-1 false positive rate from 7.0% to 0.6%. Similarly, in the breast biopsy cohort, C-13 reduced the false positive rate from 2.8% to 0.9%. Furthermore, the accuracy of the combined H-1/C-13 test in this blinded study was greater than 96% in 208 patients studied.

show abstract

Nuclear magnetic resonance spectroscopy of plasma to distinguish between malignant and benign diseases causing jaundice and cholestasis

Pasanen

Kauppinen

Eskelinen

et al. 1993

J Cancer Res Clin Oncol

View full text Add to dashboard Cite

The sera of 51 patients with malignant (n = 25) and benign (n = 26) hepatopancreatobiliary disorders were analysed by 1H magnetic resonance spectroscopy (NMR) in order to distinguish between malignant and benign diseases causing jaundice and/or cholestasis. Macromolecular linewidths were determined both manually and automatically with a computed analysis, and both methylene (CH2) and methyl (CH3) resonances were evaluated. The mean linewidth of the CH3 peak was significantly narrower in the patients with malignant disease than in the patients with benign disease both in the manual and computed analyses, but no significant differences in the CH2 peak were detected. Diagnostic sensitivity and specificity of the CH3 peak determined in the computed analysis were 92% and 27% respectively. In the light of the current study, it seems obvious that because overlap between benign and malignant groups was too great, 1H NMR spectroscopy of plasma is not of practical value in distinguishing between benign and malignant causes of jaundice and/or cholestasis.

show abstract

Monitoring of patients with nonseminomatous testicular cancer by nuclear magnetic resonance spectroscopy of plasma

Cited by 7 publications

References 7 publications

Failure to detect early breast cancer using in vitro nuclear magnetic resonance spectroscopy of plasma

Failure to detect early breast cancer using in vitro nuclear magnetic resonance spectroscopy of plasma

C-13 NMR spectroscopy of plasma reduces interference of hypertriglyceridemia in the H-1 NMR detection of malignancy. Application in patients with breast lesions

Nuclear magnetic resonance spectroscopy of plasma to distinguish between malignant and benign diseases causing jaundice and cholestasis

Contact Info

Product

Resources

About