Molecules in mammalian brain that interact with the colchicine site on tubulin.

Lockwood, Arthur H.

doi:10.1073/pnas.76.3.1184

Cited by 43 publications

(23 citation statements)

References 21 publications

(17 reference statements)

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“…To induce accumulation of OFQ/N in the neuronal soma, 4 hours after EB treatment all animals received a lateral ventricular infusion of 50 μg of colchicine dissolved in 1.0 μl of 0.9% saline containing 10% dimethyl sulfoxide [33]. The infusions were performed using an infusion pump (Stoelting Co., Wood Dale, IL) at a rate of 0.5 μl/min for a total volume of 1.0 μl [27].…”

Section: Methodsmentioning

confidence: 99%

Estradiol Upregulates Progesterone Receptor and Orphanin FQ Colocalization in Arcuate Nucleus Neurons and Opioid Receptor-Like Receptor-1 Expression in Proopiomelanocortin Neurons That Project to the Medial Preoptic Nucleus in the Female Rat

Sanathara

Moreas²,

Mahavongtrakul³

et al. 2014

Neuroendocrinology

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Background: Ovarian steroids regulate sexual receptivity in the female rat by acting on neurons that converge on proopiomelanocortin (POMC) neurons in the arcuate nucleus of the hypothalamus (ARH) that project to the medial preoptic nucleus (MPN). Estradiol rapidly activates these neurons to release β-endorphin that activates MPN μ-opioid receptors (MOP) to inhibit lordosis. Lordosis is facilitated by the subsequent action of progesterone that deactivates the estradiol-induced MPN MOP activation. Orphanin FQ (OFQ/N; also known as nociceptin) infusions into the ARH, like progesterone, deactivate MPN MOP and facilitate lordosis in estradiol-primed rats. OFQ/N reduces the activity of ARH β-endorphin neurons through post- and presynaptic mechanisms via its cognate receptor, ORL-1. Methods: We tested the hypotheses that progesterone receptors (PR) are expressed in ARH OFQ/N neurons by immunohistochemistry and ORL-1 is expressed in POMC neurons that project to the MPN by combining Fluoro-Gold injection into the MPN and double-label fluorescent in situ hybridization (FISH). We also hypothesized that estradiol increases coexpression of PR-OFQ/N and ORL-1-POMC in ARH neurons of ovariectomized rats. Results: The number of PR- and OFQ/N-immunopositive ARH neurons was increased as was their colocalization by estradiol treatment. FISH for ORL-1 and POMC mRNA revealed a subpopulation of ARH neurons that was triple labeled, indicating these neurons project to the MPN and coexpress ORL-1 and POMC mRNA. Estradiol was shown to upregulate ORL-1 and POMC expression in MPN-projecting ARH neurons. Conclusion: Estradiol upregulates the ARH OFQ/N-ORL-1 system projecting to the MPN that regulates lordosis.

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Section: Methodsmentioning

confidence: 99%

Estradiol Upregulates Progesterone Receptor and Orphanin FQ Colocalization in Arcuate Nucleus Neurons and Opioid Receptor-Like Receptor-1 Expression in Proopiomelanocortin Neurons That Project to the Medial Preoptic Nucleus in the Female Rat

Sanathara

Moreas²,

Mahavongtrakul³

et al. 2014

Neuroendocrinology

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“…Faivre et al [1985] demon strated that during the early stages of post natal development microtubules in the den drites of Purkinje cells are less stable to cold depolymerization than those in adult ani mals. It is interesting to note that a number of endogenous colchicine-like substances have been isolated which interact with tubu lin in a manner similar to that of colchicine [Lockwood, 1979;Sherline et al, 1979;Nishida and Sakai, 1980]. Thus, it may be likely that during the normal development of neurons colchicine-like factors may be in duced, leading to a destabilization of micro tubules and the resultant selective transport of lysosomes to the dendrites.…”

Section: Discussionmentioning

confidence: 99%

Examination of the Transient Distribution of Lysosomes in Neurons of Developing Rat Brains

Roberts

Gorenstein²

1987

Dev Neurosci

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We have previously observed that lysosomes redistribute from their normal location in neuronal cell bodies to the dendrites following an intracerebroventricular injection of an antimitotic such as colchicine, vinblastine or vincristine. In the present study, we have followed the developmental distribution of lysosomes in the brains of untreated rats, using a lysosomal marker enzyme, dipeptidylaminopeptidase II. A relatively high concentration of neuronal lysosomes was found in the dendrites of olfactory bulb mitral cell neurons and in hippocampal granule and pyramidal cell neurons from postnatal day 1 (PI) to P8. As the animals matured, the pattern of lysosomal enzyme distribution was reversed. Lysosomes became progressively less concentrated in the dendrites and more concentrated in neuronal cell bodies. In cerebellar Purkinje cells, lysosomes were only found in the cell bodies during the first week after birth. Between P9 and P19, lysosomes appeared in the dendrites of these neurons and, with maturity, progressively disappeared from the dendrites and were concentrated mainly in cell bodies. The presence of lysosomes in the dendrites of developing animals suggests that the transport of lysosomes to the dendrites, induced by microtubule poisons, mimics a physiological process which is normally present during development.

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“…7). We propose that in untreated Kc cells, a significant proportion of unpolymerized tubulin is associated with a factor, perhaps a protein, with properties similar to those described by Lockwood [1979]. This association occurs at or near the colchicinebinding site, or at a remote site that leads to an altered conformation at the binding site.…”

Section: Discussionmentioning

confidence: 83%

Tubulin content and synthesis in differentiating drosophila cells in culture

1980

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Drosophila Kc cells exposed to physiological doses of the moulting hormone, beta-ecdysone, elongate, become motile, and subsequently aggregate. This pattern of morphogenesis was found to require the assembly of a microtubular cytoskeleton. Tubulin content was significantly increased in hormone-treated cells when compared to controls, as measured by a 3H-colchicine-binding assay. However, determinations of rates of tubulin synthesis and breakdown revealed no difference between control and hormone-treated cells for either parameter. When tubulin content was assayed by methods that do not depend on colchicine-binding activity, no difference between hormone-treated and control cells was observed. These results are discussed in terms of a model in which beta-ecdysone affects the distribution of tubulin in "assembly-active" and "assembly-inactive" pools.

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Molecules in mammalian brain that interact with the colchicine site on tubulin.

Cited by 43 publications

References 21 publications

Estradiol Upregulates Progesterone Receptor and Orphanin FQ Colocalization in Arcuate Nucleus Neurons and Opioid Receptor-Like Receptor-1 Expression in Proopiomelanocortin Neurons That Project to the Medial Preoptic Nucleus in the Female Rat

Estradiol Upregulates Progesterone Receptor and Orphanin FQ Colocalization in Arcuate Nucleus Neurons and Opioid Receptor-Like Receptor-1 Expression in Proopiomelanocortin Neurons That Project to the Medial Preoptic Nucleus in the Female Rat

Examination of the Transient Distribution of Lysosomes in Neurons of Developing Rat Brains

Tubulin content and synthesis in differentiating drosophila cells in culture

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