Molecularly Imprinted Polypyrrole for DNA Determination

Abstract

In this study graphite electrodes modified by a thin DNA‐imprinted polypyrrole layer, which was able to bind specific target‐DNA, are reported. For this aim, electrochemical synthesis of polypyrrole was performed on a pencil graphite electrode by cyclic voltammetry (CV) or by potential pulse sequences (PPS). The modified electrode surface was used for electrochemical determination of target‐DNA by differential pulse voltammetry. According to our best knowledge this is a first report on the application of DNA‐imprinted polymer for the determination of target‐DNA. The results showed that the molecularly imprinted polypyrrole (MIPPy) layer that formed on the carbon electrode surface was sensitive for target‐DNA, while the nonimprinted polypyrrole layer was not sensitive to the same target‐DNA. Comparison of electrodes modified using PPS and CV techniques is presented.

Evaluation of theophylline imprinted polypyrrole film

“…Nominating the ratio N/N s as F (fill factor) the following solution of this equation is obtained: Fig. 5 shows that the kinetics of theophylline interaction when the PBS influence to the capacity change is negligible could be described by formula (4). At equilibrium condition (when dN/ dt = 0) the N eq value can be obtained using following formula:…”

“…MIPs have high mechanical and chemical stability, are easy to prepare and are inexpensive. The MIPs behave as receptors that specifically interact with corresponding ligands [3] and for this reason they can be used in the design of chemical sensors, which are acting similarly to affinity biosensors, which usually are based on immobilized antibodies, receptors or single-stranded DNA [4].…”

“…Several procedures are used in order to deposit MIP films on a signal transducer surface [5], including (1) electropolymerization [4], (2) drop-coating, (3) formation of membranes with entrapped conducting materials such as carbon nanotubes, graphite, or carbon black, (4) in situ chemical polymerization. Each of the mentioned polymerization methods has its advantages, but among many Ppy formation methods, electropolymerization for the preparation of sensors is preferable method resulting in the formation of MIP-based films.…”

Molecular imprinted photonic crystal for sensing of biomolecules

“…Molecular imprinting has proven to be particularly successful for small molecules. Recent years, much attention has been paid to the imprinting of biomolecules such as proteins [54][55], DNA [56], and even whole cells [57] and viruses [58][59] for its potential applications in biomaterials separation, purification, biosensor, and mimicking enzyme and antibody [60]. Rossetti et al synthesized molecularly imprinted polymers (MIPs) targeting the proteotypic peptide of ProGRP by surface-initiated reversible addition-fragmentation chain transfer polymerization.…”

Electrochemical evaluation of troponin T imprinted polymer receptor

“…Although, there are many reports of MIPs for small templates however, substantially less articles are published on MIPs for large templates, e.g. proteins (Kan et al, 2012;Ramanaviciene and Ramanavicius, 2004;Ratautaite et al, 2013). Furthermore, removal of proteins from imprinted polymers is rather difficult due to their high molecular weights, which retard diffusion through the dense polymer network.…”