Molybdenocene–DNA interaction studies using electrochemical analysis

Water-soluble molybdenocene complexes with both proliferative and antiproliferative effects on cancer cell lines and their binding interactions with human serum albumin

“…These data are of fundamental importance because there are many oxidation processes that could result in damage to biological molecules and because not only Mo(IV) but also Mo(VI) has been shown to possess anticancer properties [45]. Furthermore, CV has been shown to be a very sensitive analytical technique to determine changes in redox behavior of metallic species in the presence of biologically important molecules [46-51]. The redox behavior of metallic species is very sensitive to the environment surrounding the metal center (solvent, ligand, charge); therefore, metal-based interaction can be detected using this technique.…”

“…2), the three Mo(IV) species exhibit irreversible electrochemical behaviors under physiological conditions. According to Rodríguez et al [51], Cp 2 MoCl 2 shows an irreversible oxidation peak at 700 mV, which is attributed to the formation of electrodeficient and reactive Mo(V) species, or the formation of a stable metal–oxo complex, which prevents the reverse reduction. The same irreversible behaviors were observed for the two molybdenocene derivatives.…”

“…Therefore, we investigated the binding interactions between Cp 2 MoCl 2 , Cp 2 Mo(malonate), and [Cp 2 Mo(maltolato)]Cl complexes and HSA under physiological conditions, monitored by UV–vis spectroscopy and CV. The latter has proved to be a sensitive tool to measure weak metal–DNA interactions [51]. …”

“…Comparison of the electrochemical behavior of the blanks and the HSA titrations allowed us to estimate binding interactions. In the past, E pa was used to evaluate the percentage of interaction between the metal and DNA, because it is more sensitive to the coordination environment than the current [51]. Table 1 presents the summary of the electrochemical data and Table S3 presents the complete titration data.…”

“…Upon addition of HSA to Cp 2 MoCl 2 , the E pa shifts toward higher potentials and the oxidation peak eventually disappears. Using the same method as for DNA [51], the maximum interaction between molybdenocene and HSA is 15%. Although the value appears to be rather small, caution must be taken.…”