Monitoring and molecular characterization of bacterial species in heavy metals contaminated roadside soil of selected region along NH 8A, Gujarat

Singh, Snigdha; Hiranmai, R. Y.

doi:10.1016/j.heliyon.2021.e08284

Cited by 9 publications

(4 citation statements)

References 40 publications

(38 reference statements)

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“…The Pseudomonas genus can grow and develop under unfavorable conditions, metabolize a wide variety of organic and inorganic compounds, as well as reduce and volatilize heavy metals ( Beltrán-Pineda and Gómez-Rodríguez, 2016 ). SAICEUPSM T strain has functional genes related to the reduction and volatilization of mercury, resistance to copper, cobalt, zinc, cadmium, as well as siderophores production capacity that help improve tolerance or transform ions of toxic metals, minimizing their toxic effects ( Singh and Hiranmai, 2021 ), which could justify the high resistance values obtained in laboratory tests. There are few bibliographical references that review the tolerance of different species of the Pseudomonas genus to mercury.…”

Section: Discussionmentioning

confidence: 99%

Oxidative stress protection and growth promotion activity of Pseudomonas mercuritolerans sp. nov., in forage plants under mercury abiotic stress conditions

et al. 2022

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SAICEUPSMT strain was isolated from soils in the mining district of Almadén (Ciudad Real, Spain), subjected to a high concentration of mercury. Using the plant model of lupinus, the strain was inoculated into the rhizosphere of the plant in a soil characterized by a high concentration of mercury (1,710 ppm) from an abandoned dump in the mining district of Almadén (Ciudad Real, Spain). As a control, a soil with a minimum natural concentration of mercury, from a surrounding area, was used. Under greenhouse conditions, the effect that the inoculum of the SAICEUPSMT strain had on the antioxidant capacity of the plant was studied, through the quantification of the enzymatic activity catalase (CAT), ascorbate peroxidase (APX), superoxide dismutase (SOD), and glutathione reductase (GR). Likewise, the capacity of the plant to bioaccumulate mercury in the presence of the inoculum was studied, as well as the effect on the biometric parameters total weight (g), shoot weight (g), root weight (g), shoot length (cm), root length (cm), total number of leaves (N), and total number of secondary roots (No). Finally, in view of the results, the SAICEUPSMT strain was identified from the phenotypic and genotypic point of view (housekeeping genes and complete genome sequencing). The inoculum with the SAICEUPSMT strain in the presence of mercury produced a significant reduction in the enzymatic response to oxidative stress (CAT, APX, and SOD). It can be considered that the strain exerts a phytoprotective effect on the plant. This led to a significant increase in the biometric parameters total plant weight, root weight and the number of leaves under mercury stress, compared to the control without abiotic stress. When analyzing the mercury content of the plant with and without bacterial inoculum, it was found that the incorporation of the SAICEUPSMT strain significantly reduced the uptake of mercury by the plant, while favoring its development in terms of biomass. Given the positive impact of the SAICEUPSMT strain on the integral development of the plant, it was identified, proving to be a Gram negative bacillus, in vitro producer of siderophores, auxins and molecules that inhibit stress precursors. The most represented fatty acids were C16:0 (33.29%), characteristic aggregate 3 (22.80%) comprising C16:1 ω7c and C16: 1ω6c, characteristic aggregate 8 (13.66%) comprising C18:1 ω7c, and C18: 1 cycle ω6c and C 17:0 (11.42%). From the genotypic point of view, the initial identification of the strain based on the 16S rRNA gene sequence classified it as Pseudomonas iranensis. However, genome-wide analysis showed that average nucleotide identity (ANI, 95.47%), DNA-DNA in silico hybridization (dDDH, 61.9%), average amino acid identity (AAI, 97.13%), TETRA (0.99%) and intergenic distance (0.04) values were below the established thresholds for differentiation. The results of the genomic analysis together with the differences in the phenotypic characteristics and the phylogenetic and chemotaxonomic analysis support the proposal of the SAICEUPSMT strain as the type strain of a new species for which the name Pseudomonas mercuritolerans sp. is proposed. No virulence genes or transmissible resistance mechanisms have been identified, which reveals its safety for agronomic uses, under mercury stress conditions.

