Monitoring of Hemodynamics in Human Skin Using Pulsed Photothermal Radiometry and Optical Spectroscopy

Verdel, Nina; Majaron, Boris

doi:10.21611/qirt.2018.043

Cited by 2 publications

(2 citation statements)

References 13 publications

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“…Using the analysis approach very similar to that presented in current study, we have recently replicated these effects by applying incrementally higher cuff pressures (i.e., 80, 100 and 120 mm Hg) to induce either venous or arterial (a.k.a. total) occlusion [48].…”

Section: Discussionmentioning

confidence: 99%

Physiological and structural characterization of human skin in vivo using combined photothermal radiometry and diffuse reflectance spectroscopy

Verdel¹,

Marin²,

Milanič³

et al. 2019

Biomed. Opt. Express

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In this proof-of-concept study we combine two optical techniques to enable assessment of structure and composition of human skin in vivo: Pulsed photothermal radiometry (PPTR), which involves measurements of transient dynamics in mid-infrared emission from sample surface after exposure to a light pulse, and diffuse reflectance spectroscopy (DRS) in visible part of the spectrum. The analysis involves simultaneous fitting of measured PPTR signals and DRS with corresponding predictions of a Monte Carlo model of light-tissue interaction. By using a four-layer optical model of skin we obtain a good match between the experimental and model data when scattering properties of the epidermis and dermis are also optimized on an individual basis. The assessed parameter values correlate well with literature data and demonstrate the expected trends in controlled tests involving temporary obstruction of peripheral blood circulation using a pressure cuff, and acute as well as seasonal sun tanning.

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Section: Discussionmentioning

confidence: 99%

Physiological and structural characterization of human skin in vivo using combined photothermal radiometry and diffuse reflectance spectroscopy

Verdel¹,

Marin²,

Milanič³

et al. 2019

Biomed. Opt. Express

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show abstract

“…Such quadratic binning was formerly proven to significantly speed up photothermal temperature profiling and related applications with no adverse effects on the quality of the results [5,[11][12][13]17,18]. Namely, in contrast with uniform binning to a similar number of frames, quadratic binning maintains the high frame rate of the original record at early acquisition times, which enables reconstruction of superficial objects with high axial resolution [6][7][8].…”

Section: Pre-processing Of the Radiometric Recordmentioning

confidence: 99%

Photothermal tomographic imaging of optically absorbing inclusions in human skin in vivo

Arh,

Klopčič,

Cvetko

et al. 2024

Photonics in Dermatology and Plastic Surgery 2024

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The concept of photothermal tomography (PTT) involves spatially and temporally resolved detection of blackbody emission from the sample surface after irradiation with a short light pulse. In principle, this allows reconstruction of the light-induced temperature field inside the sample, thus enabling three-dimensional imaging of absorbing structures in strongly scattering biological tissues and organs. However, development of accurate and robust PTT methodology has proven difficult due to the large size and severe ill-posedness of the underlying inverse problem, aggravated by the inherently low signal-to-noise ratios of active infrared radiometry.We discuss here our recently developed PTT system and its first application to human skin in vivo. The experimental setup involves a medical-grade laser emitting milisecond pulses at 532 nm, and a fast mid-infrared camera equiped with a microscope objective. A custom code written in Python is used to reconstruct three-dimensional images of the absorbing structures by performing iterative multidimensional minimization of the difference between the analytically predicted and experimental radiometric record, using a projected -method algorithm. The described approach produces a rather sharp and high-contrast tomographic image of a tattoo layer in a human volunteer's skin with the onset depth of ~0.15 mm. No evident artifacts or even noise appear elsewhere in the reconstructed volume. Applying quadratic binning of the radiometric record significantly reduced the computational load, enabling us to reconstruct a volume of 5.6 x 2.8 x 0.9 mm 3 with nominal resolution of 25 x 25 x 15 m 3 using a personal computer in less than one minute.

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Monitoring of Hemodynamics in Human Skin Using Pulsed Photothermal Radiometry and Optical Spectroscopy

Cited by 2 publications

References 13 publications

Physiological and structural characterization of human skin in vivo using combined photothermal radiometry and diffuse reflectance spectroscopy

Physiological and structural characterization of human skin in vivo using combined photothermal radiometry and diffuse reflectance spectroscopy

Photothermal tomographic imaging of optically absorbing inclusions in human skin in vivo

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