Monitoring dioxins in food and feedstuffs using accelerated solvent extraction with a novel integrated carbon fractionation cell in combination with a CAFLUX bioassay

Abstract

The concentrations of dioxins in fish oil and fish meal were determined with accelerated solvent extraction, using a novel integrated carbon fractionation extraction cell followed by a miniturized multilayer silica column and bioanalysis on a recently-developed chemically-activated fluorescent gene expression cell bioassay. The developed method allows for simultaneous gravimetric lipid weight determination, which was shown for both matrices under study (about 100% lipid recovery of each sample). Initial results practically meet the quality criteria on screening methods for control of dioxins in food and feedstuffs laid down in the EU Commission Directives 2002/69/EC (food) and 2002/70/EC (feed). This demonstrates that the developed method can be used as a screening tool for monitoring dioxins in food and feed after some additional improvements and testing on a greater number of matrices.

Analysis of dioxins in contaminated soils with the CALUX and CAFLUX bioassays, an immunoassay, and gas chromatography/high‐resolution mass spectrometry

“…We have therefore developed a simultaneous extraction and fractionation technique for PCDD/Fs in contaminated soils with the use of selective accelerated solvent extraction (ASE), and it has been successfully combined with the ELISA detection method [28]. A similar selective ASE method has been developed and used in combination with CAFLUX for determining dioxin TEQs in food and feed samples [38]. Currently, the performance of selective ASE of soil samples with CAFLUX detection is being evaluated, and a significant reduction in costs and increase in throughput is expected.…”

Advances in analytical techniques for polychlorinated dibenzo-p-dioxins, polychlorinated dibenzofurans and dioxin-like PCBs

“…Van Beuzekom used an automated microwave solvent extraction system (MASE) coupled to a liquid chromatograph for the analysis of fine particles from fire emissions [59]. Nording [72] was able to modify a PLE extraction cell to insert the sample matrix above AX21 carbon packing in order to perform extraction and clean-up in the cell as a screening method. Results compared well with GC/HRMS confirmatory analysis.Sample preparation schemes are developed to remove most interferences from the sample extract.…”

Coupling passive sampling with in vitro bioassays and chemical analysis to understand combined effects of bioaccumulative chemicals in blood of marine turtles

“…There was no statistically significant 3. Comparison of (A) AhR-and (B) Nrf2-active chemical burdens in marine wildlife species, including green turtles (this study) and dugongs (Jin et al, 2015) from Australia, Baikal seals from Russia and finless porpoises from Japan (Suzuki et al, 2011), polar bears from East Greenland (Erdmann et al, 2013), North Sea herring from Sweden (Nording et al, 2005) and Atlantic cod from Poland (Dabrowska et al, 2010). Left, middle and right lines represent the minimum, median and maximum values, respectively.…”