Monitoring In Vitro Extracellular Matrix Protein Conformations in the Presence of Biomimetic Bone-Regeneration Scaffolds Using Functionalized Gold-Edge-Coated Triangular Silver Nanoparticles

Barroso, Laura G. Rodriguez; Azaman, Farah Alwani; Pogue, Robert; Devine, Declan M.; Fournet, Margaret Brennan

doi:10.3390/nano13010057

Cited by 4 publications

(6 citation statements)

References 28 publications

(36 reference statements)

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“…Fn has an uncoiled conformation when in acidic or high salt conditions, whereas within low salt conditions it shows a "coiled compact" conformation [61]. In previous studies where Fn conformational changes are analyzed within different pH environments, Fn is reported to have a hydrodynamic radius of 23 nm when compacted, and when it extends the radius increases up to 130 nm for single Fn strands [43,44]. Fn can form much larger strands as networks and fibrils can develop and be mediated within the extracellular matrix (ECM) [62].…”

Section: Discussionmentioning

confidence: 99%

“…Growth percentage was calculated using the following equation (Equation ( 1 Nanoplate protection against etching by chloride ions present in physiological environments was achieved by covering the edges of the nanoplates with gold to keep their plasmonic resonance properties. Gold edge coating of the TSNP was carried out following the method reported by [44], consisting of the use of 20 µL of 0.5 mM HAuCl 4 salt and 18.9 µL of 10 mM ascorbic acid per 1 mL of TSNP solution. In this method, there is a reduction in AuCl 4 and inhibition of Ag oxidation at the surface of the TSNP, resulting in the deposition of a thin layer of gold in the edges of the TSNP due to galvanic replacement.…”

Section: Evaluation Of Antimicrobial Activitymentioning

confidence: 99%

“…Brennan-Fournet et al (2015) successfully detected conformational changes in fibronectin, a protein related to tumor growth and cell survival, using AuTSNPs [43]. Rodriguez Barroso et al (2023) monitored fibronectin behavior overtime within different complex cellular environments [44]. Vinayagam et al (2018) built a nanoprobe by conjugating single-strand DNA to the TSNP surface, for specific detection of dengue virus [45].…”

Section: Introductionmentioning

confidence: 99%

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Triangular Silver Nanoparticles Synthesis: Investigating Potential Application in Materials and Biosensing

Barroso¹,

Garcia²,

Mojićević³

et al. 2023

Applied Sciences

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Triangular silver nanoplates (TSNPs) exhibit unique optical and antimicrobial properties due to their shape, sharp edges, and vertices. In this study, TSNPs were incorporated into biopolymer blends (bacterial cellulose (BC) with polylactic acid (PLA), polycaprolactone (PCL), and polyhydroxybutyrate (PHB)). Antimicrobial activity of materials was tested against Escherichia coli ATCC 95922 and Staphylococcus aureus ATCC 25923 (106 CFU/mL). After incubation (24 h at 37 °C, 100 rpm), optical density was measured at 630 nm. In order to assess biosensing applications, specifically fibronectin (Fn) behavior, TSNPs were protected with gold (AuTSNP) and analyzed via sucrose sensitivity test and monitored by localized surface plasmon resonance (LSPR). Additionally, AuTSNPs were coated with polyethylene glycol (PEGAuTSNP). Fibronectin functionalization of PEGAuTSNPs and pH-conformation was monitored (FnPEGAuTSNP). Eventually, adequate Fn and anti-Fn antibody concentrations were determined. BC/PHB/TSNPs showed antimicrobial activity against E. coli and S. aureus with 80 and 95% of growth inhibition, respectively. The sucrose sensitivity test indicated that the LSPRλmax of the spectra is directly proportional to the sucrose concentration. LSPRλmax of Fn-PEGAuTSNPs at pH 7 and pH 4 were measured at 633 and 643 nm, respectively. A total of 5 µg of Fn was determined to be adequate concentration, while 0.212 mg/mL of anti-Fn antibody indicatied system saturation.

