Monitoring cells local temperature variation using nitrogen-vacancy (NV) centers in nanodiamonds

Fluorescent Nanodiamonds for Nanoscale Thermometry in Biology

“…It indicates that the absolute temperate determination from a single nanodiamond measurement can be problematic due to the natural variation of nanocrystal properties. A similar point was addressed in [32], where ND were tested in different solutions (Figure 3b). Minor variations of the temperature dependence of ODMR shift and, more importantly, its statistical distribution also demonstrated that absolute temperature can hardly be found from a single spot measurement.…”

“…Fluorescent ND provide the outstanding possibility to measure temperature, especially intercellular temperature, independently from the environment [32], which is impossible for other currently used thermometers based on fluorescent nanoparticles [2,6,9]. The linear dependences of optical properties of various diamond color centers (ZPL or ODMR) allow for measuring absolute changes of variable temperature [20,[27][28][29]44,50,51].…”

“…The measured temperature increase was not biologically related but was made and controlled by adding gold nanoparticles to convert light into heat. It was proof-of-principle experiments and became the start of many following works [30][31][32].…”

Toward a holographic brain paradigm: a lipid-centric model of brain functioning

“…The permanent phospholipid dipoles are very sensitive to thermal excitation and EMFs; the former randomizes dipoles, while the latter align them (Lisi et al, 2006). The development of sensors composed of quantum defects within a diamond chip enabled precise measurements of the magnetic field correlated to an individual neuron's AP (Petrini et al, 2022). Based on the thermodynamic properties of neuronal membranes, including volume and area changes and ion dynamics, APs can be described as a mechanicallycoupled EM events rather than propagating voltage gradients (Wu et al, 2019).…”

“…Indeed, it is the best-positioned mechanism for the brain to accomplish what it does within limited space and metabolic energy usage (Petrini et al, 2022).…”