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Section: Discussionmentioning

confidence: 99%

Oxidative stress protection and growth promotion activity of Pseudomonas mercuritolerans sp. nov., in forage plants under mercury abiotic stress conditions

et al. 2022

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“…Pb-resistant indigenous bacteria have been successfully isolated from soil in a horticultural area that applies intensive farming and the total population was 6.39 x 10 6 CFU/g (Table 1). Previously Singh & Hiranmai (2021) reported that bacteria population in soil from different roadsides containing Pb >70 mg/kg is around 10 5 -10 6 CFU/g. The isolated Pbresistant are indigenous (local) bacteria that benefit the environment (Kumar & Gopal 2015) growth-promoting (PGP) traits to increase soil fertility (Kumar & Gopal 2015;Bhat et al 2022).…”

Section: Pb-resistant Bacteria Populationmentioning

confidence: 99%

Lead (Pb)-Resistant Bacteria Improve <i>Brassica chinensis</i> Biomass and Reduce Pb Concentration in Pb-Contaminated Soil

Pertiwi,

Ustiatik,

Nuraini

2024

J.Tropical Biodiversity Biotechnology

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Applications of inorganic fertilisers and pesticides frequently increase lead (Pb) content in the soil and food crops. This study aims to isolate Pb-resistant bacteria and test the isolated bacteria in reducing Pb concentration and increasing biomass production of Brassica chinensis on Pb-contaminated soil. Soil and plant samples were collected from agricultural land in Batu City, East Java, Indonesia. The isolated bacteria were tested for Pb resistance and then characterised according to 16S rRNA Sequence. A pot trial with a completely randomised block design consisting of 9 treatments and 3 replications was set to determine the effect of Pb-resistant bacteria inoculation on Pb residue, plant growth, and soil nutrients. The result showed that the isolated Pb-resistant bacteria were Bacillus wiedmannii and Bacillus altitudinis. The bacteria were resistant to Pb up to 10,000 mg/L PbNO3. Inoculation of the bacteria increased B. chinensis growth and biomass production, namely increasing the number of leaves (12%) and dry weight (35%). Also, the bacteria reduced Pb residue in the soil by up to 88%. Moreover, soil essential nutrients such as total nitrogen, available phosphorus, and exchangeable potassium increased (12%, 73%, and 200%, respectively) after the application of Pb-resistant bacteria. The bacteria have the potential for bioremediation of Pb-contaminated soils on a large scale due to the bacteria prevent Pb uptake by food crops such as B. chinensis by reducing Pb content in the soil, which is good for food safety and environmental sustainability.

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“…Some researchers reported that M. luteus, S. maltophilia, and Brevundimonas sp. were known to have high resistance to heavy metals, including Cr(VI) (Marzan et al 2017;Singh and Hiranmai 2021). According to Abamhekhelu et al (2019), Micrococcus sp.…”

Section: Molecular and Phylogenic Analysis Of Cr(vi)-resistant Bacteriamentioning

confidence: 99%

Isolation and identification of Cr-resistant bacteria from the rhizosphere of Tagetes sp. and their ability to reduce Cr(VI)

Meitiniarti

PUTRI²,

RUNTU³

et al. 2022

Biodiversitas

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Abstract. Meitiniarti VI, Putri EK, Runtu AE, Nugroho RA, Kasmiyati S. 2022. Isolation and identification of Cr-resistant bacteria from the rhizosphere of Tagetes sp. and their ability to reduce Cr(VI). Biodiversitas 23: 4118-4124. Bioremediation involving the interaction of plants with microorganisms is a technique for improving the environment polluted with chromium hexavalent [Cr(VI)]. Marigolds (Tagetes sp.) are known to be a hyperaccumulator plant for Cr(VI) and there are microbes in its rhizosphere that enable the plant tolerant to heavy metals. The aims of this study were to isolate and identify indigenous rhizobacteria resistant to Cr(VI) from Tagetes sp. and to test the ability of these bacterial isolates to reduce Cr(VI). Three isolates of Cr(VI) resistant bacteria were obtained in this study, namely Micrococcus luteus RT-9, Stenotrophomonas maltophilia RT-12, and Brevundimonas sp. RT-23. M. luteus RT-9 and S. maltophilia RT-12 which were resistant to Cr(VI) 100 mg L-1 and their mixture were tested for their ability to reduce Cr(VI) in a liquid medium. Microbacterium sp. SpR3 obtained by the previous study was used as a reference. The results showed that the isolate of S. maltophilia RT-12 had a higher ability (2.2 mg L-1 h-1) than M. luteus RT-9 (0.9 mg L-1 h-1). S. maltophilia RT-12 had the ability to reduce Cr(VI) similar to Microbacterium sp. SpR3. The mixed inoculation of M. luteus RT-9, S. maltophilia RT-12, and Microbacterium sp. SpR3 did not show a difference in the reduction ability of Cr (VI) compared to pure cultures.

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Monitoring and molecular characterization of bacterial species in heavy metals contaminated roadside soil of selected region along NH 8A, Gujarat

Cited by 9 publications

References 40 publications

Oxidative stress protection and growth promotion activity of Pseudomonas mercuritolerans sp. nov., in forage plants under mercury abiotic stress conditions

Oxidative stress protection and growth promotion activity of Pseudomonas mercuritolerans sp. nov., in forage plants under mercury abiotic stress conditions

Lead (Pb)-Resistant Bacteria Improve <i>Brassica chinensis</i> Biomass and Reduce Pb Concentration in Pb-Contaminated Soil

Isolation and identification of Cr-resistant bacteria from the rhizosphere of Tagetes sp. and their ability to reduce Cr(VI)

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