Monitoring Intracellular Zn2+ Using Fluorescent Sensors: Facts and Artifacts

Kiedrowski, Lech

doi:10.1007/978-1-4939-6918-0_12

Cited by 1 publication

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“…Free vs bound metal fractions. The fractions of zinc which are free, or available, to react with the sulphide in Timm's method, or with exogenous chelating agents, have been given many names: Free 106-108 , available 109 , histochemically reactive 110 , chelatable 24,111,112 , exchangeable 113 , mobile 114,115 , loosely bound 116,117 and -for iron -labile iron pool (LIP) 118 , labile cell iron (LCI) 119 and non-heme iron 120 . Free metal ions are always coordinated by water molecules or other ligands, as illustrated for zinc 121 .…”

Section: Technical Validationmentioning

confidence: 99%

A Timm-Nissl multiplane microscopic atlas of rat brain zincergic terminal fields and metal-containing glia

et al. 2023

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The ability of Timm’s sulphide silver method to stain zincergic terminal fields has made it a useful neuromorphological marker. Beyond its roles in zinc-signalling and neuromodulation, zinc is involved in the pathophysiology of ischemic stroke, epilepsy, degenerative diseases and neuropsychiatric conditions. In addition to visualising zincergic terminal fields, the method also labels transition metals in neuronal perikarya and glial cells. To provide a benchmark reference for planning and interpretation of experimental investigations of zinc-related phenomena in rat brains, we have established a comprehensive repository of serial microscopic images from a historical collection of coronally, horizontally and sagittally oriented rat brain sections stained with Timm’s method. Adjacent Nissl-stained sections showing cytoarchitecture, and customised atlas overlays from a three-dimensional rat brain reference atlas registered to each section image are included for spatial reference and guiding identification of anatomical boundaries. The Timm-Nissl atlas, available from EBRAINS, enables experimental researchers to navigate normal rat brain material in three planes and investigate the spatial distribution and density of zincergic terminal fields across the entire brain.

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