Monitoring gene expression along pear fruit development, ripening and senescence using cDNA microarrays

Fonseca, Sandra; Hackler, László; Zvara, Ágnes; Ferreira, Sı́lvia; Baldé, Aladje; Dudits, Dénes; Pais, M. S.; Puskás, László G.

doi:10.1016/j.plantsci.2004.03.033

Cited by 68 publications

(35 citation statements)

References 49 publications

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“…Such an approach has proven successful in the study of ripeningassociated processes in other fruit including strawberry (Fragaria x ananassa; Aharoni and O'Connell, 2002), pear (Pyrus communis; Fonseca et al, 2004), and tomato (Solanum lycopersicum; Alba et al, 2005). Other genomic studies of aroma biosynthesis in apple have included EST annotation of putative biosynthetic genes (Newcomb et al, 2006) and statistical and reverse transcription (RT)-PCR analysis or their tissue and developmental expression (Park et al, 2006).…”

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confidence: 99%

A Genomics Approach Reveals That Aroma Production in Apple Is Controlled by Ethylene Predominantly at the Final Step in Each Biosynthetic Pathway

et al. 2007

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Ethylene is the major effector of ripening in many fleshy fruits. In apples (Malus x domestica) the addition of ethylene causes a climacteric burst of respiration, an increase in aroma, and softening of the flesh. We have generated a transgenic line of 'Royal Gala' apple that produces no detectable levels of ethylene using antisense ACC OXIDASE, resulting in apples with no ethyleneinduced ripening attributes. In response to external ethylene these antisense fruits undergo a normal climacteric burst and produced increasing concentrations of ester, polypropanoid, and terpene volatile compounds over an 8-d period. A total of 186 candidate genes that might be involved in the production of these compounds were mined from expressed sequence tags databases and full sequence obtained. Expression patterns of 179 of these were assessed using a 15,720 oligonucleotide apple microarray. Based on sequence similarity and gene expression patterns we identified 17 candidate genes that are likely to be ethylene control points for aroma production in apple. While many of the biosynthetic steps in these pathways were represented by gene families containing two or more genes, expression patterns revealed that only a single member is typically regulated by ethylene. Only certain points within the aroma biosynthesis pathways were regulated by ethylene. Often the first step, and in all pathways the last steps, contained enzymes that were ethylene regulated. This analysis suggests that the initial and final enzymatic steps with the biosynthetic pathways are important transcriptional regulation points for aroma production in apple.

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A Genomics Approach Reveals That Aroma Production in Apple Is Controlled by Ethylene Predominantly at the Final Step in Each Biosynthetic Pathway

et al. 2007

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“…To be able to compare our results with those obtained by Fonseca and coworkers (Fonseca et al 2004), a complete linkage hierarchical clustering (Euclidean distance) was chosen.…”

Section: Methodsmentioning

confidence: 99%

“…Since our main interest was fruit quality, we focused our study on the identification of genes differentially expressed during fruit development. A similar approach has been successfully applied in peach (Trainotti et al 2006) and in pear (Fonseca et al 2004), two climacteric fruits, and in citrus, a non-climacteric fruit (Cercós et al 2006). The ultimate goal of our work was the identification of genes responsible for QTLs controlling fruit quality traits and to provide a general overview of the major metabolic processes occurring during fruit development.…”

Section: Introductionmentioning

confidence: 99%

Transcription analysis of apple fruit development using cDNA microarrays

Soglio

Costa

Molthoff³

et al. 2009

Tree Genetics & Genomes

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The knowledge of the molecular mechanisms underlying fruit quality traits is fundamental to devise efficient marker-assisted selection strategies and to improve apple breeding. In this study, cDNA microarray technology was used to identify genes whose expression changes during fruit development and maturation thus potentially involved in fruit quality traits. The expression profile of 1,536 transcripts was analysed by microarray hybridisation.

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“…Total RNA extraction and mRNA isolation Total RNA from 'Zaosu' and 'Zaosu Red' pears was extracted using the SDS-phenol method according to Fonseca et al (2004), and the RNA concentration and quality were detected by UV spectrophotometry and by running on a 1% agar/EB gel, respectively (Fig. 2).…”

Section: Plant Materialsmentioning

confidence: 99%

“…Total RNA from 'Zaosu Red' pear was extracted using the SDS-phenol method according to Fonseca et al (2004), cDNA was synthesized with PrimeScript † RT Reagent Kit (TaKaRa, Japan). The PCR amplified product was cloned using pGEM-T Easy Vector System (Promega, US), according to the protocol provided by the manufacturer.…”

Section: Sequencing and Blast Analysismentioning

confidence: 99%

A suppression subtractive hybridization library construction for red skin mutant of ‘Zaosu’ pear and MYBR gene analysis

et al. 2012

New Zealand Journal of Crop and Horticultural Science

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Red pear breeding is one of the major domestic and international pear breeding projects. The aim of this study is to investigate red skin development-related genes of the red skin mutant of 'Zaosu' (Pyrus bretschneideri Rehd.) pear and provide important information for elucidating the molecular mechanism of red pigment formation in the skin. A cDNA library was constructed using the suppression subtractive hybridization (SSH) method, in which the green-skinned pear 'Zaosu' served as the driver, and its red skin mutant 'Zaosu Red' served as the tester. All positive clones between 250Á1000 bp were chosen and sequenced from the library, and 735 expressed sequence tags (ESTs) were obtained. According to BLAST screening and functional annotation in the GenBank database, 314 unique gene fragments were obtained. Among the obtained fragments, 198 ESTs were found to have homologous genes with known functions, 61 ESTs with unknown functions and 55 ESTs with no homologous genes. Results showed that the differentially expressed genes are involved in anthocyanin biosynthesis, signal transduction, energy metabolism, transcription, disease resistance and bud mutation. Moreover, a novel cDNA of 'Zaosu Red' MYBR gene was isolated with the strategy of in silico cloning in combination with RT-PCR from 'Zaosu Red' pear fruit. The full-length of MYBR cDNA was 1552 bp, with an open reading frame from 12 to 1355 bp, coded 447 amino acids.

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Monitoring gene expression along pear fruit development, ripening and senescence using cDNA microarrays

Cited by 68 publications

References 49 publications

A Genomics Approach Reveals That Aroma Production in Apple Is Controlled by Ethylene Predominantly at the Final Step in Each Biosynthetic Pathway

A Genomics Approach Reveals That Aroma Production in Apple Is Controlled by Ethylene Predominantly at the Final Step in Each Biosynthetic Pathway

Transcription analysis of apple fruit development using cDNA microarrays

A suppression subtractive hybridization library construction for red skin mutant of ‘Zaosu’ pear and MYBR gene analysis

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