Monitoring disease activity in pemphigus with enzyme-linked immunosorbent assay using recombinant desmogleins 1 and 3

Cheng, Steven; Kobayashi, Masakazu; Tanikawa, Akiko; Kinoshita-Kuroda, K.; Amagai, Masayuki; Nishikawa, Takeji

doi:10.1046/j.1365-2133.2002.04838.x

Cited by 148 publications

(139 citation statements)

References 10 publications

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“…Observations have been made by investigators demonstrating that the titer of antiDsg-3 antibodies do not correlate with disease severity and activity. [11][12][13] A speculative explanation for the positivity of anti-Dsg-3 antibody in the absence of disease is that the antibody may be non-pathogenic. 14 A modified assay may be …”

Section: Discussionmentioning

confidence: 99%

“…needed to detect non-pathogenic epitopes. 12,13 Mignogna et al have demonstrated that adjuvant TAC injections can help reduce the systemic dose of corticosteroids. 3 This case is unique in that the patient was treated with only sublesional TAC injections and topical rinses without any systemic therapy and was able to achieve a prolonged and sustained clinical remission.…”

Section: Figurementioning

confidence: 99%

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Pemphigus vulgaris localized to the tongue

Nguyen¹,

Ahmed²

2014

J Dermatol Case Rep

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Background: Pemphigus vulgaris is an autoimmune blistering disease that may initially present as localized lesions. It rarely remains localized throughout its clinical course. Observations:A 53-year-old woman with non-progressive pemphigus vulgaris localized to the tongue for 18 years is presented. Clinical examination showed erosions and ulcerations limited to the lateral margins of the tongue. Patient was treated with sublesional triamcinolone-acetonide injections as lesions recurred. Finally, triamcinolone-acetonide injections at three weeks intervals for three months induced a longterm sustained clinical remission for 18 months. The indirect immunofluorescence did not correlate with disease activity. Anti-desmoglein 3 antibodies (ELISA) remained elevated throughout the clinical course and during remission. Conclusions:This case highlights the recognition of localized pemphigus vulgaris and demonstrates the importance of local therapy and its potential to induce longterm remission. Similar report of additional cases may create a standard of care for

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Section: Discussionmentioning

confidence: 99%

Section: Figurementioning

confidence: 99%

Pemphigus vulgaris localized to the tongue

Nguyen¹,

Ahmed²

2014

J Dermatol Case Rep

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“…Bu nedenle, takipte ve araştırma amacıyla ELISA kullanılacaksa gerçek indeks değeri için, sonuç >150 IU/ml ise dilüsyon yapılması önerilebilir (44).…”

Section: İndirekt İmmünofloresan Mozaik Tabanlı Biyoçipunclassified

Serological Diagnosis of Autoimmune Blistering Diseases

Özkesici¹,

Karakaş²

2016

tdd

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“…Bunun yan›nda desmoglein de¤erleri azalmakla birlikte yüksek seyreden 6 hastan›n 3'ünde remisyon elde edilmifltir. Bu durum enzim reaksiyonu esas›na dayanan ELISA yönteminde yüksek titrede antikor yo¤unlu¤u içeren serumlarda enzimin doygunlu¤a ulaflmas›ndan dolay› desmoglein de¤erleri-nin beklenenden daha düflük saptanmas›na ba¤l› olabilir 37,38 . ‹leri dilüsyonlar ile ayn› serumlar çal›fl›ld›¤› takdirde aktif dönem ve remisyonda desmoglein de¤erleri aras›nda saptanan az fark›n daha belirgin hale gelece¤i düflünülebilir.…”

Section: Tart›flmaunclassified

Pemfigusta Desmoglein Antikor Serum Düzeyleri ile Direkt İmmünofloresan Bulgularının Hastalığın Klinik Aktivitesi ile İlişkisi

