Molecularly imprinted polymers for capturing and sensing proteins: Current progress and future implications

Ansari, Saeedeh; Masoum, Saeed

doi:10.1016/j.trac.2019.02.008

Cited by 120 publications

(43 citation statements)

References 94 publications

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“…Furthermore, precipitation polymerization in which highly diluted monomer solutions are employed can negatively affect the template–monomer interaction and thus sensitivity and selectivity. Even emulsion imprinting with a potential platform disruption due to the stabilizers/surfactants addition and remaining residual of these additives even after extensive washing steps possess limitations when compared to MIP grafted onto MNPs [ 62 , 106 ]. In these molecular imprinting methods, a high level of crosslinking is used to ensure template binding specificity, and thus the resulting rigid polymeric network hinders the penetration and accessibility of the solvents to the template embedded in this polymer matrix.…”

Section: Magnetic Molecularly Imprinted Polymer (Mmip) Preparationmentioning

confidence: 99%

Developments of Smart Drug-Delivery Systems Based on Magnetic Molecularly Imprinted Polymers for Targeted Cancer Therapy: A Short Review

Sanadgol

Wackerlig

2020

Pharmaceutics

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Cancer therapy is still a huge challenge, as especially chemotherapy shows several drawbacks like low specificity to tumor cells, rapid elimination of drugs, high toxicity and lack of aqueous solubility. The combination of molecular imprinting technology with magnetic nanoparticles provides a new class of smart hybrids, i.e., magnetic molecularly imprinted polymers (MMIPs) to overcome limitations in current cancer therapy. The application of these complexes is gaining more interest in therapy, due to their favorable properties, namely, the ability to be guided and to generate slight hyperthermia with an appropriate external magnetic field, alongside the high selectivity and loading capacity of imprinted polymers toward a template molecule. In cancer therapy, using the MMIPs as smart-drug-delivery robots can be a promising alternative to conventional direct administered chemotherapy, aiming to enhance drug accumulation/penetration into the tumors while fewer side effects on the other organs. Overview: In this review, we state the necessity of further studies to translate the anticancer drug-delivery systems into clinical applications with high efficiency. This work relates to the latest state of MMIPs as smart-drug-delivery systems aiming to be used in chemotherapy. The application of computational modeling toward selecting the optimum imprinting interaction partners is stated. The preparation methods employed in these works are summarized and their attainment in drug-loading capacity, release behavior and cytotoxicity toward cancer cells in the manner of in vitro and in vivo studies are stated. As an essential issue toward the development of a body-friendly system, the biocompatibility and toxicity of the developed drug-delivery systems are discussed. We conclude with the promising perspectives in this emerging field. Areas covered: Last ten years of publications (till June 2020) in magnetic molecularly imprinted polymeric nanoparticles for application as smart-drug-delivery systems in chemotherapy.

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Section: Magnetic Molecularly Imprinted Polymer (Mmip) Preparationmentioning

confidence: 99%

Developments of Smart Drug-Delivery Systems Based on Magnetic Molecularly Imprinted Polymers for Targeted Cancer Therapy: A Short Review

Sanadgol

Wackerlig

2020

Pharmaceutics

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“…MIPs are increasingly investigated due to their numerous advantages: high affinity towards analytes of interest; chemical, physical, and thermal stability; and straightforward preparation [ 4 ]. The rational design of molecular imprinting consists in generating synthetic receptors mimicking natural recognition elements [ 5 ]. Concretely a polymeric matrix is first achieved around the molecules of interest.…”

Section: Introductionmentioning

confidence: 99%

Contribution to the Understanding of the Interaction between a Polydopamine Molecular Imprint and a Protein Model: Ionic Strength and pH Effect Investigation

