Monitoring nanoparticle induced cell death in H441 cells using field-effect transistors

Koppenhöfer, D.; Susloparova, Anna; Docter, Dominic; Stauber, Roland H.; Ingebrandt, Sven

doi:10.1016/j.bios.2012.06.031

Cited by 18 publications

(12 citation statements)

References 28 publications

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“…6). Similar to the lung surfactant [32], also epithelial cell of the GI tract are covered by an additional biobarrier, i.e., by mucous matrices [33]. To investigate the impact of mucus associated to GI tract cells on the observed effects, we included the mucus-secreting colorectal cell line HT-29 in our study.…”

Section: Resultsmentioning

confidence: 99%

The protein corona protects against size- and dose-dependent toxicity of amorphous silica nanoparticles

Docter¹,

Bantz²,

Westmeier³

et al. 2014

Beilstein J. Nanotechnol.

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SummaryBesides the lung and skin, the gastrointestinal (GI) tract is one of the main targets for accidental exposure or biomedical applications of nanoparticles (NP). Biological responses to NP, including nanotoxicology, are caused by the interaction of the NP with cellular membranes and/or cellular entry. Here, the physico-chemical characteristics of NP are widely discussed as critical determinants, albeit the exact mechanisms remain to be resolved. Moreover, proteins associate with NP in physiological fluids, forming the protein corona potentially transforming the biological identity of the particle and thus, adding an additional level of complexity for the bio–nano responses.Here, we employed amorphous silica nanoparticles (ASP) and epithelial GI tract Caco-2 cells as a model to study the biological impact of particle size as well as of the protein corona. Caco-2 or mucus-producing HT-29 cells were exposed to thoroughly characterized, negatively charged ASP of different size in the absence or presence of proteins. Comprehensive experimental approaches, such as quantifying cellular metabolic activity, microscopic observation of cell morphology, and high-throughput cell analysis revealed a dose- and time-dependent toxicity primarily upon exposure with ASP30 (Ø = 30 nm). Albeit smaller (ASP20, Ø = 20 nm) or larger particles (ASP100; Ø = 100 nm) showed a similar zeta potential, they both displayed only low toxicity. Importantly, the adverse effects triggered by ASP30/ASP30L were significantly ameliorated upon formation of the protein corona, which we found was efficiently established on all ASP studied. As a potential explanation, corona formation reduced ASP30 cellular uptake, which was however not significantly affected by ASP surface charge in our model. Collectively, our study uncovers an impact of ASP size as well as of the protein corona on cellular toxicity, which might be relevant for processes at the nano–bio interface in general.

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Section: Resultsmentioning

confidence: 99%

The protein corona protects against size- and dose-dependent toxicity of amorphous silica nanoparticles

Docter¹,

Bantz²,

Westmeier³

et al. 2014

Beilstein J. Nanotechnol.

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“…Finally, the rGO sensor arrays were used in proof‐of‐concept experiments to study cell–substrate adhesion of HEK293 cells. In our previous studies silicon‐based field‐effect transistor devices were used for impedance sensing from individual cells .…”

Section: Resultsmentioning

confidence: 99%

Reduced graphene oxide‐based sensing platform for electric cell–substrate impedance sensing

Lanche

Pachauri

Koppenhöfer

et al. 2014

Physica Status Solidi (a)

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Graphene, with its unique electrical properties and biocompatibility, has become a material of choice for the development of biosensor platforms. In this study, a microelectrode array based on reduced graphene oxide (rGO) was constructed and used as a platform for electrical monitoring of cell-substrate adhesion. The rGO-based sensor arrays were designed in order to facilitate sensor pads comparable to the size of individual cells. The sensor chips were fabricated in a scalable manner via site-specific immobilization of graphene oxide flakes onto microelectrode pairs followed by reduction to rGO. The sensor chips were mounted on a measurement platform equipped with a fluidic cell. Electrical characteristics were recorded and fieldeffect behavior was confirmed. Sensors reacted to changes of pH value in the solution. Finally, as a proof-of-concept, the graphene oxide-based sensing platform was used for electrical cell-substrate impedance sensing of individual HEK293 cells in culture.Schematic view of the rGO-based sensor chip for electrical cell-substrate adhesion assays.

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“…Another promising non-invasive, label-free analysis system to assess various physical (Sekitani et al, 2009), chemical and biological (Lin and Yan, 2012) parameters are organic field-effect transistors. This technology has the potential to facilitate the fabrication of low-cost, printable and highly sensitive biosensors for integration into lab-on-a-chip and micro-total analysis platforms (Koppenhofer et al, 2013, Someya et al, 2010, Torsi et al, 2002. Another electro-analytical method used for the analysis of physiological barriers (e.g.…”

Section: Sensing Strategies For Microfluidic Cell Analysis Systemsmentioning

confidence: 99%

Recent advances and future applications of microfluidic live-cell microarrays

Rothbauer

Wartmann

Charwat

et al. 2015

Biotechnology Advances

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Monitoring nanoparticle induced cell death in H441 cells using field-effect transistors

Cited by 18 publications

References 28 publications

The protein corona protects against size- and dose-dependent toxicity of amorphous silica nanoparticles

The protein corona protects against size- and dose-dependent toxicity of amorphous silica nanoparticles

Reduced graphene oxide‐based sensing platform for electric cell–substrate impedance sensing

Recent advances and future applications of microfluidic live-cell microarrays

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