Yilmaz¹,

Başkan²,

Budak³

et al. 2011

Turkderm

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ÖzetAmaç: Pemfigus deri ve mukozalarda bül oluflumuyla seyreden, otoimmün bir hastal›kt›r. Bu çal›flmada bül oluflumunda rolü olan desmoglein-1 (dsg-1) ve desmoglein-3 (dsg-3)'e karfl› oluflmufl antikorlar›n saptanmas›nda kullan›lan iki yöntem olan direkt immün-floresan (IF) inceleme ve ELISA yönteminin hastal›k aktivitesi ve remisyonla iliflkisi araflt›r›lmaktad›r. Gereç ve Yöntem: Çal›flmaya 23'ü pemfigus vulgaris, 2'si pemfigus foliaseus tan›s› alm›fl toplam 25 hasta al›nd›. Hastalar›n tedavi öncesi ve klinik remisyonun 3., 6. ve 12. aylar›ndaki anti-dsg-1 ve anti-dsg-3 serum antikor düzeyleri ELISA ile araflt›r›ld›. Efl zamanl› olarak aktif hastal›kta lezyon kenar›ndan, remisyonda ise sa¤lam kalça derisi/alt dudak mukozas›ndan direkt IF inceleme yap›ld›. Nüks halinde tetkikler tekrarland›. Bulgular: Pemfigus vulgaris hastalar›n›n tedavi öncesi 17'sinde (%73,9) anti-dsg-1 antikoru, hepsinde (%100) anti-dsg-3 antikoru pozitif saptand›. ‹ki pemfigus foliaseus olgusunda tedavi öncesi anti-dsg-1 pozitif de¤erlerde iken anti-dsg-3 negatif saptand›. Antidsg-1 antikor serum düzeyleri deri fliddet skoru ile (r: 0,577; p: 0,003), anti-dsg-3 antikor serum düzeyleri ise oral mukoza fliddet skoru ile korele idi (r: 0,539; p: 0,008). Tam remisyona giren hastalar›n 16's›nda (%84,2) tedavi öncesi direkt IF'da saptanan birikim remisyonla birlikte negatifleflti. Nüks gözlenen 9 hastan›n hepsinde nüks s›ras›nda anti-dsg-1 ve/veya anti-dsg-3 serum düzeylerinde art›fl saptand›. Dokuz olgunun 3'ünde ise klinik remisyon halinde iken nüksten 1-4 ay öncesinde serum antikor düzeylerinde yük-selme tespit edildi. Sonuç: Bu çal›flmada serum desmoglein otoantikor de¤erlerinin hastal›k fliddeti ve aktivitesi ile iliflkili olabilece¤ini saptad›k. Klinik remisyon esnas›nda desmoglein antikorlar›n›n seri ölçümleri takip ve tedavi modifikasyonunda yol gösterici olabilir. (Türkderm 2011; 45: 77-82) Anahtar Kelimeler: Pemfigus vulgaris, pemfigus foliaseus, direkt immünfloresan, desmoglein antikorlar›, hastal›k aktivitesi Summary Background and Design: Pemphigus is an autoimmune disease that results in blistering of the skin and mucous membranes. In this study, we investigated the relationship between disease activity and remission with ELISA scores and direct immunofluorescence (IF) -two methods used for the detection of antibodies against desmoglein-1 (dsg-1) and desmoglein-3 (dsg-3) that are responsible for blister formation. Material and Method: Twenty-three pemphigus vulgaris patients and two pemphigus foliaceus patients were enrolled in the study. The serum levels of anti-dsg-1 and anti-dsg-3 antibodies were measured with ELISA before therapy and at 3, 6, and 12 month of clinical remission. Concurrently, direct IF was performed on perilesional skin during active disease and on normal buttock skin/lower lip mucosa in remission. The tests were repeated if relapse has occured. Results: Anti-dsg-1 was detected in 17 (73.9%) pemphigus vulgaris patients and anti-dsg-3 in 23 (100%) pemphigus vulgaris patients. In two pemphigus foliaceus patient...

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Monitoring disease activity in pemphigus with enzyme-linked immunosorbent assay using recombinant desmogleins 1 and 3

Abstract: These findings indicate that when appropriate dilutions are used in Dsg1 and Dsg3 ELISA, these assays can provide useful serological information for assessing disease activity in PF and PV.

Cited by 148 publications

References 10 publications

Pemphigus vulgaris localized to the tongue

Pemphigus vulgaris localized to the tongue

Serological Diagnosis of Autoimmune Blistering Diseases

Pemfigusta Desmoglein Antikor Serum Düzeyleri ile Direkt İmmünofloresan Bulgularının Hastalığın Klinik Aktivitesi ile İlişkisi

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