Moments of Inertia

Hughes, Jennifer; Martin, K. F.

doi:10.1007/978-1-349-02449-0_12

Cited by 2 publications

(2 citation statements)

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“…The resultant data table and labeled filament image volume were saved for further analyses. The moment of inertia along the longest principal axis (I3) was then divided by the volume for each myosin filament to determine its deviation from linearity 74 by using a custom ImageJ macro to perform math operations within results tables. Visualization of filament deviation from linearity values was performed by using the Assign Measure to Label function within the MorphoLibJ 75 plugin (Plugins-MorphoLibJ-Label Images) using the labeled filament image and the I3/volume values from the results table.…”

Section: Methodsmentioning

confidence: 99%

Mitochondrial Network Configuration Influences Sarcomere and Myosin Filament Structure in Striated Muscles

Katti

Hall

Ajayi

et al. 2022

Preprint

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Sustained muscle contraction occurs through interactions between actin and myosin filaments within sarcomeres and requires a constant supply of adenosine triphosphate (ATP) from nearby mitochondria. However, it remains unclear how different physical configurations between sarcomeres and mitochondria alter the energetic support for contractile function. Here, we show that sarcomere cross-sectional area (CSA) varies along its length in a cell type-dependent manner where the reduction in Z-disk CSA relative to the sarcomere center is closely coordinated with mitochondrial network configuration. Further, we find myosin filaments near the sarcomere periphery are curved relative to interior filaments with greater curvature for filaments near mitochondria compared to the sarcoplasmic reticulum. Finally, we demonstrate myosin filament lattice spacing is smaller at filament ends than filament centers in a cell type-dependent manner. These data suggest that both sarcomere structure and myofilament interactions are influenced by the location and orientation of mitochondria within a muscle cell.

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Section: Methodsmentioning

confidence: 99%

Mitochondrial Network Configuration Influences Sarcomere and Myosin Filament Structure in Striated Muscles

Katti

Hall

Ajayi

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

“…The resultant data table and labeled filament image volume were saved for further analyses. The moment of inertia along the longest principal axis (I3) was then divided by the volume for each myosin filament to determine its deviation from linearity 115 by using a custom ImageJ macro to perform math operations within results tables. Visualization of filament deviation from linearity values was performed by using the Assign Measure to Label function within the MorphoLibJ 116 plugin (Plugins-MorphoLibJ-Label Images) using the labeled filament image and the I3/volume values from the results table.…”

Section: Image Analysismentioning

confidence: 99%

Mitochondrial network configuration influences sarcomere and myosin filament structure in striated muscles

et al. 2022

View full text Add to dashboard Cite

Sustained muscle contraction occurs through interactions between actin and myosin filaments within sarcomeres and requires a constant supply of adenosine triphosphate (ATP) from nearby mitochondria. However, it remains unclear how different physical configurations between sarcomeres and mitochondria alter the energetic support for contractile function. Here, we show that sarcomere cross-sectional area (CSA) varies along its length in a cell type-dependent manner where the reduction in Z-disk CSA relative to the sarcomere center is closely coordinated with mitochondrial network configuration in flies, mice, and humans. Further, we find myosin filaments near the sarcomere periphery are curved relative to interior filaments with greater curvature for filaments near mitochondria compared to sarcoplasmic reticulum. Finally, we demonstrate variable myosin filament lattice spacing between filament ends and filament centers in a cell type-dependent manner. These data suggest both sarcomere structure and myofilament interactions are influenced by the location and orientation of mitochondria within muscle cells.

show abstract

Moments of Inertia

Cited by 2 publications

References 0 publications

Mitochondrial Network Configuration Influences Sarcomere and Myosin Filament Structure in Striated Muscles

Mitochondrial Network Configuration Influences Sarcomere and Myosin Filament Structure in Striated Muscles

Mitochondrial network configuration influences sarcomere and myosin filament structure in striated muscles

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