Molecularly Imprinted Polymer-Based Sensors for Protein Detection

Abstract

The accurate detection of biological substances such as proteins has always been a hot topic in scientific research. Biomimetic sensors seek to imitate sensitive and selective mechanisms of biological systems and integrate these traits into applicable sensing platforms. Molecular imprinting technology has been extensively practiced in many domains, where it can produce various molecular recognition materials with specific recognition capabilities. Molecularly imprinted polymers (MIPs), dubbed plastic antibodies, are artificial receptors with high-affinity binding sites for a particular molecule or compound. MIPs for protein recognition are expected to have high affinity via numerous interactions between polymer matrices and multiple functional groups of the target protein. This critical review briefly describes recent advances in the synthesis, characterization, and application of MIP-based sensor platforms used to detect proteins.

Challenges and Advances of Hydrogel-Based Wearable Electrochemical Biosensors for Real-Time Monitoring of Biofluids: From Lab to Market. A Review

“…Hence, the molecular imprinting method has been employed to synthesize hydrogelbased electrodes for HWEBs. 87 Molecular imprinted polymers (MIPs) are the end-product materials obtained from the molecular imprinting procedure that includes selective recognition sites. These sites are generated by different bonding methods, such as covalent, semicovalent, or noncovalent interactions, depending on the specific approach used.…”

Molecularly Imprinted Polymer-Based Adsorbents for the Selective Removal of Pharmaceuticals from Wastewater: Adsorption Kinetics, Isotherms, and Thermodynamics Studies

“…Accordingly, MIPs offer excellent selectivity and affinity for the particular molecule utilized in the imprinting process, are very simple to synthesize, and have slower rates of degradation, which prolong the duration that their recognition sites are active at room temperature . There has been a substantial increase in interest in the idea of MIPs and its possible use in numerous areas of scientific inquiry . Due to their required selectivity, physical robustness, and thermal stability, as well as their affordability and ease of fabrication, MIPs have drawn a lot of attention and have been utilized extensively in various fields.…”

“…95 There has been a substantial increase in interest in the idea of MIPs and its possible use in numerous areas of scientific inquiry. 96 Due to their required selectivity, physical robustness, and thermal stability, as well as their affordability and ease of fabrication, MIPs have drawn a lot of attention and have been utilized extensively in various fields. MIPs were discovered to be very appropriate for use in chromatography, particularly liquid chromatography.…”

Fabrication of Metal Ion–BSA Chelation-Targeted Surface-Imprinted Particles for Protein Separation

“…However, due to the large size, complex structure, and variable conformation of proteins,protein-imprinted polymer materials still face some defects in preparation and application, such as slow mass transfer, low efficiency, few effective imprinting sites, and poor recognition. In order to solve the above problems, researchers have continuously improved the preparation process and imprinting methods of protein-imprinted materials and developed a series of new protein imprinting strategies, including protein surface imprinting technique, − molecular imprinting of smart materials, − antigen-determining base imprinting, − imprinting of magnetic materials, − and metal-chelating imprinting. , Among them, molecularly imprinted polymers (MIPs) ,− prepared by the protein surface imprinting technology are favored. The typical preparation process of such materials is as follows: the target molecule is used as a template, it is preassembled with functional monomers by the covalent or non-covalent interaction, and the template molecule is fixed in the cross-linked three-dimensional network through polymerization.…”