Monitoring of Antimicrobial Drug Chloramphenicol Release from Electrospun Nano- and Microfiber Mats Using UV Imaging and Bacterial Bioreporters

Preem, Liis; Bock, Frederik; Hinnu, Mariliis; Putrinš, Marta; Sagor, Kadi; Tenson, Tanel; Meos, Andres; Østergaard, Jesper; Kogermann, Karin

doi:10.3390/pharmaceutics11090487

Cited by 16 publications

(18 citation statements)

References 46 publications

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“…[ 26–28 ] For example, CAM‐loaded polycaprolactone (PCL) nanofibers and, especially, PCL‐ polyethylene oxide (PEO) and microfiber exhibited a very fast release in PBS, reaching in 1 h more than 30% and 90%, respectively. [ 26 ] The difference between such two systems was attributed to the hydrophobic and hydrophilic nature of PCL and PCL‐PEO fibers, respectively. In any case, the strength of the interaction between CAM and such polymers was significantly weaker than with PEDOT.…”

Section: Resultsmentioning

confidence: 99%

Nanotheranostic Interface Based on Antibiotic‐Loaded Conducting Polymer Nanoparticles for Real‐Time Monitoring of Bacterial Growth Inhibition

Enshaei

Puiggalı́-Jou

Valle

et al. 2020

Adv Healthcare Materials

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Conducting polymers have been increasingly used as biologically interfacing electrodes for biomedical applications due to their excellent and fast electrochemical response, reversible doping–dedoping characteristics, high stability, easy processability, and biocompatibility. These advantageous properties can be used for the rapid detection and eradication of infections associated to bacterial growth since these are a tremendous burden for individual patients as well as the global healthcare system. Herein, a smart nanotheranostic electroresponsive platform, which consists of chloramphenicol (CAM)‐loaded in poly(3,4‐ethylendioxythiophene) nanoparticles (PEDOT/CAM NPs) for concurrent release of the antibiotic and real‐time monitoring of bacterial growth is presented. PEDOT/CAM NPs, with an antibiotic loading content of 11.9 ± 1.3% w/w, are proved to inhibit the growth of Escherichia coli and Streptococcus sanguinis due to the antibiotic release by cyclic voltammetry. Furthermore, in situ monitoring of bacterial activity is achieved through the electrochemical detection of β‐nicotinamide adenine dinucleotide, a redox active specie produced by the microbial metabolism that diffuse to the extracellular medium. According to these results, the proposed nanotheranostic platform has great potential for real‐time monitoring of the response of bacteria to the released antibiotic, contributing to the evolution of the personalized medicine.

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Section: Resultsmentioning

confidence: 99%

Nanotheranostic Interface Based on Antibiotic‐Loaded Conducting Polymer Nanoparticles for Real‐Time Monitoring of Bacterial Growth Inhibition

Enshaei

Puiggalı́-Jou

Valle

et al. 2020

Adv Healthcare Materials

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“…Antiviral and antibacterial properties should be studied by using biorelevant pathogens and testing the killing efficiency under external light. The Kogermann research group has experiences in setting up new in vitro/ex vivo/in vivo test systems for the analysis of antipathogen electrospun nanomaterials [107–109] …”

Section: Challenges: From Materials Characterization To In Vitro and In Vivo Efficacy Testsmentioning

confidence: 99%

“…The Kogermann research group has experiences in setting up new in vitro/ex vivo/in vivo test systems forthe analysisofantipathogen electrospun nanomaterials. [107][108][109] These methods can be used andm odificationsc an be performed to improve the models ystem'sb iorelevance. Ex vivo models are one step closer to the in vivo testing, giving already more biorelevant information about the properties of the nanomaterials.…”

Section: Challenges:from Materials Characterizationto In Vitro and In Vivo Efficacy Testsmentioning

confidence: 99%

Personalized Reusable Face Masks with Smart Nano‐Assisted Destruction of Pathogens for COVID‐19: A Visionary Road

