Monitoring of iron(III) removal from biological sources using a fluorescent siderophore

A Review of Fluorescence Methods for Assessing Labile Iron in Cells and Biological Fluids

“…(b) Under ambient conditions, the predicted probe: iron-binding stoichiometry should be 1:1 for FL-DFO and (36), and FL-deferrioxamine (FL-DFO, DFO-Green) by reacting fluorescein-5-isothiocyanate with DFO, while NBD-DFO is obtained by reacting NBD-CI with DFO (34). Calcein is composed of FL conjugated with an EDTA-like moiety (30).…”

“…In Fig. 1 is shown the first generation of probes designed to meet the metal-sensor criteria, as represented by NBD and FL analogs of deferrioxamine (NBD-DFO and FL-DFO) (30,34), reversed siderophores (RSF) (35), DTPA (FL-DTPA) (36), EDTA (calcein, CAL), phenanthroline (FL-phen, Phen Green), and FL specifically tagged onto transferrin (37) (FL-Tf). Other natural or synthetic siderophores, distinct from DFO, have been either coupled to fluorescent probes or rendered intrinsically fluorescent in a pursuit for novel iron-specific fluorescent probes (35, 38 -42).…”

Desferrioxamine-chelatable iron, a component of serum non–transferrin-bound iron, used for assessing chelation therapy

“…12 The use of the fluorescent Fe-responsive probe allowed us to directly verify the existence of a DFO-chelatable component of NTBI. In addition to determining DCI in serum, the assay enabled us to follow this serum Fe fraction during chelator therapy with deferriprone (L1) and DFO.…”

Recognition of iron ions by carbazole–desferrioxamine fluorescent sensor and its application in total iron detection in airbone particulate matter

“…Recently, many oriented iron fluorescent probes have been developed [18,19] with the use of crown ether supramolecular framework [20][21][22] or highly iron selective chelating molecules such as natural [23,24] and synthetic siderophore analogs [25][26][27], which form an important class of compound whose primary function is to mediate iron uptake by microbial cells. Thus, on the basis of the large chemical affinity for Fe(III) relative to other diand trivalent metals [28], the desferrioxamine B (DFO-B) is an extensively studied siderophore.…”