Tlili

Attia

Khaoulani

et al. 2021

Sensors

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Several studies were devoted to the design of molecularly imprinted polymer (MIP)-based sensors for the detection of a given protein. Here, we bring elements that could contribute to the understanding of the interaction mechanism involved in the recognition of a protein by an imprint. For this purpose, a polydopamine (PDA)-MIP was designed for bovine serum albumin (BSA) recognition. Prior to BSA grafting, the gold surfaces were functionalized with mixed self-assembled monolayers of (MUDA)/(MHOH) (1/9, v/v). The MIP was then elaborated by dopamine electropolymerization and further extraction of BSA templates by incubating the electrode in proteinase K solution. Three complementary techniques, electrochemistry, zetametry, and Fourier-transform infrared spectrometry, were used to investigate pH and ionic strength effects on a MIP’s design and the further recognition process of the analytes by the imprints. Several MIPs were thus designed in acidic, neutral, and basic media and at various ionic strength values. Results indicate that the most appropriate conditions, to achieve a successful MIPs, were an ionic strength of 167 mM and a pH of 7.4. Sensitivity and dissociation constant of the designed sensor were of order of (3.36 ± 0.13) µA·cm−2·mg−1·mL and (8.56 ± 6.09) × 10−11 mg/mL, respectively.

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“…However, difficulties can occur when using biomolecules as templates (proteins being particularly problematic) due to their high sensitivity to many solvents which are often employed in the imprinting process, resulting in the denaturing of the template molecule. Traditionally, MIPs have been synthesised successfully for smaller molecules (<1.5 kDa), however, complications often arise regarding macromolecule templates and the subsequent ability to effectively bind to the analyte [19,20]. This aspect has historically rendered MIPs as a less appealing alternative for consideration in cardiac disease detection-based POC platforms since cardiac biomarkers are susceptible to degradation in suboptimal environments (the majority of cardiac biomarkers also being larger than 1.5 kDa) [21,22].…”

Section: Introductionmentioning

confidence: 99%

“…This aspect has historically rendered MIPs as a less appealing alternative for consideration in cardiac disease detection-based POC platforms since cardiac biomarkers are susceptible to degradation in suboptimal environments (the majority of cardiac biomarkers also being larger than 1.5 kDa) [21,22]. Moreover, the efficacy of a MIP is greatly influenced by the specific technique employed during synthesis, with a variety of methods reported, many of whom are extensively reviewed throughout the literature [19,23,24,25]. Some notable applications of MIPs include trace analyte extraction, chromatographic separation and use in various chemical sensors, although, they have yet to find widespread commercial use as a solid phase [26,27,28,29,30,31].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Molecularly Imprinted Polymers for Point-of-Care Testing for Cardiovascular Disease

Regan

Walsh

O’Kennedy

et al. 2019

Sensors

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Molecular imprinting is a rapidly growing area of interest involving the synthesis of artificial recognition elements that enable the separation of analyte from a sample matrix and its determination. Traditionally, this approach can be successfully applied to small analyte (<1.5 kDa) separation/ extraction, but, more recently it is finding utility in biomimetic sensors. These sensors consist of a recognition element and a transducer similar to their biosensor counterparts, however, the fundamental distinction is that biomimetic sensors employ an artificial recognition element. Molecularly imprinted polymers (MIPs) employed as the recognition elements in biomimetic sensors contain binding sites complementary in shape and functionality to their target analyte. Despite the growing interest in molecularly imprinting techniques, the commercial adoption of this technology is yet to be widely realised for blood sample analysis. This review aims to assess the applicability of this technology for the point-of-care testing (POCT) of cardiovascular disease-related biomarkers. More specifically, molecular imprinting is critically evaluated with respect to the detection of cardiac biomarkers indicative of acute coronary syndrome (ACS), such as the cardiac troponins (cTns). The challenges associated with the synthesis of MIPs for protein detection are outlined, in addition to enhancement techniques that ultimately improve the analytical performance of biomimetic sensors. The mechanism of detection employed to convert the analyte concentration into a measurable signal in biomimetic sensors will be discussed. Furthermore, the analytical performance of these sensors will be compared with biosensors and their potential implementation within clinical settings will be considered. In addition, the most suitable application of these sensors for cardiovascular assessment will be presented.

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Molecularly imprinted polymers for capturing and sensing proteins: Current progress and future implications

Cited by 120 publications

References 94 publications

Developments of Smart Drug-Delivery Systems Based on Magnetic Molecularly Imprinted Polymers for Targeted Cancer Therapy: A Short Review

Developments of Smart Drug-Delivery Systems Based on Magnetic Molecularly Imprinted Polymers for Targeted Cancer Therapy: A Short Review

Contribution to the Understanding of the Interaction between a Polydopamine Molecular Imprint and a Protein Model: Ionic Strength and pH Effect Investigation

Evaluation of Molecularly Imprinted Polymers for Point-of-Care Testing for Cardiovascular Disease

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