Monitoring in vivo Skin Hydration by Liposomes Using Infrared Spectroscopy in Conjunction with Tape Stripping

Boddé, Harry E.; Pechtold, Louk A. R. M.; Subnel, M. T. A.; Haan, F. H. N. de

doi:10.1007/978-3-642-48391-2_14

Cited by 8 publications

(8 citation statements)

References 8 publications

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“…The curve profile very much resembles the ones observed by Bodde et al [22] for an untreated control site. A higher value of skin hydration is known to induce an increase in vibrational frequency of CH 2 stretchings [29].…”

Section: Tewl Skin Capacitance and Atr-ftir As A Function Of Sc Depthsupporting

confidence: 73%

“…A shift toward a higher wavenumber of these vibrational bands indicates a change in the conformer populations, increasing numbers of gauche conformer [28]. These changes in lipidic order could be related to their fluidity and, consequently, to the transdermal drug delivery in the presence of enhancer penetrants like liposomes [22], owing to the role that SC lipids play in skin barrier function. Frequencies of CH 2 asymmetric and symmetric stretching of measured human SC (table 3) are plotted as a function of strip number for treated sites and untreated skin control in figures 5 and 6, respectively.…”

Section: Tewl Skin Capacitance and Atr-ftir As A Function Of Sc Depthmentioning

confidence: 99%

“…The depth of penetration is proportional to the wavelength of the infared radiation and inversely proportional to the wavenumber. At the wavenumber interval under study, the depth of penetration in the skin is about 1 Ìm [22].…”

Section: Monitoring Tewl Skin Capacitance and Atr-ftir As A Functionmentioning

confidence: 99%

“…This non-invasive technique of infrared spectroscopy has been used to evaluate 'in vivo' the structural SC lipid order, measuring vibrational frequencies of methylene groups of stratum corneum alkyl chains [22].…”

Section: Introductionmentioning

confidence: 99%

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The Effect of Liposomes on Skin Barrier Structure

Coderch

Pera

Pérez-Cullell

et al. 1999

Skin Pharmacol Physiol

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The present work deals with the ‘in vivo’ stripping technique to evaluate the percutaneous absorption of sodium fluorescein (NaFl) vehiculized in two different liposome preparations formed by phosphatidylcholine (PC) and lipids mimicking the stratum corneum (SC; ceramides, cholesterol, palmitic acid and cholesteryl sulphate), respectively. Furthermore, the possible effect of these vesicles on the SC lipid alkyl chain conformational order were evaluated at different depths of SC by non-invasive biophysical techniques: Corneometer, Tewameter and especially ATR-FTIR. The results of NaFl percutaneous absorption indicate the highest penetration in the case of incorporation in PC liposomes, which could be related to the increase in SC lipid disorder detected by ATR-FTIR, i.e. a decrease in skin barrier function. On the other hand, SC lipid liposomes have been shown to have a higher affinity for SC owing to the high amount of NaFl found in this layer, suggesting a greater reservoir capacity of SC when similar lipid composition formulation is applied. A lipid order increase is observed by infrared spectroscopy, when these types of liposomes are topically applied, resulting in a strong barrier effect. These results could be useful in designing specific liposomal topical applications.

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Section: Tewl Skin Capacitance and Atr-ftir As A Function Of Sc Depthsupporting

confidence: 73%

Section: Tewl Skin Capacitance and Atr-ftir As A Function Of Sc Depthmentioning

confidence: 99%

Section: Monitoring Tewl Skin Capacitance and Atr-ftir As A Functionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The Effect of Liposomes on Skin Barrier Structure

Coderch

Pera

Pérez-Cullell

et al. 1999

Skin Pharmacol Physiol

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“…Boddé et al 9 and Wichrowski et al 10 studied hydration of the skin applying cosmetic moisturizers using ATR-FTIR.…”

Section: Introductionmentioning

confidence: 99%

Band Analysis of Hydrated Human Skin Stratum Corneum Attenuated Total Reflectance Fourier Transform Infrared Spectra In Vivo

Lucassen

1998

J. Biomed. Opt.

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Water content is an important factor for skin condition. The determination of the hydration state of the skin is necessary to obtain basic knowledge about the penetration and loss of water in the skin stratum corneum. Attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy is used to measure hydration of the stratum corneum. In this study we apply direct band fitting of the water bending, combination, and OH stretch bands over the 4000-650 cm-1 wave number range. Measurements are performed on the volar aspect of the forearm using a Nicolet 800 FTIR spectrometer equipped with an ATR unit with a ZnSe crystal. Hydration of the skin is obtained by occlusion keeping the forearm pressed onto the crystal. Spectra are recorded before and after occlusion up to 30 min. The spectra are fitted with a nonlinear least-squares algorithm with Gaussian bands. Separate band fits of water, normal stratum corneum, and occluded hydrated stratum corneum spectra are obtained yielding band parameters of the individual water contributions in the bending mode at 1640 cm-1, the combination band at 2125 cm-1, and the OH stretches in the hydrated skin stratum corneum spectra. A scaling factor representing the contribution of the water spectrum into the skin stratum corneum spectrum is determined during the occlusion process. In comparison to the dependence of the infrared absorbance ratio in time used by Potts, our scaling factor shows a more distinct transition from enhanced signal due to increased contact area to extra signal due to water content at maximum contact area. Band fit analysis of hydrated skin stratum corneum ATR-FTIR spectra offers the possibility for quantitative determination of individual water band parameters. © 1998 Society of Photo-Optical Instrumentation Engineers.

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Crystal Effect on Penetration Depth in Attenuated Total Reflectance Fourier-transform Infrared Study of Human Skin

Snieder¹,

Hansen²

1997

Progress in Fourier Transform Spectroscopy

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Monitoring in vivo Skin Hydration by Liposomes Using Infrared Spectroscopy in Conjunction with Tape Stripping

Cited by 8 publications

References 8 publications

The Effect of Liposomes on Skin Barrier Structure

The Effect of Liposomes on Skin Barrier Structure

Band Analysis of Hydrated Human Skin Stratum Corneum Attenuated Total Reflectance Fourier Transform Infrared Spectra In Vivo

Crystal Effect on Penetration Depth in Attenuated Total Reflectance Fourier-transform Infrared Study of Human Skin

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