Molecularly imprinted electrochemical sensor based on an electrode modified with an imprinted pyrrole film immobilized on a β-cyclodextrin/gold nanoparticles/graphene layer

Abstract

A novel molecularly imprinted electrochemical sensor was constructed for the sensitive detection of quercetin (Qu) based on a glassy carbon electrode (GCE) modified with β-cyclodextrin (β-CD), gold nanoparticles(AuNPs) and graphene (GR).

Surface molecular imprinted electrochemical sensor for lysozyme based on metal coordinate technology

“…2, compared with MIPs/GR/GCE, we could find bigger peak current on MIPs/IDA/GR/GCE, which was attributed to the introduction of Cu 2+ . There was metal coordination interaction between Cu 2+ and Lyz, which could immobilize more template molecules on IDA/GR/GCE than GR/GCE, leading to more imprinted caves after elution resulting more channels for the penetration of [Fe(CN) 6 ] 3-/4-through the polymer film to reach the IDA/GR/GCE for further oxidation.…”

“…. In order to investigate the performance of fabricated molecular imprinted electrochemical sensor, we measured the DPVs of different molecular imprinted electrodes (MIPs/IDA/GR/GCE,MIPs/GR/GCE) in pH 7.0 PBS containing 0.1 mM [Fe(CN) 6 ] 3-/4-. As shown in Fig.…”

“…The MIPs/IDA/GR/GCE was immersed into Lyz solution with different concentrations, and incubated for 20 min to ensure protein molecule rebound by MIPs/IDA/GR/GCE. DPV method was conducted for electrochemical determination of Lyz in PBS (pH=7.0) containing 0.1 m M [Fe(CN) 6 ] 3−/4− from 0.0 to 0.4 V at a scan rate of 100 mV/s. All electrochemical experiments were carried out at room temperature.…”

“…The classical three-electrode system consisted of an IDA modified graphene based Lyz molecular imprinted glassy carbon electrode (GCE) as the working electrode, a potassium chloride (KCl)-saturated calomel electrode (SCE) as the reference electrode, and a Pt wire electrode as the auxiliary electrode; Shanghai Kudos SK5200H ultrasonic cleaner. All electrochemical experiments were performed in PBS (pH=7.0) containing 0.1 mM [Fe(CN) 6 ] 3−/4− .…”

“…Up to now, MIECSs have been successfully used to imprint small biomolecules. 5,6 But it still remains a challenge for the imprinting of protein because of the decrease of sensitivity, due to the complexity of protein structure such as large molecular size and flexible conformation. Among the techniques used for protein imprinting, surface imprinting is currently the most popular and general method to solve the problem of diffusion limitation caused by large size of protein.…”