Monitoring influenza hemagglutinin and glycan interactions using surface plasmon resonance

Suenaga, Emi; Mizuno, Hiroshi; Penmetcha, Kumar

doi:10.1016/j.bios.2011.12.003

Cited by 66 publications

(53 citation statements)

References 38 publications

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“…Influenza occurs all over the world, causing death and imposing severe burdens on human health and the economy. Developing detection systems is crucial and will prevent severe consequences 25 . Therefore, we used the present system for detection of an influenza strain.…”

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confidence: 99%

An angular fluidic channel for prism-free surface-plasmon-assisted fluorescence capturing

et al. 2013

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Surface plasmon excitation provides stronger enhancement of the fluorescence intensity and better sensitivity than other sensing approaches but requires optimal positioning of a prism to ensure optimum output of the incident light. Here we describe a simple, highly sensitive optical sensing system combining surface plasmon excitation and fluorescence to address this limitation. V-shaped fluidic channels are employed to mimic the functions of a prism, sensing plate, and flow channel in a single setup. Superior performance is demonstrated for different biomolecular recognition reactions on a self-assembled monolayer, and the sensitivity reaches 100 fM for biotin-streptavidin interactions. Using an antibody as a probe, we demonstrate the detection of intact influenza viruses at 0.2 HA units ml À 1 levels. The convenient sensing system developed here has the advantages of being prism-free and requiring less sample (1-2 ml), making this platform suitable for use in situations requiring low sample volumes.

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confidence: 99%

An angular fluidic channel for prism-free surface-plasmon-assisted fluorescence capturing

et al. 2013

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“…Specific HA-glycan affinity has been exploited as the basis for isolation of various types of influenza viruses [25]. In our previous study we described a MPs-based isolation method for standard viral proteins labeled with CdS [26].…”

Section: Resultsmentioning

confidence: 99%

“…The other two conditions optimized directly included the labeled HA isolation reaction. Tested conditions included six temperatures (5,25,35,45, 55 and 65 °C) and reaction times (5,10,15,20,30,45 and 60 min). From the results it follows that best signals were obtained at temperatures higher than 35 °C and the temperature of 45 °C was established as the best, based on the Cd peak results (blue columns in Figure 4B) and HA peak (red rhombi in Figure 4B), which correspond to the range of body temperatures of humans and small animals, especially birds, which serve as influenza reservoirs [32].…”

Section: Optimization Of the Automated Isolation Proceduresmentioning

confidence: 99%

“…After that biotinyled multivalent-glycan (01-039a [Neu5Acα2-6Galβ1-4GlcNAcβ1-PAA-biotin], 20 μL) which was used according to Suenaga at al. [25], supplied by GlycoTech (Gaithersburg, MD, USA), were added to each of the wells and incubated (30 min, 25 °C, 400 rpm). In the next step each well was washed three times with PB (100 μL and sample (vaccine HA labeled by CdTe, 20 μL) was added.…”

Section: The Automated Isolation Proceduresmentioning

confidence: 99%

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Beads-Based Electrochemical Assay for the Detection of Influenza Hemagglutinin Labeled with CdTe Quantum Dots

et al. 2013

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Abstract:In this study we describe a beads-based assay for rapid, sensitive and specific isolation and detection of influenza vaccine hemagglutinin (HA). Amplification of the hemagglutinin signal resulted from binding of an electrochemical label as quantum dots (QDs). For detection of the metal and protein part of the resulting HA-CdTe complex, two differential pulse voltammetric methods were used. The procedure includes automated robotic isolation and electrochemical analysis of the isolated product. The isolation procedure was based on the binding of paramagnetic particles (MPs) with glycan (Gly), where glycan was used as the specific receptor for linkage of the QD-labeled hemagglutinin.

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“…These ligands can be used for the development of a rapid bioanalytical sensor. Suenaga et al 231 developed an SPR-based method for analyzing the glycan-HA interactions, using chemically synthesized biotinylated multivalent glycans.…”

Section: Affinity and Aptamer Spr Biosensormentioning

confidence: 99%

Nanoscale virus biosensors: state of the art

Krejčová¹,

Michálek²,

Rodrigo³

et al. 2015

NDD

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Abstract:Since the beginning of the new millennium, viruses have shown huge epidemiological and pandemic potential: severe acute respiratory syndrome (SARS) in 2002, pandemic swine flu in 2009, and last but not least the West African Ebola outbreak in 2014. The occurrence and spread of the new virus in pandemic dimension poses a threat to the health and lives of 7 billion people worldwide. There is a growing urgency for highly sensitive and selective detection techniques, usable for a wide number of applications, including disease diagnosis, pharmaceutical research, agriculture, as well as preventive measures. Nanobiosensors represent a new promising tool for virus detection. This review gives a brief survey of the issue of viral detection, comprising diagnostics of target structure of viruses such as nucleic acids or proteins. This review covers different detection principles, methods of fabrication, and applications of virus biosensors.

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Monitoring influenza hemagglutinin and glycan interactions using surface plasmon resonance

Cited by 66 publications

References 38 publications

An angular fluidic channel for prism-free surface-plasmon-assisted fluorescence capturing

An angular fluidic channel for prism-free surface-plasmon-assisted fluorescence capturing

Beads-Based Electrochemical Assay for the Detection of Influenza Hemagglutinin Labeled with CdTe Quantum Dots

Nanoscale virus biosensors: state of the art

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