Molekularbiologische Identifizierung von <i>Cunninghamella</i> spec.

Lemmer, Karin; Losert, Heidemarie; Rickerts, Volker; Just‐Nübling, G.; Sander, Anna; Kerkmann, Marie-Louise; Tintelnot, Kathrin

doi:10.1111/j.1439-0507.2002.tb04543.x

Cited by 15 publications

(3 citation statements)

References 14 publications

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“…The most common and practically the sole species of the genus Cunninghamella that is traditionally considered the etiologic agent of human infections is C. bertholletiae (21,24). However, Lemmer et al (15) reported in Germany a case of human infection by C. echinulata which was identified by sequencing the ITS region. In that study, the isolates of C. echinulata from environmental and clinical origins showed identical digestion patterns in the ITS restriction fragment length polymorphism by using TaqI and HinfI.…”

Section: Discussionmentioning

confidence: 99%

Spectrum of Zygomycete Species Identified in Clinically Significant Specimens in the United States

et al. 2009

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Several members of the order Mucorales (subphylum Mucoromycotina) are important agents of severe human infections. The identification of these fungi by using standard mycologic methods is often difficult and time consuming. Frequently, the etiological agent in clinical cases is reported either as a Mucor sp., which is not the most frequent genus of zygomycetes, or only as a member of the Mucorales. For this reason, the actual spectrum of species of zygomycetes and their incidences in the clinical setting is not well known. The goals of this study were to compare the results of the molecular identification of an important set of clinical isolates, received in a mycological reference center from different regions of the United States, with those obtained by using the traditional morphological methods and to determine the spectrum of species involved. We tested 190 isolates morphologically identified as zygomycetes by using sequencing of the internal transcribed spacer (ITS) region of the ribosomal DNA. Molecular identification revealed that Rhizopus oryzae represented approximately half (44.7%) of these isolates. The remainder was identified as Rhizopus microsporus (22.1%), Mucor circinelloides (9.5%), Mycocladus corymbifer (formerly Absidia corymbifera) (5.3%), Rhizomucor pusillus (3.7%), Cunninghamella bertholletiae (3.2%), Mucor indicus (2.6%), Cunninghamella echinulata (1%), and Apophysomyces elegans (0.5%). The most common anatomic sites for clinically significant zygomycetes, as determined by isolates sent to the Fungus Testing Laboratory for identification and/or susceptibility testing and included in this study, were the sinuses, lungs, and various cutaneous locations, at 25.8%, 26.8%, and 28%, respectively. These sites represented approximately 80% of the isolates evaluated. A high level of correlation (92.6%) between morphological and molecular identifications was found.

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Section: Discussionmentioning

confidence: 99%

Spectrum of Zygomycete Species Identified in Clinically Significant Specimens in the United States

et al. 2009

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“…The DNA was amplified with the primer pair ITS5/ITS4 21 . The PCR product was purified using the QIAquick ® Gel Extraction Kit (Qiagen) and directly sequenced with the primers ITS4 and ITS5 [10 pmol (10 μ l)] −1 22 . DNA sequencing was performed by the dideoxynucleotide termination method, using the dye deoxy terminator 3.1. chemistry and the ABI 377 automated sequencer (PE Applied Biosystems, Weiterstadt, Germany).…”

Section: Dna Sequencingmentioning

confidence: 99%

Follow‐up of epidemiological data of cryptococcosis in Austria, Germany and Switzerland with special focus on the characterization of clinical isolates

Tintelnot

Lemmer

Losert

et al. 2004

Mycoses

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The present survey in Austria, Germany and Switzerland continued the survey of cryptococcosis set up by the European Confederation of Medical Mycology (ECMM) in 1997. From 2000 to 2003 77 cases have been reported. An HIV infection is still the most important risk factor (68%). Young HIV+ women from ASIA contributed to the increase of cryptococcosis in females. A total of 129 clinical isolates of both surveys were genotyped by PCR fingerprinting to study the prevalence of different genotypes. The prevalence of Cryptococcus neoformans var. grubii (serotype A) with the genotypes VNA1 and VNA2 was higher in Germany and Austria (74.5%) than in Switzerland (52%), while in Switzerland the Cr. neoformans hybrids AD (26%) and Cr. neoformans var. neoformans (serotype D) (22%) were more prevalent compared with Germany and Austria (8 and 17.5% respectively). Cryptococcus gattii isolates were studied by FT-IR spectroscopy. DNA in the ITS region was sequenced to get further information about Cr. neoformans serotype AD strains and about the geographical origin of the Cr. gattii isolates. The ITS sequence of the serotype AD isolates of the genotypes VNAD1, VNAD2 and VNAD4 is usually identical to serotype A or serotype D respectively. In the three isolates of the genotype VNAD3 a genotype-specific sequence pattern was detected. Two autochthonous infections due to Cr. gattii could indicate that the genotype VGIV with the ITS type 'Asia 2' might be endemic in Europe.

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“…Based on our data and previous reports, 3,23 Cunninghamella blakesleeana and C. echinulata are no rare species in Iranian soil but have not yet been reported to cause infections. The number of reported cases caused by C. blakesleeana ( n = 1) and C. echinulata ( n = 3) is low in comparison to that of C. bertholletiae 52‐54 and might be suggestive of their lower virulence.…”

Section: Discussionmentioning

confidence: 88%

Frequency of occurrence, seasonal variation and antifungal susceptibility of opportunistic Mucorales isolated from hospital soils in Iran

Vaezi

Walther

Kurzai

et al. 2021

Mycoses

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Background Mucorales are opportunistic pathogens that can cause life‐threatening diseases predominantly in immunocompromised patients. Objectives This study aimed to investigate the frequency, seasonal variation and antifungal susceptibility of pathogenic Mucorales in the soil collected from seven hospitals in Urmia, Iran, between November 2017 and July 2018 in four different seasons. Methods Mucorales isolates obtained from soil were characterised based on conventional and molecular assays. In addition, in vitro antifungal susceptibility was performed using the CLSI M38Ed3 procedure. Results Out of 196 tested soil samples, 80 (40.8%) samples were positive for mucoralean fungi. Rhizopus arrhizus var. arrhizus (n = 47) was the most frequent species followed by Mucor circinelloides (n = 21) and Cunninghamella echinulata (n = 6). A seasonal variation in the frequency of Mucorales in soil was detected with a maximum of culture‐positive soil samples detected in wet autumn (43.2%) followed by winter (23.4%), summer (19.7%) and spring (13.6%). In vitro antifungal susceptibility testing for 80 environmental isolates exhibited MIC of ≤2 μg/ml for amphotericin B indicating the smallest range of MIC variation among the tested Mucorales (range: 0.125–2 μg/ml). Among the azoles, posaconazole was the most effective antifungals (GM MIC, 0.724 μg/ml). Conclusions We considered associations of species and seasonal frequencies between soil mucoralean fungi and mucormycosis. The effect of opportunistic Mucorales dominating in the soil and prevalent causative agents of mucormycosis in Iran reported in the literatures but more comprehensive studies are needed to confirm this conclusion.

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Molekularbiologische Identifizierung von Cunninghamella spec.

Cited by 15 publications

References 14 publications

Spectrum of Zygomycete Species Identified in Clinically Significant Specimens in the United States

Spectrum of Zygomycete Species Identified in Clinically Significant Specimens in the United States

Follow‐up of epidemiological data of cryptococcosis in Austria, Germany and Switzerland with special focus on the characterization of clinical isolates

Frequency of occurrence, seasonal variation and antifungal susceptibility of opportunistic Mucorales isolated from hospital soils in Iran

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