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Section: Discussionmentioning

confidence: 99%

Section: Evaluation Of Antimicrobial Activitymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Triangular Silver Nanoparticles Synthesis: Investigating Potential Application in Materials and Biosensing

Barroso¹,

Garcia²,

Mojićević³

et al. 2023

Applied Sciences

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“…Their unique properties and broad-spectrum antimicrobial activity make them valuable in healthcare applications, including the self-hygiene of sports prostheses for children. By incorporating AgNPs into prosthetic materials, a hygienic environment can be promoted, reducing the risk of infections and enhancing the overall well-being (Akhavan et al, 2018;Sarraf et al, 2018;Vale et al, 2019;Xie et al, 2019;Devaraj et al, 2020;Sohi et al, 2021;Bee et al, 2022;Nathanael et al, 2022;Padmanabhan et al, 2022;Rodriguez Barroso et al, 2023).…”

Section: Journal Of Disability Research 2023mentioning

confidence: 99%

A Review of Children’s Prosthetic Self-hygiene Using Antibacterial Silver Nanoparticles (AgNPs)

Alnaser,

Siddiqui,

Aijaz

et al. 2023

Journal of Disability Research

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Sports prostheses represent a critical facet in enhancing the mobility and quality of life for children with limb loss. However, prolonged usage of these prosthetic devices introduces substantial challenges related to hygiene and the heightened risk of infection. This comprehensive review endeavors to delve into the untapped potential of utilizing antibacterial silver nanoparticles (AgNPs) to foster self-hygiene practices in pediatric sports prostheses. AgNPs have emerged as a compelling area of research, largely attributable to their formidable antibacterial properties, offering revolutionary prospects in the realm of antibacterial therapy. Central to this exploration is an in-depth analysis of their antibacterial activity, elucidating their intricate mechanisms of action against bacterial agents. The unique physicochemical attributes, characterized by their diminutive size and expansive surface area, synergize to elevate their antibacterial efficacy significantly. The antibacterial mechanisms involve the controlled release of silver ions, which disrupt bacterial cell membranes, impede vital cellular processes, and provoke oxidative stress within the pathogens. Moreover, they exhibit the propensity for synergistic interactions when employed in tandem with conventional antibiotics, thereby positioning themselves as promising candidates for combination therapy. This review further encompasses a comprehensive survey of diverse methodologies employed for the synthesis of AgNPs and their multifarious application in various domains, including the realm of prosthetics and medicine. Nevertheless, as with any emerging technology, the implementation of AgNPs does not come without its challenges. Issues pertinent to the stability of AgNPs, potential toxicity concerns, and the development of bacterial resistance necessitate critical consideration for their successful integration into pediatric sports prostheses. In summation, AgNPs epitomize a compelling arsenal of antibacterial agents, propelling the possibilities of innovative therapeutic strategies in the battle against bacterial infections. This review underscores their remarkable potential while underscoring the imperative need for further research and development to unlock their full efficacy in the context of pediatric sports prostheses and beyond.

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“…The currently available techniques for protein characterization that are able to detect conformations and structural changes in proteins require expensive instrumentation and extensive data analysis, and their signals are obstructed by cellular noise when applied in complex biological environments [9]. The necessity for the development of enzyme-free analytical assays has arisen in order to overcome ELISA assays' limitations [10].…”

Section: Introductionmentioning

confidence: 99%

Triangular Silver Nanoplates as a Bioanalytical Tool: Potential COVID-19 Detection

Barroso¹,

Garcia²,

Mojićević³

et al. 2023

IJMS

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Nanotechnology offers new possibilities in molecular diagnostics, with nanoparticles gaining attention as biosensor upgrades. This study evaluates gold-coated silver nanoplates coated with PEG for enhanced protection, aiming to detect Spike protein with higher sensitivity, and emphasizes the importance of considering complex environments and appropriate controls for specific binding and accurate analysis. The sensitivity of antibody-coated PEGAuTSNPs as tools for immunoassays is demonstrated through fibronectin (Fn)– anti-fibronectin binding within an isolated extracellular matrix as a complex and native environment of Fn. Moreover, the optimal functionalization volume of Spike protein was determined (4 µg/mL of PEGAuTSNP). Anti-Spike was added to confirm binding, while the TJP1 protein was used as a negative control. The same experiment was used in the presence of horse serum to simulate a complex environment. According to Localized Surface Plasmon Resonance analysis and Dynamic Light Scattering size measurements, anti-Spike exhibited a stronger affinity for the nanoplates, causing TJP1 to be replaced by the antibody on the nanoplates’ surface. Future research will involve exploring alternative complex environments, filtering larger molecules, and the optimization of immunoassay performance.

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Monitoring In Vitro Extracellular Matrix Protein Conformations in the Presence of Biomimetic Bone-Regeneration Scaffolds Using Functionalized Gold-Edge-Coated Triangular Silver Nanoparticles

Cited by 4 publications

References 28 publications

Triangular Silver Nanoparticles Synthesis: Investigating Potential Application in Materials and Biosensing

Triangular Silver Nanoparticles Synthesis: Investigating Potential Application in Materials and Biosensing

A Review of Children’s Prosthetic Self-hygiene Using Antibacterial Silver Nanoparticles (AgNPs)

Triangular Silver Nanoplates as a Bioanalytical Tool: Potential COVID-19 Detection

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