Molecularly imprinted polymers for the recognition of biomarkers of certain neurodegenerative diseases

Pilvenyte, Greta; Ratautaitė, Vilma; Boguzaite, Raimonda; Samukaitė-Bubnienė, Urtė; Plaušinaitis, Deivis; Ramanavičienė, Almira; Bechelany, Mikhael; Ramanavičius, Arūnas

doi:10.1016/j.jpba.2023.115343

Cited by 18 publications

(4 citation statements)

References 82 publications

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“…Neurological disorders are malfunctions affecting the nervous system due to structural, biochemical, or electrical abnormalities in the brain, spinal cord, or other nerves of the human body [109]. Hence, to facilitate the achievement of curative or management goals of medical research via the provision of clinical evaluation and diagnosis, there is a need to design biomimetic sensors to detect known biomarkers of these disorders [110]. Neurotransmitters are responsible for the transmission of signals between neurons and non-neuronal body cells across chemical synapses.…”

Section: Neurological Disordersmentioning

confidence: 99%

Electrochemically Synthesized MIP Sensors: Applications in Healthcare Diagnostics

Ayankojo,

Reut,

Syritski

2024

Biosensors

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Early-stage detection and diagnosis of diseases is essential to the prompt commencement of treatment regimens, curbing the spread of the disease, and improving human health. Thus, the accurate detection of disease biomarkers through the development of robust, sensitive, and selective diagnostic tools has remained cutting-edge scientific research for decades. Due to their merits of being selective, stable, simple, and having a low preparation cost, molecularly imprinted polymers (MIPs) are increasingly becoming artificial substitutes for natural receptors in the design of state-of-the-art sensing devices. While there are different MIP preparation approaches, electrochemical synthesis presents a unique and outstanding method for chemical sensing applications, allowing the direct formation of the polymer on the transducer as well as simplicity in tuning the film properties, thus accelerating the trend in the design of commercial MIP-based sensors. This review evaluates recent achievements in the applications of electrosynthesized MIP sensors for clinical analysis of disease biomarkers, identifying major trends and highlighting interesting perspectives on the realization of commercial MIP-endowed testing devices for rapid determination of prevailing diseases.

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Section: Neurological Disordersmentioning

confidence: 99%

Electrochemically Synthesized MIP Sensors: Applications in Healthcare Diagnostics

Ayankojo,

Reut,

Syritski

2024

Biosensors

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“…The synthetic preparation of the polymer involves a multiple-stage process using the molecular imprinted polymer (MIP) method, a technique refined through specific steps including selection of target molecule and functional monomers, addition of initiators and cross-linkers, preparation of the molecular printing template, polymerization, template removal, cleaning, and characterization of the obtained polymer, and employing the obtained MIP for the recognition and extraction of the target molecule. Effective extraction techniques play a pivotal role in detecting biological analytes by facilitating the isolation and concentration of specific molecules from complex biological samples, thereby improving the precision and sensitivity of analytical methods. − Applications include chemical separation, drug delivery, or biosensors. ,− …”

Section: Introductionmentioning

confidence: 99%

Selective Extraction and Quantification of Hemoglobin Based on a Novel Molecularly Imprinted Nanopolymeric Structure of Poly(acrylamide-vinyl imidazole)

Şarkaya,

Özçelik,

Yaşar

et al. 2024

ACS Omega

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Imbalances in hemoglobin (Hb) levels can lead to conditions such as anemia or polycythemia, emphasizing the importance of precise Hb extraction from blood. To address this, a novel synthetic imprinted polymer was meticulously developed for capturing and separating Hb. Poly(acrylamide-vinylimidazole) nanopolymer (poly(AAm-VIM)) was synthesized using acrylamide and vinyl imidazole as functional monomers through surfactant-free emulsion polymerization. Characterization using FTIR, particle size, zeta potential, and SEM ensured the polymer's structure. The Hb-imprinted nanopolymer (Hbpoly(AAm-VIM)) demonstrated notable specificity, with a calculated Hb-specific adsorption value (Q max ) of 3.7377 mg/g in a medium containing 2.5 mg/mL Hb. The molecularly imprinted polymer (MIP) exhibited approximately 5 times higher Hb adsorption than the nonimprinted polymer (NIP). Under the same conditions, the imprinted nanopolymer displayed 2.39 and 2.17 times greater selectivity for Hb over competing proteins such as bovine serum albumin (BSA) and lysozyme (Lys), respectively. Also, SDS-PAGE analysis results confirmed the purification of Hb by the molecularly imprinted nanopolymer. These results underscore the heightened specificity and efficacy of the molecularly imprinted nanopolymer in selectively targeting Hb atoms among other proteins. Incorporating such polymers is justified by their notable affinity, cost-effectiveness, and facile production. This research contributes valuable insights into optimizing synthetic imprinted polymers for efficient Hb extraction, with potential in medical diagnostics and treatment applications.

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“…Regarding recognition elements, several molecules have been proposed, such as peptides [21], aptamers [22,23], or molecular-imprinted polymers (MIPs) [24]. The most exploited class, however, is that of antibodies [22,25] or antibody fragments [19], thanks to their sensitivity and selectivity properties; however, aptamers are also emerging as convenient recognition elements.…”

Section: Introductionmentioning

confidence: 99%

Optimization of Surface Functionalizations for Ring Resonator-Based Biosensors

Ardoino,

Lunelli,

Pucker

et al. 2024

Sensors

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Liquid biopsy is expected to become widespread in the coming years thanks to point of care devices, which can include label-free biosensors. The surface functionalization of biosensors is a crucial aspect that influences their overall performance, resulting in the accurate, sensitive, and specific detection of target molecules. Here, the surface of a microring resonator (MRR)-based biosensor was functionalized for the detection of protein biomarkers. Among the several existing functionalization methods, a strategy based on aptamers and mercaptosilanes was selected as the most highly performing approach. All steps of the functionalization protocol were carefully characterized and optimized to obtain a suitable protocol to be transferred to the final biosensor. The functionalization protocol comprised a preliminary plasma treatment aimed at cleaning and activating the surface for the subsequent silanization step. Different plasma treatments as well as different silanes were tested in order to covalently bind aptamers specific to different biomarker targets, i.e., C-reactive protein, SARS-CoV-2 spike protein, and thrombin. Argon plasma and 1% v/v mercaptosilane were found as the most suitable for obtaining a homogeneous layer apt to aptamer conjugation. The aptamer concentration and time for immobilization were optimized, resulting in 1 µM and 3 h, respectively. A final passivation step based on mercaptohexanol was also implemented. The functionalization protocol was then evaluated for the detection of thrombin with a photonic biosensor based on microring resonators. The preliminary results identified the successful recognition of the correct target as well as some limitations of the developed protocol in real measurement conditions.

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Molecularly imprinted polymers for the recognition of biomarkers of certain neurodegenerative diseases

Cited by 18 publications

References 82 publications

Electrochemically Synthesized MIP Sensors: Applications in Healthcare Diagnostics

Electrochemically Synthesized MIP Sensors: Applications in Healthcare Diagnostics

Selective Extraction and Quantification of Hemoglobin Based on a Novel Molecularly Imprinted Nanopolymeric Structure of Poly(acrylamide-vinyl imidazole)

Optimization of Surface Functionalizations for Ring Resonator-Based Biosensors

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