Abstract:Seeds of the leguminous plant Millettia thonningii were shown to possess promising molluscicidal activity against Bulinus trunctatus. The size of the snails was an important determinant of their susceptibility to the molluscicide; specimens with shell lengths of 2 to 3 mm being more susceptible than snails 5 to 6 mm long.
“…of the cephalopedal mass (Evans et al 1986, Duncan and Sturrock 1987, Molgaard et al 2000, Al-Zanbagi et al 2001) and confirmed, if on microscopic examination, there were no observable heartbeats (Marston and Hostettmann 1985). The numbers of dead and live snails were recorded.…”
Section: Bioassay For Molluscicidal Activitymentioning
confidence: 96%
“…completely retracted into their shells (Evans et al 1986) they were discoloured (dos Santos et al 2000, Molgaard 2. et al 2000 or they were immobile and unresponsive to gentle prodding 3.…”
Section: Bioassay For Molluscicidal Activitymentioning
confidence: 98%
“…These include Phytolacca dodecandra, the best-studied plant molluscicide to date (Esser et al 2003), Millettia thonningii (Abbiw 1990) and Pavetta owariensis (Balde et al 1986, Evans et al 1986). …”
Laboratory studies were conducted to determine the potential molluscicidal activity of five Nigerian plant species used in alternative medicine practice as anthelmintics. Laboratory-bred adult Biomphalaria pfeifferi, the snail host of Schistosoma mansoni in Nigeria, and their viable 0-to 24-hour-old egg masses were separately exposed for 24 hours, in replicated bioassays, to 7.81-4 000 mg l -1 concentrations of crude ethanolic extracts of the leaves and roots of Annona senegalensis, Anogeissus leiocarpus, Crotalaria retusa, Dalbergia sissoo and Vernonia amygdalina. Fruits and stem bark of D. sissoo were also investigated. The 24-hour LC 50 and LC 90 values of extracts for target organisms were calculated using probit analysis. Susceptibility of target organisms to extracts varied with the plant species and morphological part. Egg masses and adult snails were most susceptible to D. sissoo fruit extract (LC 90 values of 89.29 and 74.33 mg l -1 , respectively) and least susceptible to V. amygdalina leaf extract (LC 90 values of 11 864. 09 and 6 241.61 mg l -1 , respectively). Concentration-dependent behavioural responses and embryo deaths at the gastrula/exogastrula and/or prehatch snail stages of development were observed in exposed snails and egg masses, respectively. Tested extracts of A. senegalensis, A. leiocarpus, C. retusa and V. amygdalina did not show appreciable toxicities to B. pfeifferi egg masses or adults. For demonstrating significant molluscicidal activity (LC 90 < 100 mg l -1 ) and toxicities to egg masses, we recommend further studies on the ethanolic extracts of D. sissoo fruits and roots.