Sio

Ding

Focșan

et al. 2021

Chemistry A European J

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The Coronavirus disease 2019 (COVID‐19) emergency has demonstrated that the utilization of face masks plays a critical role in limiting the outbreak. Healthcare professionals utilize masks all day long without replacing them very frequently, thus representing a source of cross‐infection for patients and themselves. Nanotechnology is a powerful tool with the capability to produce nanomaterials with unique physicochemical and antipathogen properties. Here, how to realize non‐disposable and highly comfortable respirators with light‐triggered self‐disinfection ability by bridging bioactive nanofiber properties and stimuli‐responsive nanomaterials is outlined. The visionary road highlighted in this Concept is based on the possibility of developing a new generation of masks based on multifunctional membranes where the presence of nanoclusters and plasmonic nanoparticles arranged in a hierarchical structure enables the realization of a chemically driven and on‐demand antipathogen activities. Multilayer electrospun membranes have the ability to dissipate humidity present within the mask, enhancing the wearability and usability. The photothermal disinfected membrane is the core of these 3D printed and reusable masks with moisture pump capability. Personalized face masks with smart nano‐assisted destruction of pathogens will bring enormous advantages to the entire global community, especially for front‐line personnel, and will open up great opportunities for innovative medical applications.

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“…For these reasons, recently electrospinning technique was used in the treatment and prevention of biofilm-related infections, where the possibility to finely trigger the drug release may have a different impact on the bacterial/biofilm growth [ 39 , 40 , 41 ]. Biofilm is a complex multidimensional, self-sustained community of bacteria producing a matrix consisting of proteins, extracellular DNA, and polysaccharides, which are frequently associated with antibiotic resistance.…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial and Antibiofilm Activity of Curcumin-Loaded Electrospun Nanofibers for the Prevention of the Biofilm-Associated Infections

Salle¹,

Viscusi

Cristo³

et al. 2021

Molecules

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Curcumin extracted from the rhizome of Curcuma Longa has been used in therapeutic preparations for centuries in different parts of the world. However, its bioactivity is limited by chemical instability, water insolubility, low bioavailability, and extensive metabolism. In this study, the coaxial electrospinning technique was used to produce both poly (ε-caprolactone) (PCL)–curcumin and core–shell nanofibers composed of PCL and curcumin in the core and poly (lactic acid) (PLA) in the shell. Morphology and physical properties, as well as the release of curcumin were studied and compared with neat PCL, showing the formation of randomly oriented, defect-free cylindrical fibers with a narrow distribution of the dimensions. The antibacterial and antibiofilm potential, including the capacity to interfere with the quorum-sensing mechanism, was evaluated on Pseudomonas aeruginosa PAO1, and Streptococcus mutans, two opportunistic pathogenic bacteria frequently associated with infections. The reported results demonstrated the ability of the Curcumin-loading membranes to inhibit both PAO1 and S. mutans biofilm growth and activity, thus representing a promising solution for the prevention of biofilm-associated infections. Moreover, the high biocompatibility and the ability to control the oxidative stress of damaged tissue, make the synthesized membranes useful as scaffolds in tissue engineering regeneration, helping to accelerate the healing process.

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Monitoring of Antimicrobial Drug Chloramphenicol Release from Electrospun Nano- and Microfiber Mats Using UV Imaging and Bacterial Bioreporters

Cited by 16 publications

References 46 publications

Nanotheranostic Interface Based on Antibiotic‐Loaded Conducting Polymer Nanoparticles for Real‐Time Monitoring of Bacterial Growth Inhibition

Nanotheranostic Interface Based on Antibiotic‐Loaded Conducting Polymer Nanoparticles for Real‐Time Monitoring of Bacterial Growth Inhibition

Personalized Reusable Face Masks with Smart Nano‐Assisted Destruction of Pathogens for COVID‐19: A Visionary Road

Antimicrobial and Antibiofilm Activity of Curcumin-Loaded Electrospun Nanofibers for the Prevention of the Biofilm-Associated Infections

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