Monitoring deep brain stimulation by measuring regional brain oxygen responses in freely moving mice

Bazzu, Gianfranco; Serra, Pier Andrea; Hamelink, Ralph; Feenstra, Matthijs G.P.; Willuhn, Ingo; Denys, Damiaan

doi:10.1016/j.jneumeth.2019.01.012

Cited by 2 publications

(1 citation statement)

References 44 publications

(47 reference statements)

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“…Basal oxygen concentrations recorded in the extracellular space of the striatum of freely-moving rats resulted being relatively low (35–50 μM) [9–11] similar to those recorded in the same brain region of mice (~30 μM) [25]. Surprisingly, these observations are in agreement with the results of the present study.…”

Section: Discussionsupporting

confidence: 92%

Real-time telemetry monitoring of oxygen in the central complex of freely-walking Gromphadorhina portentosa

et al. 2019

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A new telemetric system for the electrochemical monitoring of dissolved oxygen is showed. The device, connected with two amperometric sensors, has been successfully applied to the wireless detection of the extracellular oxygen in the central complex of freely-walking Gromphadorhina portentosa. The unit was composed of a potentiostat, a two-channel sensor conditioning circuit, a microprocessor module, and a wireless serial transceiver. The amperometric signals were digitalized and sent to a notebook using a 2.4 GHz transceiver while a serial-to-USB converter was connected to a second transceiver for completing the communication bridge. The software, running on the laptop, allowed to save and graph the oxygen signals. The electronics showed excellent stability and the acquired data was linear in a range comprised between 0 and -165 nA, covering the entire range of oxygen concentrations. A series of experiments were performed to explore the dynamics of dissolved oxygen by exposing the animals to different gases (nitrogen, oxygen and carbon dioxide), to low temperature and anesthetic agents (chloroform and triethylamine). The resulting data are in agreement with previous O2 changes recorded in the brain of awake rats and mice. The proposed system, based on simple and inexpensive components, can constitute a new experimental model for the exploration of central complex neurochemistry and it can also work with oxidizing sensors and amperometric biosensors.

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Section: Discussionsupporting

confidence: 92%

Real-time telemetry monitoring of oxygen in the central complex of freely-walking Gromphadorhina portentosa

et al. 2019

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Anesthesia and analgesia for experimental craniotomy in mice and rats: a systematic scoping review comparing the years 2009 and 2019

et al. 2023

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Experimental craniotomies are a common surgical procedure in neuroscience. Because inadequate analgesia appears to be a problem in animal-based research, we conducted this review and collected information on management of craniotomy-associated pain in laboratory mice and rats. A comprehensive search and screening resulted in the identification of 2235 studies, published in 2009 and 2019, describing craniotomy in mice and/or rats. While key features were extracted from all studies, detailed information was extracted from a random subset of 100 studies/year. Reporting of perioperative analgesia increased from 2009 to 2019. However, the majority of studies from both years did not report pharmacologic pain management. Moreover, reporting of multimodal treatments remained at a low level, and monotherapeutic approaches were more common. Among drug groups, reporting of pre- and postoperative administration of non-steroidal anti-inflammatory drugs, opioids, and local anesthetics in 2019 exceeded that of 2009. In summary, these results suggest that inadequate analgesia and oligoanalgesia are persistent issues associated with experimental intracranial surgery. This underscores the need for intensified training of those working with laboratory rodents subjected to craniotomies.Systematic review registrationhttps://osf.io/7d4qe.

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Monitoring deep brain stimulation by measuring regional brain oxygen responses in freely moving mice

Cited by 2 publications

References 44 publications

Real-time telemetry monitoring of oxygen in the central complex of freely-walking Gromphadorhina portentosa

Real-time telemetry monitoring of oxygen in the central complex of freely-walking Gromphadorhina portentosa

Anesthesia and analgesia for experimental craniotomy in mice and rats: a systematic scoping review comparing the years 2009 and 2019

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