Monitoring of hematopoietic chimerism after sex-mismatched allogeneic stem cell transplantation (alloSCT) by dual-color FISH analysis of X and Y chromosomes

Turkiewicz, Dominik; Gorczyńska, Ewa; Toporski, Jacek; Kałwak, Krzysztof; Rybka, Blanka; Noworolska, Dorota; Bogusławska-Jaworska, J; A, Chybicka

doi:10.1016/s0145-2126(03)00077-8

Cited by 12 publications

(10 citation statements)

References 14 publications

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“…Mainly differences in CD45 (45.1 and 45.2) or Thy 1 (Thy 1.1 and Thy1.2) were used, but also antibodies against H-2 have been described. [7][8][9][10] The standard techniques for determining chimerism in humans are fluorescence in situ hybridization (FISH) of sex chromosomes [11][12][13][14] or PCR of long terminal repeats. [15][16][17] These methods allow sensitive detection of the chimeric proportion of the cells, but give no information on a single cell level.…”

Section: Introductionmentioning

confidence: 99%

Flow cytometry with anti HLA-antibodies: a simple but highly sensitive method for monitoring chimerism and minimal residual disease after HLA-mismatched stem cell transplantation

Schumm

Feuchtinger

Pfeiffer

et al. 2007

Bone Marrow Transplant

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Transplantation of HLA-mismatched stem cells may allow determination of chimerism status of single cells by differential expression of HLA molecules. Monoclonal antibodies against HLA antigens can be used to determine the HLA type of sub-populations by standard flow cytometry. Blood samples from 23 patients transplanted from HLA-mismatched family donors were monitored using HLA-specific antibodies. Suitable antibodies could be found for all donor recipient pairs by using differences in HLA Bw4 and Bw6 groups or other serological antigens. Pretransplant controls of donor and recipient were used to correct for variable fluorescence intensities of the antibodies and sub-populations. Owing to the high sensitivity, cell populations with a minimum frequency of 0.1% were detectable. Flow-cytometric analysis was confirmed by chimerism analysis of immunomagnetically isolated T cells by standard PCR technique. In addition to chimerism evaluation, HLA antibodies improved the detection of leukemic cells after transplantation with aberrant phenotype. In conclusion, flow cytometry using antibodies against HLA antigens is an interesting tool for determination of chimerism and minimal residual disease after HLA-mismatched transplantation. Information about the chimerism status is given on a single-cell level and allows fast and convenient analysis of sub-populations.

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Section: Introductionmentioning

confidence: 99%

Flow cytometry with anti HLA-antibodies: a simple but highly sensitive method for monitoring chimerism and minimal residual disease after HLA-mismatched stem cell transplantation

Schumm

Feuchtinger

Pfeiffer

et al. 2007

Bone Marrow Transplant

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“…Metaphase‐FISH and combined investigation of BCR / ABL and sex chromosome‐specific i‐FISH patterns revealed the explanation for this apparent contradiction. Although several previous studies have concluded that XY‐FISH is a robust method for assessing the chimeric state, and that Y‐QPCR is one of the most sensitive techniques for detecting minimal residual disease in male recipients of female donors 4, 5, 10, 11, it must be noted that sex chromosome changes can confuse the results of chimerism analysis. This fact is particularly relevant in patients with no well characterized, leukemia‐specific marker.…”

Section: Discussionmentioning

confidence: 99%

“…Monitoring of the chimeric state and residual tumor load after allo‐BMT is important 2, 3. In the case of sex‐mismatched allo‐BMT, sex chromosomes are usually used as markers of chimerism based on different cell‐ and DNA‐based techniques 4–6.…”

Section: Introductionmentioning

confidence: 99%

Sex chromosome changes after sex-mismatched allogeneic bone marrow transplantation can mislead the chimerism analysis

Alpár

Nagy

Hohoff³

et al. 2010

Pediatr. Blood Cancer

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A 12-year-old male with pre-B-cell acute lymphoblastic leukemia with cryptic BCR/ABL rearrangement underwent sex-mismatched allogeneic bone marrow transplantation (allo-BMT). Contradictory results were provided by various chimerism analyses 3 months later. Y-chromosome-specific quantitative polymerase chain reaction and sex chromosome-specific interphase fluorescence in situ hybridization (i-FISH) showed complete donor chimerism. Analysis of autosomal short tandem repeats (A-STR), BCR/ABL i-FISH test, and X-STR haplotype indicated relapse. Metaphase-FISH and combined BCR/ABL and sex chromosome-specific i-FISH patterns revealed loss of the Y-chromosome and duplication of the X-chromosome in the host cells. Sex chromosome changes after allo-BMT can cause significant difficulties in chimerism analysis.

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“…It can be used in sexmismatched allogeneic stem cell transplants to detect host and donor cells and to examine the chimaerism. 30 …”

Section: Chimaerismmentioning

confidence: 99%

Stem Cell Through Present and Future

et al. 2009

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Stem cell transplantation (SCT) has the potential to transform the lives of children with a wide variety of genetic diseases, ranging from inherent defects of hemopoietic cell production or function through to metabolic diseases mostly affecting solid organs. For these children life expectancy or quality of life would otherwise be very poor. It ranks as one of the most remarkable therapeutic advances of the past 40 years. Despite rapid technological improvements, however, there are still many short term risks and potential long term toxicities. Consequently, the rapid emergence of alternative therapies (including new drugs, enzyme and gene therapies), necessitate constant re-evaluation of the risk/benefit ratio for each disease and hence the appropriateness of SCT. This review describes the major aspects of the transplant process, indications for transplantation, outcome statistics, and areas where alternative therapies are becoming available. SCT remains a highly experimental therapy. Due to the relatively short history of the discipline no data exists on truly long term follow up. This is important as some organs benefit relatively poorly or problems may emerge which were never apparent as part of the untreated disease. The speed of technological change makes randomised trials on these diseases, which are individually quite rare, almost impossible to perform.

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Monitoring of hematopoietic chimerism after sex-mismatched allogeneic stem cell transplantation (alloSCT) by dual-color FISH analysis of X and Y chromosomes

Cited by 12 publications

References 14 publications

Flow cytometry with anti HLA-antibodies: a simple but highly sensitive method for monitoring chimerism and minimal residual disease after HLA-mismatched stem cell transplantation

Flow cytometry with anti HLA-antibodies: a simple but highly sensitive method for monitoring chimerism and minimal residual disease after HLA-mismatched stem cell transplantation

Sex chromosome changes after sex-mismatched allogeneic bone marrow transplantation can mislead the chimerism analysis

Stem Cell Through Present and Future

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