Molecularly imprinted electrochemical sensor for ochratoxin A detection in food samples

Pacheco, João G.; Castro, Mafalda; Machado, Susana; Barroso, M. Fátima; Nouws, Henri P. A.; Delerue–Matos, Cristina

doi:10.1016/j.snb.2015.03.046

Cited by 79 publications

(26 citation statements)

References 28 publications

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“…In recent years, different kinds of sensors intended for food applications have been developed, such as electrochemical sensors (Goulart, Cruz de Moraes, & Mascaro, 2016;Feng Gao et al, 2015;Liu, Xiao, Cui, & Wang, 2015;Pacheco et al, 2015;Nasirizadeh, Hajihosseini, Shekari, & Ghaani, 2015) and luminescence sensors (Fan, Shen, Wu, Wang, & Zhang, 2015;Pénicaud, Guilbert, Peyron, Gontard, & Guillard, 2010). Electrochemical sensors represent an important subclass of chemical sensors, in which an electrode is used as the transduction element.…”

Section: Sensorsmentioning

confidence: 99%

An overview of the intelligent packaging technologies in the food sector

Ghaani

Cozzolino

Castelli

et al. 2016

Trends in Food Science & Technology

414

244

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Background Intelligent packaging is the newest technology within the food packaging field. Even though this technology is still growing and not fully commercially viable, it has enormous potential to improve the safety, quality, and traceability of food products, as well as its convenience for consumers. Scope and Approach This paper first describes both the technical aspects and commercial applications of the most representative intelligent technologies-indicators, data carriers, and sensors-with special focus on systems and devices that are directly integrated into the package. Secondly, to provide useful guidelines for future research in the field, the paper discusses some important aspects that still hinder the full exploitation of intelligent technology within the food packaging industry.

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Section: Sensorsmentioning

confidence: 99%

An overview of the intelligent packaging technologies in the food sector

Ghaani

Cozzolino

Castelli

et al. 2016

Trends in Food Science & Technology

414

244

View full text Add to dashboard Cite

show abstract

“…For studying the reproducibility, three (MIP‐CPE) containing 10 % MIP were prepared and their response towards a 5x10 −5 M VCV solution was recorded determining a RSD of 6.75 %, this value indicates the possibility of fabricating new electrodes with acceptable reproducibility .…”

Section: Resultsmentioning

confidence: 99%

Voltammetric Determination of Valaciclovir Using a Molecularly Imprinted Polymer Modified Carbon Paste Electrode

et al. 2017

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A molecularly imprinted polymer (MIP) was rationally synthesized with the aid of computer based studies. The computational studies were used to screen for the most suitable template to functional monomer molar ratio. Two functional monomers were involved in the study (methacrylic acid and 4‐vinylpyridine). Four MIP ratios were synthesized in accordance with the results of the computational studies and their performance was evaluated using equilibrium rebinding assays. The MIP with the best performance was used as an additive in carbon paste electrodes for the voltammetric determination of valaciclovir (VCV). Following the optimization of voltammetric parameters, a linear response was obtained in the range of 1.0x10−6–7.0x10−4 M with a limit of detection at 4.45x10−7 M. The MIP modified carbon paste electrode was successfully applied for the determination of VCV in pure solutions and dosage form.

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“…For this reason, the development of simple, rapid, cheap and reliable analytical methods for OTA monitoring is resulting in the emergence of screening tools. A large number of rapid methods for mycotoxin analysis in food and feed have been developed, such as enzyme-linked immunosorbent assay, lateral flow devices or dipstick, flow-through immunoassay, optical and electrochemical biosensors and, more recently, aptamer-based methods [ 22 , 23 , 24 , 25 , 26 , 27 ]. Among them, fluorescent polarization immunoassay (FPIA), a promising screening method and its application, has been recently reviewed by Lippolis and Maragos [ 28 ].…”

Section: Introductionmentioning

confidence: 99%

Determination of Ochratoxin A in Rye and Rye-Based Products by Fluorescence Polarization Immunoassay

Lippolis

Porricelli

Cortese

et al. 2017

Toxins

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A rapid fluorescence polarization immunoassay (FPIA) was optimized and validated for the determination of ochratoxin A (OTA) in rye and rye crispbread. Samples were extracted with a mixture of acetonitrile/water (60:40, v/v) and purified by SPE-aminopropyl column clean-up before performing the FPIA. Overall mean recoveries were 86 and 95% for spiked rye and rye crispbread with relative standard deviations lower than 6%. Limits of detection (LOD) of the optimized FPIA was 0.6 μg/kg for rye and rye crispbread, respectively. Good correlations (r > 0.977) were observed between OTA contents in contaminated samples obtained by FPIA and high-performance liquid chromatography (HPLC) with immunoaffinity cleanup used as reference method. Furthermore, single laboratory validation and small-scale collaborative trials were carried out for the determination of OTA in rye according to Regulation 519/2014/EU laying down procedures for the validation of screening methods. The precision profile of the method, cut-off level and rate of false suspect results confirm the satisfactory analytical performances of assay as a screening method. These findings show that the optimized FPIA is suitable for high-throughput screening, and permits reliable quantitative determination of OTA in rye and rye crispbread at levels that fall below the EU regulatory limits.

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Molecularly imprinted electrochemical sensor for ochratoxin A detection in food samples

Cited by 79 publications

References 28 publications

An overview of the intelligent packaging technologies in the food sector

An overview of the intelligent packaging technologies in the food sector

Voltammetric Determination of Valaciclovir Using a Molecularly Imprinted Polymer Modified Carbon Paste Electrode

Determination of Ochratoxin A in Rye and Rye-Based Products by Fluorescence Polarization Immunoassay

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