Monitoring of atrazine treatment on soil bacterial, fungal and atrazine‐degrading communities by quantitative competitive PCR

Martin-Laurent, Fabrice; Piutti, Séverine; Hallet, Stéphanie; Wagschal, Isabelle; Philippot, Laurent; Catroux, Gérard; Soulas, Guy

doi:10.1002/ps.630

Cited by 32 publications

(20 citation statements)

References 40 publications

(42 reference statements)

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“…Previous studies have also shown conflicting results due to the difficulty in establishing the causeand-effect relationship between organic pollutants and enzyme activities (Accinelli et al, 2002;Haney et al, 2002). Atrazine, which is an inhibitor of photosynthesis, has been reported to strongly depress cyanobacteria, to transiently affect the soil fungal microflora and to alter the denitrifying microflora (Isakeit and Lockwood, 1990;Martin-Laurent et al, 2003). In this study bacterial, Gramnegative bacterial, Gram-positive bacterial and arbuscular mycorrhizal fungal community composition showed significant (P < 0.05) atrazine-induced changes.…”

Section: Discussionmentioning

confidence: 99%

Influence of Glomus etunicatum/Zea mays mycorrhiza on atrazine degradation, soil phosphatase and dehydrogenase activities, and soil microbial community structure

Huang

Zhang

et al. 2009

Soil Biology and Biochemistry

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a b s t r a c tThe effects of an arbuscular mycorrhizal (AM) fungus (Glomus etunicatum) on atrazine dissipation, soil phosphatase and dehydrogenase activities and soil microbial community structure were investigated. A compartmented side-arm ('cross-pot') system was used for plant cultivation. Maize was cultivated in the main root compartment and atrazine-contaminated soil was added to the side-arms and between them 650 or 37 mm nylon mesh was inserted which allowed mycorrhizal roots or extraradical mycelium to access atrazine in soil in the side-arms. Mycorrhizal roots and extraradical mycelium increased the degradation of atrazine in soil and modified the soil enzyme activities and total soil phospholipid fatty acids (PLFAs). Atrazine declined more and there was greater stimulation of phosphatase and dehydrogenase activities and total PLFAs in soil in the extraradical mycelium compartment than in the mycorrhizal root compartment when the atrazine addition rate to soil was 5.0 mg kg À1 . Mycelium had a more important influence than mycorrhizal roots on atrazine degradation. However, when the atrazine addition rate was 50.0 mg kg À1 , atrazine declined more in the mycorrhizal root compartment than in the extraradical mycelium compartment, perhaps due to inhibition of bacterial activity and higher toxicity to AM mycelium by atrazine at higher concentration. Soil PLFA profiles indicated that the AM fungus exerted a pronounced effect on soil microbial community structure.

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Section: Discussionmentioning

confidence: 99%

Influence of Glomus etunicatum/Zea mays mycorrhiza on atrazine degradation, soil phosphatase and dehydrogenase activities, and soil microbial community structure

Huang

Zhang

et al. 2009

Soil Biology and Biochemistry

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“…cPCR has been widely used in the medical diagnostics but has also found its application in environmental biotechnology. The use of cPCR with 16s rRNA gene or the biodegradative genes such as ammonia monooxygenase (amoA), atrazine (atzC), cadmium (cadA) as targets have been used in quantification of non-culturable bacteria as well as to assess population density in both pristine and contaminated soil environments (Ka et al 2001;Kurkela et al 1988;Martin-Laurent et al 2003;Oger et al 2001). Mesarch et al (2000) has demonstrated the usefulness of the cPCR for C23DO gene present in contaminated soil samples.…”

Section: Discussionmentioning

confidence: 99%

“…Human activity over the years has led to hydrocarbon contamination of the soils near active and abandoned research stations (Aislabie et al 1999;Martin-Laurent et al 2003;Saul et al 2005). Fuel storage and refueling of aircrafts and other vehicles has led to localized fuel spills on land.…”

