Monitoring of Irinotecan in Human Plasma: Sensitive Fluorescent Nanogels by Molecular Imprinting

Abstract

A series of fluorescent molecularly imprinted nanogels to detect irinotecan (CPT11) were prepared and characterized. A set of amino acids and napthalimide polymerisable derivatives allowed to obtain polymers as soluble fluorescent nanoparticles by high dilution imprinted synthesis. The direct detection of irinotecan in human plasma was obtained by fluorescence quenching of the naphtalimide-based imprinted materials. The plasma sample treated with acetonitrile allowed the detection of irinotecan in the 10nM – 30μM range. The LOD was 9.4 nM, with within-run variability 10% and day to day variability 13%.<br>

Signal-On Fluorescent Imprinted Nanoparticles for Sensing of Phenols in Aqueous Olive Leaves Extracts

“…With this purpose in mind, different monomers, co-monomers and cross-linkers have been tested in order to achieve polymers with the optimal properties to establish the right equilibrium between the release and capture of the templates. As a first attempt, commercial monomers 5 – 9 were used in the MIPs synthesis, choosing N -isopropylacrylamide (NIPAM) as the co-monomer and N,N′ -methylenebisacrylamide (MBA) as the cross-linker as they showed good results in our previous works [ 18 , 19 ] ( Figure 1 ). Taking advantage of the higher sensitivity and selectivity in general of fluorescent MIPs, the fluorescent co-monomer 13 and 14 , obtained by a literature synthetic procedure, were used.…”