Molybdenum Reduction to Molybdenum Blue in<i>Serratia</i>sp. Strain DRY5 Is Catalyzed by a Novel Molybdenum-Reducing Enzyme

Shukor, Mohd Yunus; Halmi, Mohd Izuan Effendi; Rahman, Mohd Fadhil; Shamaan, Nor Aripin; Syed, Mohd Arif

doi:10.1155/2014/853084

Cited by 21 publications

(39 citation statements)

References 34 publications

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“…This is similar to all isolated Mo-reducing bacteria that have been reported, exhibiting optimal conditions from the lowest pH at 5.0 to the highest pH at 7.0 [15,[29][30][31][32][33][34]. As the stability and formation of phosphomolybdate are optimal under acidic conditions, determination of optimal pH plays a significant role.…”

Section: Optimum Temperature and Ph On Molybdenum Reductionsupporting

confidence: 83%

“…Based on previous studies, the phosphate concentration required is around 5 mM and lower for optimal Mo-reduction [13,15,18,23,28,[30][31][32][33][34][35][36][37]39]. The result of the present study showed that maximum concentration of phosphate also occurred at 5.0 mM.…”

Section: Effect Of Phosphate and Molybdate Concentration On Moreductionsupporting

confidence: 58%

“…Incubation was carried out for 48 hours via continuous orbital shaking at a speed of 120 rpm. The high phosphate concentrations prevented the production of blue cellular aggregates that complicate cell harvesting, but the Mo-reducing activity was retained [13][14][15].…”

Section: Isolation Of Molybdenum-reducing Bacteriummentioning

confidence: 99%

“…The broad optimum conditions exhibited by the bacterium fit exceptionally well to a tropical country like Malaysia having 25 to 35°C as the yearly average temperature [28]. The majority of molybdenum reducers are isolated from tropical soils and display optimal reduction between 25 and 37 ºC [15,[29][30][31][32][33] with the exception of a reducer that was isolated from Antartica. The…”

Section: Optimum Temperature and Ph On Molybdenum Reductionmentioning

confidence: 99%

“…Glucose and sucrose are easily assimilable carbon sources. They produce the reducing equivalents NADH as well as NADPH (efficient electron donors, especially for the Mo-reducing enzyme) far easier than other carbon sources [15,38] through metabolic pathways. This includes glycolysis, the citric acid cycle and the electron transport system.…”

Section: Effect Of Carbohydrate On Molybdate Reductionmentioning

confidence: 99%

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Assessing Resistance and Bioremediation Ability of Enterobacter sp. Strain Saw-1 on Molybdenum in Various Heavy Metals and Pesticides

Sabullah

Rahman

Ahmad

et al. 2017

J. Math. Fund. Sci.

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Abstract. One of the most economical approaches for removal of toxic compounds is bioremediation. In the long term, bioremediation is economic and feasible compared to other methods, such as physical or chemical methods. A bacterium that can efficiently reduce molybdenum blue was isolated from polluted soil. Biochemical analysis revealed the identity of the bacterium as Enterobacter sp. strain Saw-1. The growth parameters for optimal reduction of molybdenum to Mo-blue or molybdenum blue, a less toxic product, were determined around pH 6.0 to 6.5 and in the range of 30 to 37 ℃, respectively. Glucose was selected as preferred carbon source, followed by sucrose, maltose, l-rhamnose, cellobiose, melibiose, raffinose, d-mannose, lactose, glycerol, dadonitol, d-mannitol, l-arabinose and mucate. Phosphate and molybdate were critically required at 5.0 mM and 10 mM, respectively. The scanning absorption spectrum acquired to detect the development of complex Mo-blue showed similarity to previously isolated Mo-reducing bacteria. In addition, the spectrum closely resembled the molybdenum blue from the phosphate determination method. Heavy metals, including mercury, copper (II) and silver (I), inhibited reduction. Moreover, the bacterium also showed capability of exploiting the pesticide coumaphos as an alternative carbon source for growth. As the bacterium proved its ability to detoxify organic and inorganic xenobiotics, the usefulness of this microorganism for bioremediation is highlighted.

