Monitoring of algicidal bacterium, Alteromonas sp. Strain A14 in its application to natural Cochlodinium polykrikoides blooming seawater using fluorescence in situ hybridization

Lee, Bo-Kyung; Katano, Toshiya; Kitamura, Shin-Ichi; Oh, Myung‐Joo; Han, Moonsik

doi:10.1007/s12275-007-0238-9

Cited by 32 publications

(17 citation statements)

References 37 publications

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“…This is an important interaction between protists and bacteria, similar to Mayali and Azam (2004) who reported that heterotrophic protists prevented the lysis of harmful algal species by grazing on algicidal bacteria. In addition, Lee et al (2008) demonstrated that heterotrophic nanoflagellates inhibited the activity of algicidal bacteria against a harmful alga. In this regard, the time lag of the changes in heterotrophic protists could help to control the total bacterial density.…”

Section: Discussionmentioning

confidence: 99%

Algicidal activity of the bacterium, Pseudomonas fluorescens SK09, to mitigate Stephanodiscus hantzschii (Bacillariophyceae) blooms using field mesocosms

Noh

Jung

Kim

et al. 2017

Journal of Freshwater Ecology

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The main goal of this study was to characterize mortality and trophic interactions of Stephanodiscus hantzschii (small centric diatoms) in the presence of an algicidal bacterium, Pseudomonas fluorescens HYK0210-SK09 (SK09) in outdoor mesocosm experiments with an extremely low temperature and also in direct application in a river. SK09 at an inoculation density of 5 £ 10 6 cells/mL (Experiment 1) and SK09 cells immobilized in activated carbon polyvinyl alcohol sponge carriers (Experiment 2) did not appear to attack S. hantzschii in a species-specific manner at temperatures close to freezing. Furthermore, SK09 did not control the diatom blooms when SK09 cells immobilized in the carriers were directly applied in the river. The dynamics of both the heterotrophic flagellate and ciliate abundances showed similar trends to the activity of algicidal bacteria; moreover the heterotrophic protists could not control the total bacterial density. Inorganic nutrient concentrations and conductivity remained unchanged compared with those in the control group. The results obtained in this study and previous results are important for assessing biological control using algicidal bacteria in the wild.

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Section: Discussionmentioning

confidence: 99%

Algicidal activity of the bacterium, Pseudomonas fluorescens SK09, to mitigate Stephanodiscus hantzschii (Bacillariophyceae) blooms using field mesocosms

Noh

Jung

Kim

et al. 2017

Journal of Freshwater Ecology

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“…2), which have been shown to display algicidal activity (Su et al 2010). Alteromonas and Pseudoalteromonas are indeed the most dominant genera amongst bacteria with confirmed algicidal activity (Skerratt et al 2002;Lee et al 2008). Algicidal properties are useful traits in protecting shellfish farms from toxic dinoflagellate blooms (Skerratt et al 2002).…”

Section: Resultsmentioning

confidence: 99%

Identification of epiphytic bacterial communities associated with the brown alga Splachnidium rugosum

et al. 2015

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Marine macroalgae host diverse bacterial communities with which they share a complex array of chemical interactions based on the exchange of nutrients, minerals and secondary metabolites. The brown alga Splachnidium rugosum is a rich source of a valuable fucose-containing sulphated polysaccharide (fucoidan). It grows exclusively in the Southern Hemisphere along temperate shores. While growth and development are dependent on specific microbial interactions, the microbiome of S. rugosum has not been characterized. This study reports on the composition and uniqueness of epiphytic bacterial communities associated with S. rugosum. Sporophytes were collected during winter (July 2012) from the Western Cape (−34°18′ 5.0004″, +18°48′ 59.0004″), South Africa. Culture-based methods relied on a range of selective marine media including marine agar, nutrient sea water agar, nutrient agar and thiosulfatecitrate-bile-salts-sucrose agar. Epiphytic isolates were identified to species level by 16S rRNA gene sequence analysis and encompassed 39 Gram-negative and 2 Gram-positive bacteria. Isolates were classified as Gamma-Proteobacteria, AlphaProteobacteria, Firmicutes or Bacteriodetes. GammaProteobacteria were the most abundant, dominated by Vibrio and Pseudoalteromonas species. Three isolates displayed low sequence identity ( 97 %) with their closest relatives and were grouped into the genera Shewanella, Sphingomonas and Sulfitobacter. All bacterial isolates (41) were screened for antimicrobial activity against indicator strains of Bacillus cereus, Staphylococcus epidermidis, Mycobacterium smegmatis, Micrococcus luteus and Pseudomonas putida. Fifteen isolates (36 %) displayed antimicrobial activity against one or more of the indicator strains. One isolate (Pseudomonas sp.) was active against all strains tested. Splachnidium rugosum is a valuable source for the discovery of bioactive compounds of bacterial origin active against human pathogens.

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“…Samples (each 1 ml) were prepared for catalyzed reporter deposition (CARD)-FISH using the procedure described by Lee et al (2008). Samples fixed for 1 h in 2% formaldehyde were filtered on to 0.2 lm polycarbonate filters (Isopore membrane GTTP filter; Millipore, Eire) and covered with 0.2% agarose (Sigma, USA) to stabilize cell morphology and avoid detachment.…”

Section: Immobilizationmentioning

confidence: 99%

Use of immobilized algicidal bacteria to control natural freshwater diatom blooms

et al. 2011

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The purpose of this investigation was to assess the possible use of algicidal bacteria in conjunction with an immobilization technique for efficient termination of natural blooms of Stephanodiscus concomitant with minimization of adverse effects caused by a single application of bacteria. The performance of Pseudomonas fluorescens cells immobilized on cellulose sponges (CIS) was compared with that of freely suspended cells (FSC) of the organism at low water temperature (WT) of\10°C in co-cultures and natural microcosms. In the co-cultures, CIS resulted in more effective lysis of Stephanodiscus, irrespective of similar bacterivore (Spumella sp.) density, and significantly reduced the concentrations of nitrate and soluble reactive phosphorus (SRP), but not that of silicate, compared with FSC. In the microcosms containing natural freshwater, CIS reduced the densities of Stephanodiscus spp. and Aulacoseira spp. but had no effect on other phytoplankton. Compared with FSC, removal of nutrients by the CIS prevented secondary blooms caused by other phytoplankton. Our results indicate that the CIS affords effective protection of P. fluorescens from low WT and heterotrophs, and restrained regeneration of both SRP and nitrate. Thus, it was demonstrated that the CIS may be an attractive alternative to FSC for control of natural blooms.

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Monitoring of algicidal bacterium, Alteromonas sp. Strain A14 in its application to natural Cochlodinium polykrikoides blooming seawater using fluorescence in situ hybridization

Cited by 32 publications

References 37 publications

Algicidal activity of the bacterium, Pseudomonas fluorescens SK09, to mitigate Stephanodiscus hantzschii (Bacillariophyceae) blooms using field mesocosms

Algicidal activity of the bacterium, Pseudomonas fluorescens SK09, to mitigate Stephanodiscus hantzschii (Bacillariophyceae) blooms using field mesocosms

Identification of epiphytic bacterial communities associated with the brown alga Splachnidium rugosum

Use of immobilized algicidal bacteria to control natural freshwater diatom blooms

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