Molecularly imprinted polymers based on SBA-15 for selective solid-phase extraction of baicalein from plasma samples

Abstract

Highly selective molecularly imprinted mesoporous silica polymer (SBA-15@MIP) for baicalein (BAI) extraction was synthesized using a surface molecular imprinting technique on the SBA-15 supporter. Computational simulation was used to predict the optimal functional monomer for the rational design of SBA-15@MIP. Meanwhile, high adsorption capacity was obtained when a suitable yield of molecularly imprinted polymers (MIPs) layer was grafted onto the surface of SBA-15. Characterization and performance tests of the obtained polymer revealed that SBA-15@MIP possessed a highly ordered mesoporous structure, reached saturated adsorption within 60 min, and exhibited higher sorption capacity to the target molecule BAI compared with non-imprinted mesoporous silica polymer (SBA-15@NIP) and SBA-15. Finally, SBA-15@MIP was successfully applied to solid-phase extraction (SPE) coupled with high-performance liquid chromatography and ultraviolet detection (HPLC-UV) for the determination of trace BAI in plasma samples. Mean recoveries of BAI through the molecularly imprinted solid-phase extraction (MISPE) sorbent, non-imprinted solid-phase extraction (NISPE) sorbent, and SBA-15 solid-phase extraction (SBA-15-SPE) sorbent were 94.4, 22.7, and 10.7 %, respectively, and the relative standard deviations were 2.9, 2.6, and 3.6 %, respectively. These results reveal that SBA-15@MIP as a SPE sorbent has good applicability to selectively separate and enrich trace BAI from complex samples.

The Multi-Template Molecularly Imprinted Polymer Based on SBA-15 for Selective Separation and Determination of Panax notoginseng Saponins Simultaneously in Biological Samples

“…The surface of the as-prepared SBA-15 was modified by MPS according to our previous report [31]. Briefly, 500.0 mg SBA-15, 10 mL MPS and 50 mL anhydrous toluene were placed into a 100 mL three-necked flask and stirred.…”

“…In our initial studies, we synthesized highly selective single-template MIPs based on mesoporous materials for selective separation and quantitative analysis of natural products in biological specimens [31,32]. As we all know, the single-template MIPs have recognition sites only for one target molecule, which leads to poor selectivity and affinity for a family of analytes in most cases [33,34], except that experimental conditions were optimized, such as the optimization of extraction process [35].…”

Preparation of Diamine-modified Ordered Mesoporous SBA-15 and Its Adsorption Property to Pb2+

“….9°,confirmsthatmodifiedmaterialstillhasthetypicalorderedsix-partyporestructure [15][16][17][18] ,(110) and(200)characteristicpeakintensitygraduallydeclinewiththeincreaseofdiaminethematerial contain.Whenthecontentofgrafteddiamineupto20%(110)and(200)characteristicpeakalmost disappeared,thatmeansgraftingreactivetoomuchwillaffecttheorderofmesoporousmaterials. ,belongstothestretchingvibrationpeakofSi-OH.Atabout775.69cm -1 belongsto Si-O-Sisymmetricstretchingvibrationpeak,and1120.87cm -1 ,asymmetricstretchingvibration peak [19] .And,1632.…”

Rapid Detection of Kaempferol Using Surface Molecularly Imprinted Mesoporous Molecular Sieves Embedded with Carbon Dots

“…. SBA-15-MPS was fabricated using method reported by He et al [21]. For this purpose, 500 mg of SBA-15 was mixed with 50 mL of toluene.…”