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Section: Optimum Temperature and Ph On Molybdenum Reductionsupporting

confidence: 83%

Section: Effect Of Phosphate and Molybdate Concentration On Moreductionsupporting

confidence: 58%

Section: Isolation Of Molybdenum-reducing Bacteriummentioning

confidence: 99%

Section: Optimum Temperature and Ph On Molybdenum Reductionmentioning

confidence: 99%

Section: Effect Of Carbohydrate On Molybdate Reductionmentioning

confidence: 99%

See 3 more Smart Citations

Assessing Resistance and Bioremediation Ability of Enterobacter sp. Strain Saw-1 on Molybdenum in Various Heavy Metals and Pesticides

Sabullah

Rahman

Ahmad

et al. 2017

J. Math. Fund. Sci.

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Characterization of a molybdenum-reducing Bacillus sp. strain khayat with the ability to grow on SDS and diesel

Khayat

Rahman

Shukor³

et al. 2016

Rend. Fis. Acc. Lincei

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Molybdenum and heavy metals are toxicants that are needed to be removed from the environment as they are non-biodegradable and pose a serious threat to the ecology. A previously isolated keratin-degrading Bacillus sp. strain khayat was shown to be able to reduce molybdenum (sodium molybdate) to molybdenum blue (Mo-blue). Reduction occurred optimally at the pHs of between 5.8 and 6.8 and temperatures of between 25 and 34 °C. Glucose was the best electron donor for supporting molybdate reduction followed by sucrose, fructose, glycogen, lactose, meso-inositol and glycerol in descending order. Other requirements include a phosphate concentration between 5.0 and 7.5 mM and a molybdate concentration of between 10 and 20 mM. The absorption spectrum of the Mo-blue produced was similar to previous Mo-reducing bacterium, and closely resembles a reduced phosphomolybdate. Molybdenum reduction was inhibited by Hg (ii), Ag (i), Cu (ii), As (v) and Pb (ii) at 84.7, 78.9, 53.5, 36.8 and 27.7 %, respectively. Analysis using phylogenetic analysis resulted in a tentative identification of the bacterium as Bacillus sp. strain khayat. The bacterium was unable utilize any of the xenobiotics as sources of electron donor for reduction but the bacterium was able to grow on diesel and SDS. The ability of this bacterium to detoxify several toxicants makes this bacterium an important tool for bioremediation of multiple toxicants.

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Modelling the kinetics of hexavalent molybdenum (Mo6+) reduction by the Serratia sp. strain MIE2 in batch culture

Halmi

Abdullah

Johari

et al. 2016

Rend. Fis. Acc. Lincei

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In the present work, the kinetics of hexavalent molybdenum reduction by the Serratia sp. strain MIE2 were investigated using several kinetic models, such as Monod, Haldane, Teissier, Aiba, Yano, Han and Levenspiel and Luong. The statistical analysis showed that the best model was Teissier, which had the lowest RMSE and AICc values, the highest adjusted R 2 values, and an F test and with a bias factor and an accuracy factor nearest to unity (1.0). The calculated value for the Teissier constants, such as p max , K s and K i , was 0.506 lmol Mo-blue h -1 , 6.53 mM and 29.41 mM, respectively. The effect of heavy metals showed that hexavalent molybdenum reduction by the strain MIE2 was inhibited by silver, mercury and copper with a total inhibition of 96, 97, and 45 %, respectively, at a concentration of 1 ppm. Otherwise, the Mo-reducing enzyme was inhibited by mercury and zinc with an inhibition of 88 and 65 %, respectively. Most of the respiratory inhibitors did not inhibit the Mo-reducing enzyme activity, indicating that the respiratory system in this bacterium is not the site of the hexavalent molybdenum reduction. The results obtained from this study could be useful for estimating the relationship between molybdenum-blue production and the molybdate concentration, which may be important during the up scaling of the molybdenum bioremediation process.

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Molybdenum Reduction to Molybdenum Blue inSerratiasp. Strain DRY5 Is Catalyzed by a Novel Molybdenum-Reducing Enzyme

Cited by 21 publications

References 34 publications

Assessing Resistance and Bioremediation Ability of Enterobacter sp. Strain Saw-1 on Molybdenum in Various Heavy Metals and Pesticides

Assessing Resistance and Bioremediation Ability of Enterobacter sp. Strain Saw-1 on Molybdenum in Various Heavy Metals and Pesticides

Characterization of a molybdenum-reducing Bacillus sp. strain khayat with the ability to grow on SDS and diesel

Modelling the kinetics of hexavalent molybdenum (Mo6+) reduction by the Serratia sp. strain MIE2 in batch culture

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