“…of the cephalopedal mass (Evans et al 1986, Duncan and Sturrock 1987, Molgaard et al 2000, Al-Zanbagi et al 2001) and confirmed, if on microscopic examination, there were no observable heartbeats (Marston and Hostettmann 1985). The numbers of dead and live snails were recorded.…”
Section: Bioassay For Molluscicidal Activitymentioning
confidence: 96%
“…completely retracted into their shells (Evans et al 1986) they were discoloured (dos Santos et al 2000, Molgaard 2. et al 2000 or they were immobile and unresponsive to gentle prodding 3.…”
Section: Bioassay For Molluscicidal Activitymentioning
confidence: 98%
“…These include Phytolacca dodecandra, the best-studied plant molluscicide to date (Esser et al 2003), Millettia thonningii (Abbiw 1990) and Pavetta owariensis (Balde et al 1986, Evans et al 1986). …”
Laboratory studies were conducted to determine the potential molluscicidal activity of five Nigerian plant species used in alternative medicine practice as anthelmintics. Laboratory-bred adult Biomphalaria pfeifferi, the snail host of Schistosoma mansoni in Nigeria, and their viable 0-to 24-hour-old egg masses were separately exposed for 24 hours, in replicated bioassays, to 7.81-4 000 mg l -1 concentrations of crude ethanolic extracts of the leaves and roots of Annona senegalensis, Anogeissus leiocarpus, Crotalaria retusa, Dalbergia sissoo and Vernonia amygdalina. Fruits and stem bark of D. sissoo were also investigated. The 24-hour LC 50 and LC 90 values of extracts for target organisms were calculated using probit analysis. Susceptibility of target organisms to extracts varied with the plant species and morphological part. Egg masses and adult snails were most susceptible to D. sissoo fruit extract (LC 90 values of 89.29 and 74.33 mg l -1 , respectively) and least susceptible to V. amygdalina leaf extract (LC 90 values of 11 864. 09 and 6 241.61 mg l -1 , respectively). Concentration-dependent behavioural responses and embryo deaths at the gastrula/exogastrula and/or prehatch snail stages of development were observed in exposed snails and egg masses, respectively. Tested extracts of A. senegalensis, A. leiocarpus, C. retusa and V. amygdalina did not show appreciable toxicities to B. pfeifferi egg masses or adults. For demonstrating significant molluscicidal activity (LC 90 < 100 mg l -1 ) and toxicities to egg masses, we recommend further studies on the ethanolic extracts of D. sissoo fruits and roots.
“…The solvents used include water (natural, synthetic, distilled, dechlorinated and/or boiled; Archibald, 1933;Ritchie et al, 1963;Evans et al, 1986;Alard et al, 1991;Lwambo and Moyo, 1991;Monkiedje et al, 1991;Ferrer Lopez et al, 1993;Yong and Rodriguez, 1994;Brackenbury and Appleton, 1997a, b), butanol (Spielman and Lemma, 1973;Lemma and Yau, 1974a), petroleum ether and dichlorom ethane (El Hadi et al, 1984;Appleton et al, 1992), hexane (Pereira et al, 1978), methanol (Silva and Rouquayrol, 1971;Alard et al, 1991), ethanol Shabana et al, 1988) and aqueous carboxym ethyl cellulose (Alard et al, 1991), amongst others. Most of the extraction procedures have involved the specialized use of expensive chem icals and equipment.…”
Section: Preparation Of the Crude Aqueous Plant Extractmentioning
Several candidate plant molluscicides have been identified for possible incorporation into self-help control programmes against schistosomiasis, but their full potential has yet to be realised. This has been, in the most part, due to the absence of standardized assessment and toxicity protocols, and subsequently the inability to register crude plant products in their country of origin or internationally. In an attempt to solve this dilemma, a series of protocols has been designed which will provide a useful standardized guideline for investigations into plant molluscicides, as well as precipitate moves towards the establishment of internationally accepted guidelines for the assessment of various categories of plant biopesticides. Ultimately, being able to register a crude plant extract will permit health organizations such as the World Health Organization to promote the use of such material, especially in self-help control programmes, thereby improving the health standards of rural communities.
“…Bioactive compounds found in extracts of the mature seeds of the West African legume Millettia thonningii, have been shown to have molluscicidal activity toward adult Bulinus truncatus and Biomphalaria glabrata (Evans et al, 1986) and B. glabrata egg masses (Tang, 1994;Tang et al, 1995).…”
The study describes methods developed for using video-imaging technology to record and measure the velocity of Schistosoma mansoni miracidia. The efficacy of the classical bioassay procedure (a qualitative behavioural assay) was compared with that of the new quantitative protocol, for assessing the sublethal impact of a larvicidal dichloromethane extract of the seeds of Millettia thonningii on miracidia. The new technique confirmed the efficacy of the classical bioassay for rapid determination of the lethal and sub-lethal impact of larvicides but also provided quantitative information on sub-lethal impacts on miracidial velocity and shape.
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