Molecules and Regulation in Lymphocyte Migration

Hamann, Alf; Thiele, H G

doi:10.1111/j.1600-065x.1989.tb00011.x

Cited by 44 publications

(28 citation statements)

References 59 publications

(48 reference statements)

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“…We therefore studied the effect of the bispecific Ab on tumor cell migration. Our data for homing of 38C-13 cells are in agreement with previously published data on 38C-13 and resemble the homing pattern of other lymphoid tumors (25)(26)(27)(28). The Ab inhibition studies demonstrate that, unlike anti-Id Abs, the anti-Id ϫ anti-LFA-1 bispecific Abs inhibited migration of 38C-13 cells in a way that correlated with their inhibitory effect on metastasis.…”

Section: Discussionsupporting

confidence: 82%

“…Thus, by 24 h the cells had left the lungs, and their majority accumulated in the liver and spleen. This pattern of homing has been previously reported for tumor cells, including 38C-13C cells, as well as for activated T cells (25)(26)(27)(28). Migration of 38C-13 cells to liver and lymph nodes was inhibited by the bispecific Ab, while migration to spleen was not affected (Fig.…”

Section: Anti-id ϫ Anti-lfa-1 Bispecific Abs Inhibit Migration Of 38cmentioning

confidence: 62%

“…LFA-1 plays an important accessory role in lymphocyte homing to lymph nodes, which is partially inhibited by anti-LFA-1 Abs. In contrast, LFA-1 does not play an important role in lymphocyte migration to the spleen (25,39,40). Similarly, it has been suggested that interactions between tumor cells and endothelium at the site of extravasation represent one mechanism that determines organ-specific metastasis.…”

Section: Discussionmentioning

confidence: 98%

“…Similarly, it has been suggested that interactions between tumor cells and endothelium at the site of extravasation represent one mechanism that determines organ-specific metastasis. Although the homing behavior of lymphoma cells closely resembles that of activated lymphocytes (25), the role of LFA-1 in migration of the two cell types may differ. Hence, while lymphocytes and lymphoma cells similarly require LFA-1 for lymph node homing, but not for spleen homing, they differ in the mechanisms regulating migration to the liver.…”

Section: Discussionmentioning

confidence: 99%

“…This accumulation was significantly inhibited by the bispecific Ab, but not by the anti-Id Ab. Hamann and Thiele (25) reported that anti-LFA-1 Abs did not inhibit homing of YAC-1 cells to the liver. This discrepancy may be accounted for by the difference in the cell types, YAC-1 being a T cell tumor and 38C-13 being a B cell tumor.…”

Section: Discussionmentioning

confidence: 99%

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Anti-idiotype × Anti-LFA-1 Bispecific Antibodies Inhibit Metastasis of B Cell Lymphoma

Cohen

Haimovich

Hollander

2003

The Journal of Immunology

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Abs to adhesion molecules can block tumor metastasis. However, they may also block the function of normal cells. To circumvent this adverse effect, we proposed the use of bispecific Abs that bind simultaneously to an adhesion receptor and to a tumor-specific Ag. Such Abs bind more avidly to tumor cells that coexpress both target Ags than to normal cells. The Id of the surface Ig of malignant B lymphocytes is a tumor-specific Ag. We therefore produced a bispecific Ab with specificity to the adhesion molecule LFA-1 and to the Id of the murine B cell lymphoma 38C-13. Here we demonstrate that this Ab blocked liver metastasis in mice carrying primary s.c. tumors and partially inhibited lymph node metastasis. Migration of 38C-13 cells to liver and lymph nodes was inhibited by the bispecific Ab, while migration to spleen was not affected. Hence, the bispecific Ab-mediated reduction in liver and lymph node metastasis resulted at least in part from reduced homing to these organs. In contrast to anti-LFA-1 monospecific Abs, the anti-Id × anti-LFA-1 bispecific Ab did not affect immune responses such as delayed-type hypersensitivity. Hence, bispecific Abs against adhesion molecules and against tumor-specific Ags may selectively block tumor metastasis in a way that may leave much of the immune system intact.

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Section: Discussionsupporting

confidence: 82%

Section: Anti-id ϫ Anti-lfa-1 Bispecific Abs Inhibit Migration Of 38cmentioning

confidence: 62%