Monitoring in Real Time with a Microelectrode the Release of Reactive Oxygen and Nitrogen Species by a Single Macrophage Stimulated by its Membrane Mechanical Depolarization

Amatore, Christian; Arbault, Stéphane; Bouton, Cécile; Coffi, Karen; Drapier, Jean‐Claude; Ghandour, Hala; Tong, Yuehong

doi:10.1002/cbic.200500359

Cited by 152 publications

(194 citation statements)

References 59 publications

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“…Going a step further, we have also conducted in vitro studies to test the performance of the graphene-hemin NO sensors for realtime detection of extracellular NO generated from RAW 264.7 macrophage cells. It is well known that macrophage cells can generate NO with expression of inducible NO synthase (iNOS) when stimulated by lipopolysaccharide (LPS) [46][47][48][49] . RAW 264.7 cells were seeded at 5 Â 10 5 cells per ml (cell suspension) onto the graphene-hemin device on the SiO 2 substrate (inset, Fig.…”

Section: Fabrication and Characterization Of Graphene-hemin Sensorsmentioning

confidence: 99%

Real-time electrical detection of nitric oxide in biological systems with sub-nanomolar sensitivity

et al. 2013

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Real-time monitoring of nitric oxide concentrations is of central importance for probing the diverse roles of nitric oxide in neurotransmission, cardiovascular systems and immune responses. Here we report a new design of nitric oxide sensors based on hemin-functionalized graphene field-effect transistors. With its single atom thickness and the highest carrier mobility among all materials, graphene holds the promise for unprecedented sensitivity for molecular sensing. The non-covalent functionalization through p-p stacking interaction allows reliable immobilization of hemin molecules on graphene without damaging the graphene lattice to ensure the highly sensitive and specific detection of nitric oxide. Our studies demonstrate that the graphene-hemin sensors can respond rapidly to nitric oxide in physiological environments with a sub-nanomolar sensitivity. Furthermore, in vitro studies show that the graphene-hemin sensors can be used for the detection of nitric oxide released from macrophage cells and endothelial cells, demonstrating their practical functionality in complex biological systems.

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Section: Fabrication and Characterization Of Graphene-hemin Sensorsmentioning

confidence: 99%

Real-time electrical detection of nitric oxide in biological systems with sub-nanomolar sensitivity

et al. 2013

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“…Some of these utilize direct detection of diverse reactive species based on their differential redox potentials, while others use electrodes modified with molecules or proteins with electrocatalytic activity towards the species of interest. Single-cell amperometric measurements are another elegant approach for parallel detection of ROS and RNS production [55][56][57].…”

Section: Electrochemistrymentioning

confidence: 99%

Redox regulation of calcium ion channels: Chemical and physiological aspects

et al. 2011

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a b s t r a c tReactive oxygen species (ROS) are increasingly recognized as second messengers in many cellular processes. While high concentrations of oxidants damage proteins, lipids and DNA, ultimately resulting in cell death, selective and reversible oxidation of key residues in proteins is a physiological mechanism that can transiently alter their activity and function. Defects in ROS producing enzymes cause disturbed immune response and disease.Changes in the intracellular free Ca 2+ concentration are key triggers for diverse cellular functions. Ca 2+ homeostasis thus needs to be precisely tuned by channels, pumps, transporters and cellular buffering systems. Alterations of these key regulatory proteins by reversible or irreversible oxidation alter the physiological outcome following cell stimulation. It is therefore necessary to understand which proteins are regulated and if this regulation is relevant in a physiological-and/or pathophysiological context. Because ROS are inherently difficult to identify and to measure, we first review basic oxygen redox chemistry and methods of ROS detection with special emphasis on electron paramagnetic resonance (EPR) spectroscopy. We then focus on the present knowledge of redox regulation of Ca 2+ permeable ion channels such as voltage-gated (CaV) Ca 2+ channels, transient receptor potential (TRP) channels and Orai channels.© 2011 Elsevier Ltd. All rights reserved. Basic redox chemistry of oxygenMany chemical processes are linked to the exchange of electrons between two or more molecular entities. The transfer of one (or more) electron(s) is associated with oxidation (loss of electron) and reduction (gain of electron) of the components. Such reactions are also known as redox reactions. The processes of reactive oxygen species (ROS) formation and elimination are exclusively of redox nature.Molecular oxygen is the precursor of all ROS. It contains two unpaired electrons in separate (antibonding) orbitals in its outer electron sphere and is thus, by definition, a radical. Radicals have at least one unpaired electron in their orbital system and usually show high reactivity; transition metal ions also may have unpaired electrons, but are not called radicals. Although having radical character, molecular oxygen is chemically rather inert (luckily!), because high * Corresponding authors at: Institut für Biophysik, Gebäude 58, Universität des Saarlandes, D-66421 Homburg/Saar, Germany. Tel.: +49 6841 1626453; fax: +49 6841 1626060.E-mail addresses: ivan.bogeski@uks.eu (I. Bogeski), barbara.niemeyer@uks.eu (B.A. Niemeyer).

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“…In neuroscience research, microelectrode have been used extensively for the determination of oxygen consumption, reactive oxygen and nitrogen species, [11][12][13] and for the analytical detection of electrochemically active neurotransmitters both in vivo and in vitro. 12,[14][15][16] Mirkin's group pioneered the application of electrochemical probes for the measurement of redox properties of living cells 17 .…”

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confidence: 99%

Electrochemical Nanoprobes for Single-Cell Analysis

et al. 2014

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The measurement of key molecules in individual cells with minimal disruption to the biological milieu is the next frontier in single-cell analyses. Nanoscale devices are ideal analytical tools because of their small size and their potential for high spatial and temporal resolution recordings. Here, we report the fabrication of disk-shaped carbon nanoelectrodes whose radius can be precisely tuned within the range 5-200 nm. The functionalization of the nanoelectrode with platinum allowed the monitoring of oxygen consumption outside and inside a brain slice. Furthermore, we show that nanoelectrodes of this type can be used to impale individual cells to perform electrochemical measurements within the cell with minimal disruption to cell function. These nanoelectrodes can be fabricated combined with scanning ion conductance microcopy probes which should allow high resolution electrochemical mapping of species on or in living cells.

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Monitoring in Real Time with a Microelectrode the Release of Reactive Oxygen and Nitrogen Species by a Single Macrophage Stimulated by its Membrane Mechanical Depolarization

Cited by 152 publications

References 59 publications

Real-time electrical detection of nitric oxide in biological systems with sub-nanomolar sensitivity

Real-time electrical detection of nitric oxide in biological systems with sub-nanomolar sensitivity

Redox regulation of calcium ion channels: Chemical and physiological aspects

Electrochemical Nanoprobes for Single-Cell Analysis

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