Monitoring indole alkaloid production by Penicillium digitatum during infection process in citrus by Mass Spectrometry Imaging and molecular networking

Costa, Jonas Henrique; Bazioli, Jaqueline Moraes; Araújo, Éder de Vilhena; Vendramini, Pedro Henrique; Porto, Mariana Cristina C. de Freitas; Eberlin, Marcos N.; Souza-Neto, Jayme A.; Fill, Taícia Pacheco

doi:10.1016/j.funbio.2019.03.002

Cited by 25 publications

(31 citation statements)

References 28 publications

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“…3). These compounds are part of the tryptoquialanines biosynthetic pathway 32 and were previously detected during DESI-MSI analysis of oranges infected with the green mold disease 23 .…”

Section: Resultsmentioning

confidence: 92%

“…Recently, 1 was demonstrated as an insecticidal compound against Ae. aegypti larvae 23 ; the antifungal activity can provide more understanding about the role of the tryptoquialanines in the citrus-pathogen environment once these compounds are not required for P. digitatum virulence 34 .…”

Section: Resultsmentioning

confidence: 99%

“…For in vivo co-culture, mature oranges ( Citrus sinensis ) were surface sterilized and wounded 23 . A small piece of PDA containing P. citrinum was inoculated in the wound site.…”

Section: Methodsmentioning

confidence: 99%

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Antifungal potential of secondary metabolites involved in the interaction between citrus pathogens

Costa

Wassano

Angolini

et al. 2019

Sci Rep

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Numerous postharvest diseases have been reported that cause substantial losses of citrus fruits worldwide. Penicillium digitatum is responsible for up to 90% of production losses, and represent a problem for worldwide economy. In order to control phytopathogens, chemical fungicides have been extensively used. Yet, the use of some artificial fungicides cause concerns about environmental risks and fungal resistance. Therefore, studies focusing on new approaches, such as the use of natural products, are getting attention. Co-culture strategy can be applied to discover new bioactive compounds and to understand microbial ecology. Mass Spectrometry Imaging (MSI) was used to screen for potential antifungal metabolites involved in the interaction between Penicillium digitatum and Penicillium citrinum. MSI revealed a chemical warfare between the fungi: two tetrapeptides, deoxycitrinadin A, citrinadin A, chrysogenamide A and tryptoquialanines are produced in the fungi confrontation zone. Antimicrobial assays confirmed the antifungal activity of the investigated metabolites. Also, tryptoquialanines inhibited sporulation of P. citrinum. The fungal metabolites reported here were never described as antimicrobials until this date, demonstrating that co-cultures involving phytopathogens that compete for the same host is a positive strategy to discover new antifungal agents. However, the use of these natural products on the environment, as a safer strategy, needs further investigation. This paper aimed to contribute to the protection of agriculture, considering health and ecological risks.

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“…3). These compounds are part of the tryptoquialanines biosynthetic pathway 32 and were previously detected during DESI-MSI analysis of oranges infected with the green mold disease 23 .…”

Section: Resultsmentioning

confidence: 92%

Section: Resultsmentioning

confidence: 99%

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Antifungal potential of secondary metabolites involved in the interaction between citrus pathogens

Costa

Wassano

Angolini

et al. 2019

Sci Rep

Self Cite

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“…Deleting a tryptoquialanine-production-related gene tqaA in Pd resulted in complete loss of tryptoquialanine production but no significant difference was observed in Pd virulence between the ∆tqaA mutant and the wild type strain, suggesting that tryptoquialanines are not involved in Pd pathogenicity [44]. Noticeably, tryptoquialanines may be involved in the protection of decayed citrus from insects because tryptoquialanine A shows high insecticide activity for Aedes Aegypti [39].…”

Section: Mycotoxin During Pd Infectionmentioning

confidence: 99%

“…Mycotoxin is a severe problem for public health and the analysis of potential mycotoxins in fruits infected by postharvest fungal pathogens are important for their quality control and safety [38]. Thermogenic alkaloids, including tryptoquialanine A and tryptoquialanine C, were detected in citrus fruit infected by Pd [38][39][40]. However, no other mycotoxins have been reported in Pd infection on citrus fruit.…”

Section: Mycotoxin During Pd Infectionmentioning

confidence: 99%

Citrus Postharvest Green Mold: Recent Advances in Fungal Pathogenicity and Fruit Resistance

et al. 2020

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As the major postharvest disease of citrus fruit, postharvest green mold is caused by the necrotrophic fungus Penicillium digitatum (Pd), which leads to huge economic losses worldwide. Fungicides are still the main method currently used to control postharvest green mold in citrus fruit storage. Investigating molecular mechanisms of plant–pathogen interactions, including pathogenicity and plant resistance, is crucial for developing novel and safer strategies for effectively controlling plant diseases. Despite fruit–pathogen interactions remaining relatively unexplored compared with well-studied leaf–pathogen interactions, progress has occurred in the citrus fruit–Pd interaction in recent years, mainly due to their genome sequencing and establishment or optimization of their genetic transformation systems. Recent advances in Pd pathogenicity on citrus fruit and fruit resistance against Pd infection are summarized in this review.

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Alkaloids of forest fungi: diversity and therapeutic applications

Anaele,

Nwafor

2025

Forest Fungi

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Monitoring indole alkaloid production by Penicillium digitatum during infection process in citrus by Mass Spectrometry Imaging and molecular networking

Cited by 25 publications

References 28 publications

Antifungal potential of secondary metabolites involved in the interaction between citrus pathogens

Antifungal potential of secondary metabolites involved in the interaction between citrus pathogens

Citrus Postharvest Green Mold: Recent Advances in Fungal Pathogenicity and Fruit Resistance

Alkaloids of forest fungi: diversity and therapeutic applications

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