Molecularly imprinted membranes for an improved recognition of biomolecules in aqueous medium

Silvestri, Davide; Barbani, Niccoletta; Cristallini, Caterina; Giusti, P.; Ciardelli, Gianluca

doi:10.1016/j.memsci.2006.05.031

Cited by 76 publications

(34 citation statements)

References 41 publications

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“…Second method is to mix the nano imprinted sphere into the polymer solution and entrapped inside the matrix during coagulation. [128][129] A cryogel molecular imprinted composite membrane was synthesized by Asliyuce, anti-hepatitis B imprinted particle was first prepared, then introduced to a poly HEMA based polymer matrix by mixture and subsequent radical polymerization.…”

Section: Molecular Imprinted Composite Membrane Adsorbersmentioning

confidence: 99%

Protein-selective adsorbers by molecular imprinting via a novel two-step surface grafting method

Yin

Ulbricht

2013

J. Mater. Chem. B

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Molecularly imprinted polymers (MIP) offer in principle a robust, cost-efficient alternative to antibodies, but it is still a challenge to develop such materials for protein recognition. Here we report the molecular imprinting of a functional polymeric hydrogel layer with whole protein lysozyme as template in two-step grafting procedure by a novel initiation approach on track-etched (TE) polyethylene terephthalate (PET) and cellulose membrane surfaces.This two-step grafting strategy is based on surface functionalization with aliphatic C-Br groups which can be used as initiator for surface-initiated atom transfer radical polymerization (SI-ATRP) and photo-initiated copolymerization. At first, the scaffold poly(methacrylic acid) (PMAA) was obtained through SI-ATRP of poly(tert.-butyl methacrylate) (PtBMA) and subsequent hydrolysis. Thereafter, it was assembled with the template to form a stable PMAA/protein complex. In the second step, a polyacrylamide I declare that this dissertation represents my own work, except where due acknowledgement is made.

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Section: Molecular Imprinted Composite Membrane Adsorbersmentioning

confidence: 99%

Protein-selective adsorbers by molecular imprinting via a novel two-step surface grafting method

Yin

Ulbricht

2013

J. Mater. Chem. B

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“…In recent years, MIPs have been gained wide acceptance as new molecular recognition materials in chemical sensors, since they are more stable, less costly and easier to produce as com-pared to their biological counterparts including antibodies and enzymes [10][11][12]. Various chemical sensors have been prepared using MIPs for molecular recognition followed by appropriate signal transductions including capacitance [13], conductometry [14], amperometry [15], voltammetry [16], quartz microbalance [17] and spectroscopy [18].…”

Section: Introductionmentioning

confidence: 99%

Potentiometric sensor based on molecularly imprinted polymer for determination of melamine in milk

Liang

Zhang

Qin

2009

Sensors and Actuators B: Chemical

186

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“…Immobilized and free microspheres demonstrated the same target affi nity toward (-)-cinchonidine. Composite membrane was obtained through the deposition of theophylline imprinted poly(methyl methacrylic acid-co-acylic acid) (PMMACAA) nanoparticles in the bulk or on the surface of PMMACAA porous support membranes fabricated by the phase inversion process [76]. Th e binding capacity of the composite membrane was 6 times that of the membrane with non-imprinted nanoparticles.…”

Section: Functionalized Particles-loaded Membranes For Selective Sepamentioning

confidence: 99%

Molecular Recognition‐Driven Membrane Processes

Donato¹,

Mazzei²,

Algieri³

et al. 2014

Smart Membranes and Sensors

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High-precision, selective and effi cient systems are strongly demanded in all productive sectors, including chemical manufacturing, pharmaceutical, medical treatment, food, energy, diagnostic, analytical tools. Precision separation, conversion, formulation at molecular level may rely on various mechanisms and properties, such as molecular sieving, electrical charge, chemical solubility, molecular recognition. Membrane operation can promote mass transport, separation, conversion, formulation on the basis of the mentioned mechanisms. Biomimic and biohybrid funtionalised membranes are particularly suitable for separations based on molecular recognition.Membranes can be formed by polymers or monolythes bearing the (biomimic) recognition sites. In alternative, biomimic, biomolecules and recognition sites can be loaded on a previously formed membrane support. In such cases, surface functionalization methods can improve the loading effi ciency. Molecularly imprinted membranes; membranes bearing affi nity ligands (Antigen-Antibody, avidin/-ligand, DNA-protein, sugar-lectin, RNA-ribosome,…); membranes functionalized with zeolite and nanoparticles; membranes with immobilized enantioselective enzyme for kinetic resolutions, are among examples of selective and effi cient operations driven by molecular recognition. Th is is the mechanism by means of which molecules are able to identify each other using non-covalent -(hydrogen bonding, metal coordination, hydrophobic forces, van der Waals forces, π-π interactions and electrostatic eff ects) and covalent interactions.

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Molecularly imprinted membranes for an improved recognition of biomolecules in aqueous medium

Cited by 76 publications

References 41 publications

Protein-selective adsorbers by molecular imprinting via a novel two-step surface grafting method

Protein-selective adsorbers by molecular imprinting via a novel two-step surface grafting method

Potentiometric sensor based on molecularly imprinted polymer for determination of melamine in milk

Molecular Recognition‐Driven Membrane Processes

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