Molecularly Imprinted TiO₂-Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry for Selectively Detecting α-Cyclodextrin

Abstract

This study describes a new means to conduct molecular recognition-based analysis using mass spectrometry. Taking advantage of the unique characteristic of the absorption capacity of the TiO(2) sol-gel material in the UV region, a TiO(2) sol-gel-deposited thin film was employed as the sample substrate to assist in UV laser desorption/ionization of analytes. Sol-gels are polymeric materials that are easy to prepare and modify at low temperatures. Molecularly imprinted TiO(2) sol-gels were generated for molecular recognition-based analysis. alpha-Cyclodextrin (CD) was selected as the template molecule and doped into TiO(2) in a sol-gel reaction. The molecularly imprinted TiO(2) sol was spin-coated on a glass slide, and appropriate template cavities in the TiO(2) sol-gel material were formed after the template molecules were removed. We demonstrate that this modified glass slide can be used to select alpha-CD from a sample solution containing equal amounts of alpha-, beta-, and gamma-CD (50 ppb each, 18 mL); alpha-CD was directly detected from the modified glass slide by matrix-assisted laser desorption/ionization mass spectrometry without the addition of extra matrix. This approach provides a new detection method for molecular recognition-based analysis.

A novel laser desorption/ionization method using through hole porous alumina membranes

“…To date a number of ionization techniques that belong to SALDI have been proposed,1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, ...…”

“…nanoparticles as typical, in treatable forms such as suspension liquids in order to ensure that the photon energies of the laser can act to desorp and ionize analytes 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45. Indeed, the soft ionization laser method such as the original form of MALDI also belongs to this category 1.…”

Characterization of synthesized titanium oxide nanoclusters by MALDI-TOF mass spectrometry

“…Matrix solutions were prepared by dissolving 10 mg of dithranol in 1 mL of dry chloroform without the use of methanol. Naturally occurring titanium is composed of five stable isotopes: 46 Ti (10.8%), 47 Ti (9.9%), 49 Ti (7.5%), 50 Ti (7.3%), with 48 Ti (73.8%) being the most abundant; this unique isotopic pattern facilitates the assignment of titanium-containing species. Using LDI without any matrix also gave signals corresponding to titanium oxide cations.…”

Nanomaterial-Assisted Laser Desorption Ionization for Mass Spectrometry-Based Biomedical Analysis

“…Another interesting material for NALDI is nanotitania [25][26][27][28]. TiO 2 semiconducting nanoparticles are widely used in the fi eld of phofield of phoof photocatalysis [29] and for the design of dye-sensitized solar cells.…”

“…The breakthrough for large molecule LDI Carbon 1995 Graphite-assisted LDI [33] Porous silicon 1999 Most widely used matrix-free LDI method [5] Gold clusters 2004 Developed from secondary ion mass spectrometry [16] Titania 2004 Titania sol-gel-assisted LDI [27] LDI: Laser desorption ionization.…”