Monitoring Metabolic Changes in Response to Chemotherapies in Cancer with Raman Spectroscopy and Metabolomics

Innovative Approaches for Drug Discovery: Quantifying Drug Distribution and Response with Raman Imaging

“…Additionally, given the complexity of the biological environment, the drug Raman spectrum could heavily overlap with native biomolecules, leading to difficulty in quantifying drug concentration even if sensitivity is not a problem. Instead, by leveraging the chemically rich information contained within Raman spectra, the examination of changes in cellular content such as lipid, protein, and nucleic acid at a subcellular scale in response to drug treatment is an alternative to understanding drug dynamics. , A multitude of studies have been published in recent years on applying RS to identify biomarkers and biochemical changes associated with drug treatments. − For example, Hammoud et al explored the impact of EGFR mutations on cellular responses to different TKIs . By identification of differences in Raman spectra before and after drug treatment, large spectral changes were detected that indicated alterations in cellular composition.…”

Exploring the cellular antioxidant mechanism against cytotoxic silver nanoparticles: a Raman spectroscopic analysis

“…Unlike traditional methods, Raman spectroscopy provides label-free and non-invasive probing capabilities, allowing researchers to directly observe molecular changes within living cells. 30,31 Indeed, Raman microspectroscopy has been employed for the characterization of biochemical evolution during cellular differentiation, 32,33 neuronal cell networking and separation, 34 metabolic changes in cancer cells 35,36 and apoptosis. 37,38 In the nano-toxicology context, this technique can have the advantageous ability to provide detailed information on cellular component alterations, which can be correlated with cytotoxic responses, oxidative stress, or inflammation induced by nanomaterial exposure.…”