Section: Introductionmentioning

confidence: 99%

Detection, expression and quantitation of the biodegradative genes in Antarctic microorganisms using PCR

Panicker

Mojib

Aislabie

et al. 2009

Antonie van Leeuwenhoek

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In this study, 28 hydrocarbon-degrading bacterial isolates from oil-contaminated Antarctic soils were screened for the presence of biodegradative genes such as alkane hydroxylase (alks), the ISPalpha subunit of naphthalene dioxygenase (ndoB), catechol 2,3-dioxygenase (C23DO) and toluene/biphenyl dioxygenase (todC1/bphA1) by using polymerase chain reaction (PCR) methods. All naphthalene degrading bacterial isolates exhibited the presence of a 648 bp amplicon that shared 97% identity to a known ndoB sequence from Pseudomonas putida. Twenty-two out of the twenty-eight isolates screened were positive for one, two or all three different regions of the C23DO gene. For alkane hydroxylase, all 6 Rhodococcus isolates were PCR-positive for a 194 bp and a 552 bp segment of the alkB gene, but exhibited variable results with primers located at different segments of this gene. Three Pseudomonas spp. 4/101, 19/1, 30/3 amplified 552 bp segment of alkB. Only two Pseudomonas sp. 7/156 and 4/101 amplified a segment of alkB exhibiting 89-94% nucleotide sequence identity with the existing sequence of alkB in the GenBank sequence database. Transcripts of three genes, alkB2, C23DO and ndoB, that were amplified by DNA-PCR in three different bacterial isolates also exhibited positive amplification by reverse transcriptase PCR (RT-PCR) method confirming that these genes are functional. A competitive PCR (cPCR) method was developed for a quantitative estimation of ndoB from pure cultures of the naphthalene-degrading Pseudomonas sp. 30/2. A minimum of 1 x 10(7) copies of the ndoB gene was detected based on the comparison of the intensities of the competitor and target DNA bands. It is expected that the identification and characterization of the biodegradative genes will provide a better understanding of the catabolic pathways in Antarctic psychrotolerant bacteria, and thereby help support bioremediation strategies for oil-contaminated Antarctic soils.

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“…To detect the genes involved in the s ‐triazine degradation we used primers specifically designed from conserved regions of the atzA, atzB, atzC, atzD, atzE, atzF and trzN genes (Fig 1). The sequences of these primers and the conditions to run the PCR reactions have been described previously 20, 21…”

Section: Methodsmentioning

confidence: 99%

Degradation of simazine by microorganisms isolated from soils of Spanish olive fields

Santiago-Mora

Martin-Laurent

Prado

et al. 2005

Pest Management Science

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The capability of the microbial flora isolated from an olive field soil from Andalusia to mineralize simazine has been analyzed. From this soil, a group of bacteria capable of degrading 60 mg simazine litre(-1) in less than a week has been isolated. These microorganisms showed a low capacity for degrading this herbicide to carbon dioxide. When total DNA was isolated from this group of bacteria, we were able to detect by PCR the presence of only the atzC and the trzN genes. Some components of this bacterial population have been identified by sequencing of specific fragments from bacterial 16S rDNA, including Variovorax sp, Pseudoxanthomonas mexicana Thierry et al, Acidovorax sp and Methylopila capsulata Doronina et al. These data suggest that this consortium of bacteria performs an incomplete degradation of the simazine

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Monitoring of atrazine treatment on soil bacterial, fungal and atrazine‐degrading communities by quantitative competitive PCR

Cited by 32 publications

References 40 publications

Influence of Glomus etunicatum/Zea mays mycorrhiza on atrazine degradation, soil phosphatase and dehydrogenase activities, and soil microbial community structure

Influence of Glomus etunicatum/Zea mays mycorrhiza on atrazine degradation, soil phosphatase and dehydrogenase activities, and soil microbial community structure

Detection, expression and quantitation of the biodegradative genes in Antarctic microorganisms using PCR

Degradation of simazine by microorganisms isolated from soils of Spanish olive fields

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